Zobrazeno 1 - 10
of 50
pro vyhledávání: '"Alban Massip"'
Publikováno v:
Biology of Reproduction. 69:1707-1713
Early embryonic cleavages are mostly regulated by maternal components then control of development progressively depends on newly synthesized zygotic products. The timing of the first cleavages is a way to assess embryo quality. The goal of this study
Autor:
Alban Massip
Publikováno v:
Reproduction Nutrition Development. 43:325-330
The cryopreservation of oocytes of most animal species remains a challenge due to their complex structure. Equilibrium freezing is not satisfactory because oocytes seem to be damaged by exposure for several minutes to temperatures near 0 degrees C. T
Publikováno v:
Biology of Reproduction. 65:1127-1134
This study compares the effects of conventional controlled-rate freezing and vitrification on the morphology and metabolism of in vitro-produced bovine blastocysts. Day 7 expanded blastocysts cultured in synthetic oviduct fluid with 5% fetal calf ser
Publikováno v:
European Journal of Morphology. 39:73-79
The effect of serum added to a modified SOF medium on pulsatile activity and hatching of in vitro produced cow blastocysts was investigated by time-lapse cinematography. Embryos were generated from abattoir material and cultured in mSOF without serum
Autor:
Isabelle Donnay, Jean-Magloire Feugang, Anne Van Langendonckt, Alban Massip, Anne-Sophie Lequarré, Olivier Malhomme, F. Dessy
Publikováno v:
Molecular Reproduction and Development. 58:45-53
Temporal pattern of expression of Cu/Zn and Mn superoxide dismutases (SODs) was investigated in bovine oocytes and embryos produced in vitro in two different culture conditions and in vivo after superovulation. SODs were examined at a transcriptional
Publikováno v:
Cryobiology. 41:106-115
The osmotic behavior of bovine blastocysts produced in vitro was filmed during exposure to and dilution of cryoprotectant solutions used for vitrification. The relationship between the changes in the diameter of embryos and their subsequent survival
Autor:
Valérie Majerus, S. Kaidi, Alban Massip, E M Ferguson, Franz Dessy, Isabelle Donnay, Anne-Sophie Lequarré
Publikováno v:
Molecular Reproduction and Development. 57:346-352
Embryos derived from calf oocytes were compared with adult cow oocyte-derived embryos (1) by studying the kinetics of embryo development using time-lapse cinematography (2) by evaluating the ratio between inner cell mass (ICM) and trophectoderm (TE)
Publikováno v:
Theriogenology. 52:515-525
Embryo quality of in vitro-produced bovine blastocysts was assessed at several steps of a vitrification procedure in which glycerol and ethylene glycol were used as cryoprotectants (3-step equilibration with cryoprotectants followed by vitrification,
Publikováno v:
Molecular Reproduction and Development. 52:149-157
The impact of oxidative stress on the in vitro development of bovine embryos in synthetic oviduct fluid medium (mSOF) was assessed by using H2O2 as a stress inducer. In a preliminary experiment, a chemiluminescent method was used to measure the antio
Publikováno v:
Theriogenology. 48:1387-1395
This study aimed at producing viable bovine blastocysts by IVM, IVF and IVC, in media without addition of bovine-originated products. Bovine serum albumin (BSA) was replaced by polyvinyl alcohol in washing media, fetal calf serum (FCS) by rabbit seru