Zobrazeno 1 - 10
of 25
pro vyhledávání: '"Alejandro H. Orrego"'
Autor:
Alejandro H. Orrego, María Romero-Fernández, María del Carmen Millán-Linares, Justo Pedroche, José M. Guisán, Javier Rocha-Martin
Publikováno v:
Catalysts, Vol 10, Iss 6, p 676 (2020)
Very rigid supports are useful for enzyme immobilization to design continuous flow reactors and/or to work in non-conventional media. Among them, epoxy-methacrylic supports are easily functionalized with glyoxyl groups, which makes them ideal candida
Externí odkaz:
https://doaj.org/article/cabb03a2e0eb42348703b71726bf6c72
Autor:
Alejandro H. Orrego, Maria Romero-Fernández, María del Carmen Millán-Linares, María del Mar Yust, José M. Guisán, Javier Rocha-Martin
Publikováno v:
Catalysts, Vol 8, Iss 8, p 333 (2018)
Enzyme immobilization by multipoint covalent attachment on supports activated with aliphatic aldehyde groups (e.g., glyoxyl agarose) has proven to be an excellent immobilization technique for enzyme stabilization. Borohydride reduction of immobilized
Externí odkaz:
https://doaj.org/article/d4a1e42705be4b55af19bb7dea492624
Autor:
Alejandro H. Orrego, Rohollah Ghobadi, Sonia Moreno-Perez, Adriana Jaime Mendoza, Gloria Fernandez-Lorente, Jose M. Guisan, Javier Rocha-Martin
Publikováno v:
International Journal of Molecular Sciences, Vol 19, Iss 2, p 553 (2018)
Immobilized enzymes have a very large region that is not in contact with the support surface and this region could be the target of new stabilization strategies. The chemical amination of these regions plus further cross-linking with aldehyde-dextran
Externí odkaz:
https://doaj.org/article/e6a3063f5e8c4510add4c3b6490792fc
ATP‐Independent and Cell‐Free Biosynthesis of β‐Hydroxy Acids Using Vinyl Esters as Smart Substrates
Autor:
Alejandro H. Orrego, Maria Grazia Rubanu, Idania L. López, Daniel Andrés‐Sanz, Guillermo García‐Marquina, German E. Pieslinger, Luca Salassa, Fernando López‐Gallego
Publikováno v:
Angewandte Chemie International Edition. 62
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::52fd2dc7d937b0e2b67babdb0115bb4d
https://doi.org/10.1002/anie.202218312
https://doi.org/10.1002/anie.202218312
Autor:
Santiago Guisán-Ceinos, Alexandra R. Rivero, Fernando Romeo-Gella, Silvia Simón-Fuente, Silvia Gómez-Pastor, Natalia Calvo, Alejandro H. Orrego, José Manuel Guisán, Inés Corral, Francisco Sanz-Rodriguez, Maria Ribagorda
Publikováno v:
Biblos-e Archivo. Repositorio Institucional de la UAM
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We present the synthesis, photophysical properties, and biological application of nontoxic 3-azo-conjugated BODIPY dyes as masked fluorescent biosensors of hypoxia-like conditions. The synthetic methodology is based on an operationally simple N=N bon
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ce1424c580493e730d6b63b292244f11
https://pubmed.ncbi.nlm.nih.gov/35486830
https://pubmed.ncbi.nlm.nih.gov/35486830
Autor:
Mercedes Sánchez-Costa, Davide Agostino Cecchini, Aurelio Hidalgo, Jesús Fernández-Lucas, Alejandro H. Orrego
Publikováno v:
Methods in Molecular Biology ISBN: 9781071618257
Droplet microfluidics enables the ultrahigh-throughput screening of the natural or man-made genetic diversity for industrial enzymes, with reduced reagent consumption and lower costs than conventional robotic alternatives. Here we describe an example
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::5b5c13a8f46a4a20604bff782497f90f
https://doi.org/10.1007/978-1-0716-1826-4_2
https://doi.org/10.1007/978-1-0716-1826-4_2
Autor:
Davide Agostino, Cecchini, Mercedes, Sánchez-Costa, Alejandro H, Orrego, Jesús, Fernández-Lucas, Aurelio, Hidalgo
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 2397
Droplet microfluidics enables the ultrahigh-throughput screening of the natural or man-made genetic diversity for industrial enzymes, with reduced reagent consumption and lower costs than conventional robotic alternatives. Here we describe an example
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::8be8d15e130a5f7f3636b89a6f5c37f2
https://pubmed.ncbi.nlm.nih.gov/34813057
https://pubmed.ncbi.nlm.nih.gov/34813057
Autor:
José Berenguer, Alejandro H. Orrego, Daniel Andrés-Sanz, Fernando López-Gallego, Jose M. Guisan, Mercedes Sánchez-Costa, Javier Rocha-Martín, Susana Velasco-Lozano
Publikováno v:
Biblos-e Archivo. Repositorio Institucional de la UAM
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Catalysis Science & Technology
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Catalysis Science & Technology
β-Hydroxyesters are essential building blocks utilised by the pharmaceutical and food industries in the synthesis of functional products. Beyond the conventional production methods based on chemical catalysis or whole-cell synthesis, the asymmetric
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3e17285a3db597403f77b87df956ebd1
https://doi.org/10.5281/zenodo.4697225
https://doi.org/10.5281/zenodo.4697225
Autor:
José M. Fraile, Javier Rocha-Martín, Jose M. Guisan, Fernando López-Gallego, Alejandro H. Orrego, Sonia Moreno-Pérez, Sandro Martins de Oliveira, Susana Velasco-Lozano
Publikováno v:
Digital.CSIC. Repositorio Institucional del CSIC
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The functionalization of the internal surface of macroporous carriers with glyoxyl groups has proven to highly stabilize a large variety of enzymes through multipoint covalent immobilization. In this work, we have translated the surface chemistry dev
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b47b3341797b710c46b653add15085fe
https://pubmed.ncbi.nlm.nih.gov/33423456
https://pubmed.ncbi.nlm.nih.gov/33423456
Publikováno v:
International journal of biological macromolecules. 157
Glycosylation is one of the most efficient biocompatible methodologies to enhance the water solubility of natural products, and therefore their bioavailability. The excellent regio- and stereoselectivity of nucleotide sugar-dependent glycosyltransfer
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2f0b92fa08d40273ddb94673731cee30
https://pubmed.ncbi.nlm.nih.gov/32344088
https://pubmed.ncbi.nlm.nih.gov/32344088