Zobrazeno 1 - 10
of 23
pro vyhledávání: '"Alec A Desai"'
Autor:
Ghasidit Pornnoppadol, Boya Zhang, Alec A Desai, Anthony Berardi, Henriette A Remmer, Peter M Tessier, Colin F Greineder
Publikováno v:
PLoS ONE, Vol 16, Iss 10, p e0252558 (2021)
The identification of antibody variable regions in the heavy (VH) and light (VL) chains from hybridomas is necessary for the production of recombinant, sequence-defined monoclonal antibodies (mAbs) and antibody derivatives. This process has received
Externí odkaz:
https://doaj.org/article/2422a6f031f8494c9006d47c01d9a2c4
Autor:
Emily K. Makowski, Patrick C. Kinnunen, Jie Huang, Lina Wu, Matthew D. Smith, Tiexin Wang, Alec A. Desai, Craig N. Streu, Yulei Zhang, Jennifer M. Zupancic, John S. Schardt, Jennifer J. Linderman, Peter M. Tessier
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-14 (2022)
Optimising antibody properties such as affinity can be detrimental to other key properties. Here the authors use machine learning to simplify the identification of antibodies with co-optimal levels of affinity and specificity for a clinical-stage ant
Externí odkaz:
https://doaj.org/article/0014043dced04499aebddc31d0764ef0
Autor:
John S. Schardt, Ghasidit Pornnoppadol, Alec A. Desai, Kyung Soo Park, Jennifer M. Zupancic, Emily K. Makowski, Matthew D. Smith, Hongwei Chen, Mayara Garcia de Mattos Barbosa, Marilia Cascalho, Thomas M. Lanigan, James J. Moon, Peter M. Tessier
Publikováno v:
Scientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
Abstract Monoclonal antibodies that target SARS-CoV-2 with high affinity are valuable for a wide range of biomedical applications involving novel coronavirus disease (COVID-19) diagnosis, treatment, and prophylactic intervention. Strategies for the r
Externí odkaz:
https://doaj.org/article/75354dc4a1d54b489cf8d0e0fd26f7ef
Autor:
Emily K. Makowski, Hongwei Chen, Matthew Lambert, Eric M. Bennett, Nicole S. Eschmann, Yulei Zhang, Jennifer M. Zupancic, Alec A. Desai, Matthew D. Smith, Wenjia Lou, Amendra Fernando, Timothy Tully, Christopher J. Gallo, Laura Lin, Peter M. Tessier
Publikováno v:
mAbs, Vol 14, Iss 1 (2022)
Self-association governs the viscosity and solubility of therapeutic antibodies in high-concentration formulations used for subcutaneous delivery, yet it is difficult to reliably identify candidates with low self-association during antibody discovery
Externí odkaz:
https://doaj.org/article/b7a6b619288444029f50f9d427dbd60c
Facile isolation of high-affinity nanobodies from synthetic libraries using CDR-swapping mutagenesis
Publikováno v:
STAR Protocols, Vol 3, Iss 1, Pp 101101- (2022)
Summary: The generation of high-affinity nanobodies for diverse biomedical applications typically requires immunization or affinity maturation. Here, we report a simple protocol using complementarity-determining region (CDR)-swapping mutagenesis to i
Externí odkaz:
https://doaj.org/article/35b4cd6716784b719aaf0326f1a20c56
Autor:
Weishu Wu, Xiaotian Tan, Jennifer Zupancic, John S. Schardt, Alec A. Desai, Matthew D. Smith, Jie Zhang, Liangzhi Xie, Maung Khaing Oo, Peter M. Tessier, Xudong Fan
Publikováno v:
Anal Chem
Neutralizing monoclonal antibodies and nanobodies have shown promising results as potential therapeutic agents for COVID-19. Identifying such antibodies and nanobodies requires evaluating the neutralization activity of a large number of lead molecule
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e3293a0dfd611e7b97bfbe71ece8745b
https://doi.org/10.1021/acs.analchem.2c00062
https://doi.org/10.1021/acs.analchem.2c00062
Autor:
Seth D. Ludwig, Lilia A. Rabia, Yulei Zhang, Priyanka Gupta, Peter M. Tessier, Matthew D. Smith, Alec A. Desai, Lina Wu
Publikováno v:
Mol Pharm
The ability of antibodies to recognize their target antigens with high specificity is fundamental to their natural function. Nevertheless, therapeutic antibodies display variable and difficult-to-predict levels of non-specific and self-interactions t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9f556ac4be531494e7d7a1afe925947e
https://doi.org/10.1021/acs.molpharmaceut.0c00257
https://doi.org/10.1021/acs.molpharmaceut.0c00257
Publikováno v:
Methods in Molecular Biology ISBN: 9781071622841
Methods Mol Biol
Methods Mol Biol
Conformational antibodies specific for amyloid-forming peptides and proteins are important for a range of biomedical applications, including detecting, inhibiting, and potentially treating protein aggregation disorders ranging from Alzheimer's to Par
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::63dee8dc620e4a18f26c56bb7f2d799a
https://doi.org/10.1007/978-1-0716-2285-8_22
https://doi.org/10.1007/978-1-0716-2285-8_22
Publikováno v:
mAbs
article-version (VoR) Version of Record
article-version (VoR) Version of Record
The rapidly evolving nature of antibody drug development has resulted in technologies that generate vast numbers (hundreds to thousands) of lead antibody candidates during early discovery. These candidates must be rapidly pared down to identify the m
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::080399ceb0886101fa6127840ccd17df
https://pubmed.ncbi.nlm.nih.gov/34313552
https://pubmed.ncbi.nlm.nih.gov/34313552
Autor:
Boya Zhang, Anthony Berardi, Colin F. Greineder, Peter M. Tessier, Alec A. Desai, Henriette A. Remmer, Ghasidit Pornnoppadol
Publikováno v:
PLoS ONE
PLoS ONE, Vol 16, Iss 10, p e0252558 (2021)
PLoS ONE, Vol 16, Iss 10, p e0252558 (2021)
The identification of antibody variable regions in the heavy (VH) and light (VL) chains from hybridomas is necessary for the production of recombinant, sequence-defined monoclonal antibodies (mAbs) and antibody derivatives. This process has received
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aa42989848b04e351455ff2a9bd334f3
https://doi.org/10.1101/2021.05.19.444780
https://doi.org/10.1101/2021.05.19.444780