Zobrazeno 1 - 10
of 43
pro vyhledávání: '"Alberto J. Napuli"'
Publikováno v:
Acta Crystallographica Section F
One third of the world’s human population is infected with M. tuberculosis, the etiological agent responsible for tuberculosis (TB). Here, the solution structure of the small iron-binding protein from this organism, rubredoxin B (Rv3250c), is repor
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::215795b8655ce5ea1f84ef173c86aaff
https://doi.org/10.1107/s1744309111008189
https://doi.org/10.1107/s1744309111008189
Autor:
Christian Olsen, Alberto J. Napuli, Wesley C. Van Voorhis, Isabelle Phan, Peter J. Myler, Sandhya Subramanian, Jan Abendroth, Bart L. Staker, Robin Stacy
Publikováno v:
Acta Crystallographica Section F: Structural Biology and Crystallization Communications
The R. prowazekii 3-ketoacyl-(acyl-carrier-protein) reductase is similar to those from other prokaryotic pathogens but differs significantly from the mammalian orthologue, strengthening its case as a potential drug target.
Rickettsia prowazekii,
Rickettsia prowazekii,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::14896ec67892c9f414977b74cf801067
https://doi.org/10.1107/s1744309111030673
https://doi.org/10.1107/s1744309111030673
Publikováno v:
Acta Crystallographica Section F
B. melitensis is a NIAID Category B microorganism that is responsible for brucellosis and is a potential agent for biological warfare. Here, the solution structure of the 116-residue arsenate reductase-related protein Bm-YffB (BR0369) from this organ
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7b62cb118b31fab40a80ec0f25f9e97f
https://doi.org/10.1107/s1744309111006336
https://doi.org/10.1107/s1744309111006336
Autor:
Thomas E. Edwards, Alberto J. Napuli, Bart L. Staker, Janhavi Bhandari, Banumathi Sankaran, Wesley C. Van Voorhis, Anna Gardberg, Jan Abendroth, Peter J. Myler, Lance Stewart, Shellie H. Dieterich
Publikováno v:
Acta Crystallographica Section F: Structural Biology and Crystallization Communications
The structure of a β-lactamase-like protein from B. melitensis was solved independently using two data sets with anomalous signal. Anomalous Fourier maps could confirm the identity of two metal ions in the active site. AMP-bound and GMP-bound struct
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f6f0286e816f209d93575c236e0401ba
https://doi.org/10.1107/s1744309111010220
https://doi.org/10.1107/s1744309111010220
Autor:
Christian Olsen, Wenjin Guo, Thomas E. Edwards, Isabelle Phan, Ariel Abramov, Peter J. Myler, Micah Ferrell, W. C. Van Voorhis, Lance Stewart, Kaitlin Thompkins, Darren W. Begley, Banumathi Sankaran, Yang Zhang, Robin Stacy, Alberto J. Napuli
Publikováno v:
Acta Crystallographica Section F: Structural Biology and Crystallization Communications
The 1.6 Å resolution crystal structure of nitrilotriacetate monooxygenase component B (NTA-MoB) from M. thermoresistibile is presented, revealing a highly conserved C-terminal tail that may modulate the activity of NTA-MoB in mycobacteria.
Myco
Myco
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::542cc0789b61333cb1cf38759d4ce0a8
https://doi.org/10.1107/s1744309111012541
https://doi.org/10.1107/s1744309111012541
Autor:
Wesley C. Van Voorhis, Ryan Choi, David J. Leibly, Angela Kelley, Stephen N. Hewitt, Alberto J. Napuli
Publikováno v:
Acta Crystallographica Section F: Structural Biology and Crystallization Communications
An overview of the methods used for high-throughput cloning and protein-expression screening of SSGCID hexahistidine recombinant proteins is provided. It is demonstrated that screening for recombinant proteins that are highly recoverable from immobil
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::431acbb1d32a68db528319a9e3583b10
https://doi.org/10.1107/s1744309111017374
https://doi.org/10.1107/s1744309111017374
Autor:
Alberto J. Napuli, Thomas E. Edwards, Angela Kelley, Janhavi Bhandari, Wesley C. Van Voorhis, Cassie M. Bryan, Ryan Choi, David J. Leibly, Lance Stewart
Publikováno v:
Acta Crystallographica Section F: Structural Biology and Crystallization Communications
An overview of the standard SSGCID protein-purification protocol is given and success rates and cleavage alternatives are discussed.
The establishment of an efficient and reliable protein-purification pipeline is essential for the success of str
The establishment of an efficient and reliable protein-purification pipeline is essential for the success of str
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fb7f82af80f6d1a0a7c8e27fb7542897
http://europepmc.org/articles/PMC3169394
http://europepmc.org/articles/PMC3169394
Autor:
Wim G. J. Hol, Erkang Fan, Jennifer M. Ross, Natasha Mueller, Wesley C. Van Voorhis, Christophe L. M. J. Verlinde, Zhongsheng Zhang, Frank H. Zucker, Sayaka Shibata, Jaclyn Delarosa, Alberto J. Napuli, Ethan A. Merritt, Konstantin V. Korotkov, Angela Kelley, Frederick S. Buckner
Publikováno v:
Analytical and Bioanalytical Chemistry. 401:1585-1591
Ultrafiltration provides a generic method to discover ligands for protein drug targets with millimolar to micromolar K(d), the typical range of fragment-based drug discovery. This method was tailored to a 96-well format, and cocktails of fragment-siz
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1012bc40b628184e18c087fb4045713f
https://doi.org/10.1007/s00216-011-5225-7
https://doi.org/10.1007/s00216-011-5225-7
Autor:
Frank H. Zucker, Alberto J. Napuli, Erkang Fan, Frederick S. Buckner, Lisa J. Castaneda, Wim G. J. Hol, Wesley C. Van Voorhis, Christophe L. M. J. Verlinde, Jessica E. Kim, Ethan A. Merritt, Eric T. Larson, Zhongsheng Zhang
Publikováno v:
Journal of Molecular Biology. 409:159-176
The single tyrosyl-tRNA synthetase (TyrRS) gene in trypanosomatid genomes codes for a protein that is twice the length of TyrRS from virtually all other organisms. Each half of the double-length TyrRS contains a catalytic domain and an anticodon-bind
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::53a6ab798c99f767dec2a29a9fa66fc2
https://doi.org/10.1016/j.jmb.2011.03.026
https://doi.org/10.1016/j.jmb.2011.03.026
Autor:
Kuzma V. Kovzun, Wim G. J. Hol, Frederick S. Buckner, Ranae Pefley, Christophe L. M. J. Verlinde, Wesley C. Van Voorhis, Angela Kelley, J. Robert Gillespie, Zhongsheng Zhang, Frank H. Zucker, Sayaka Shibata, Alberto J. Napuli, Jocelyn Lam, Erkang Fan, Ethan A. Merritt
Publikováno v:
Antimicrobial Agents and Chemotherapy. 55:1982-1989
Human African trypanosomiasis continues to be an important public health threat in extensive regions of sub-Saharan Africa. Treatment options for infected patients are unsatisfactory due to toxicity, difficult administration regimes, and poor efficac
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b85edc6a533574a55d13f37b64e40a6f
https://doi.org/10.1128/aac.01796-10
https://doi.org/10.1128/aac.01796-10