Zobrazeno 1 - 7
of 7
pro vyhledávání: '"Albert Ville"'
Autor:
Marie-Françoise Hacques, Albert Ville, Roger Couturier, Guy Favre-Bonvin, Pascal Michalon, Christian Marion
Publikováno v:
Biochemical and biophysical research communications. 167(1)
A new method consisting of a two-step activation was developed in order to covalently immobilize enzymes on calibrate nylon 66 spheres. This efficient method associates for the first time peptide bond cleavage and O-alkylation of the support. Optimal
Publikováno v:
Analytical Letters. 18:2209-2218
A rapid and sensitive method for the quantitation of microgram antibodies immobilized on polystyrene is proposed. A procedure of protein staining on polystyrene with coomassie, brilliant blue G 250 follows Beer's law after release of the coloured com
Publikováno v:
Die Makromolekulare Chemie. 187:1603-1610
Antibodies were immobilized by covalent linkage on polystyrene balls for use in solid phase enzyme-linked immunoassays: application for detection of K 99+E. Coli in calves. The method eliminates the problem of antibody desorption noted in coventional
Publikováno v:
Biochimie. 56:523-527
Summary In order to study the interaction of small cations with rat liver ribosomal RNA's, different desalting methods have been examined. Gel filtration has a too low desalting efficiency. Ion exchanges resins, dialysis and electrodialysis are effic
Publikováno v:
Biochimie. 56:1197-1201
Summary The binding of Mg ++ , Li + , Na + , K + and Cs + by rat liver rRNA is studied through a conductometric technique. The stoechiometry of the Mg ++ binding obtained by this method (one Mg ++ equivalent bound per P atom), is identical with the n
Publikováno v:
Die Makromolekulare Chemie. 188:1305-1312
A new method of immobilization of low-molecular-weight compounds or antibodies on nylon 66 was developed. Secondary amide groups of nylon 66 were converted into secondary imidate groups by O-alkylation with sulfuric acid and methanol. The methoxycarb
Publikováno v:
Biochimie. 53:439-446
Summary The binding of Cu2+ on bovine pancreatic ribonuclease, ribonuclease A and ribonuclease B has been studied in salt-free solutions (pH = 6,6) by a conductometric method. Both ribonuclease A and B have a strong binding site ; the association con