Zobrazeno 1 - 10
of 87
pro vyhledávání: '"Albert M. Bobst"'
Autor:
Manju Varma, H.D.A. Lindquist, Michael W. Ware, Frank W. Schaefer, Jeffery Dean Hester, Albert M. Bobst
Publikováno v:
Molecular and Cellular Probes. 16:435-444
This study describes fluorogenic 5′ nuclease PCR assays suitable for rapid, sensitive, high-throughput detection of the human-pathogenic microsporidial species Encephalitozoon hellem, E. cuniculi and E.intestinalis . The assays utilize species-spec
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f94576a85872fa80134cb6bf84fc068e
https://doi.org/10.1006/mcpr.2002.0442
https://doi.org/10.1006/mcpr.2002.0442
Publikováno v:
Free Radical Research. 36:491-498
Nitroxide-labeled nucleic acids are used as a molecular size sensor to identify as few as one genome under polymerase chain reaction (PCR) conditions by electron paramagnetic resonance (EPR) spectroscopy. DNA identification is based on differences in
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::07f96a7f0c86545a03a616f58f773616
https://doi.org/10.1080/10715760290025870
https://doi.org/10.1080/10715760290025870
Publikováno v:
The Journal of Physical Chemistry B. 104:5372-5381
A systematic analysis is presented of the ESR spectra from DNA oligomers of a range of sizes, including a polymer, spin-labeled with nitroxide moieties attached by different tethers. The complexity of the DNA dynamics is dealt with by the use of the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::67e85bba5df98db4d4b9ff104b57f6f7
https://doi.org/10.1021/jp994219f
https://doi.org/10.1021/jp994219f
Publikováno v:
Journal of Catalysis. 192:174-184
The overall purpose of this study was to investigate the effects of the magnesium and lithium precursors and the catalyst surface properties on the catalytic performance in the oxidative methylation of acetonitrile. The performance of the catalysts f
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::3cd30d28290cc392bc0fcd7ab0de4ce8
https://doi.org/10.1006/jcat.2000.2852
https://doi.org/10.1006/jcat.2000.2852
Publikováno v:
Journal of Molecular and Cellular Cardiology. 31:113-121
There is increasing evidence that reactive oxygen species (ROS) contribute to post-ischemic reperfusion injury, but determination of the specific ROS involved has proven elusive. In the present study electron paramagnetic resonance (EPR) spectroscopy
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::110add2249df5a826180551541927ea3
https://doi.org/10.1006/jmcc.1998.0850
https://doi.org/10.1006/jmcc.1998.0850
Publikováno v:
Biochemistry. 35:9309-9313
Spectroscopic evidence of a DNA bend in solution is presented by analyzing model 15-mer duplexes spin-labeled with the five-atom-tethered nitroxide DUAP located in the major groove. Three 15-mers containing AATT with DUAP enzymatically incorporated i
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::46cd148dff76ffeafb5973a094b4b9a5
https://doi.org/10.1021/bi9609230
https://doi.org/10.1021/bi9609230
Rigidity of a B–Z Region Incorporated into a Plasmid as Monitored by Electron Paramagnetic Resonance
Publikováno v:
Archives of Biochemistry and Biophysics. 324:357-366
Electron paramagnetic resonance spectroscopy was employed to monitor the dynamics associated with a B-Z transition in both a linear (dG-dC)n and a modified pUC8 plasmid. A spin label consisting of cytidine substituted in position C5 with an 11-atom-t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::de5bd9823ce6558689d411b8dd076ad6
https://doi.org/10.1006/abbi.1995.0048
https://doi.org/10.1006/abbi.1995.0048
Autor:
Albert M. Bobst, Robert S. Keyes
Publikováno v:
Biophysical Chemistry. 45:281-303
An EPR competition formalism is developed which provides relative affinities of proteins for nucleic acids. Two models for analyzing protein-nucleic acid interactions, one assuming independent binding sites (Model 1) and the other considering site ov
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::be7068d96029a9b63a45d146f9b43233
https://doi.org/10.1016/0301-4622(93)80009-8
https://doi.org/10.1016/0301-4622(93)80009-8
Autor:
Albert M. Bobst, Jauh-Lin Duh
Publikováno v:
Helvetica Chimica Acta. 74:739-747
Oligothymidylates (oligo(dT)'s) with the sequence-specifically-incorporated one-atom-tethered C(5)-nitio-xide-labeled nucleoside 1 were synthesized by the phosphotriester method. Some modifications of the protocol were required to account for the che
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::e518a7e85c396bd3d1891549e71f2836
https://doi.org/10.1002/hlca.19910740407
https://doi.org/10.1002/hlca.19910740407
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology. 1078:199-207
A direct quantitative determination by EPR of the nucleic acid binding affinity relationship of the single-stranded DNA-binding protein (SSB) from Escherichia coli at close to physiological NaCl concentration is reported. Titrations of (DUAP, dT)n, a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b580a74406cdf1017ca43c67b563e86d
https://doi.org/10.1016/0167-4838(91)99010-p
https://doi.org/10.1016/0167-4838(91)99010-p