Zobrazeno 1 - 10
of 21
pro vyhledávání: '"Alan R. Koch"'
Publikováno v:
Journal of Experimental Biology. 202:247-252
The ‘stomach’ region of the larval mosquito midgut is divided into histologically distinct anterior and posterior regions. Anterior stomach perfused symmetrically with saline in vitro had an initial transepithelial potential (TEP) of −66 mV (lu
Publikováno v:
Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology. 121:181-187
Manduca sexta anterior midgut alkalinizes its luminal contents in vivo, achieving pH values up to 12 with rates of up to 81.4 μ Eq OH − h −1 . The alkalinization rate dropped to 3.0 μ Eq h −1 within minutes of mounting the tissue in an Ussing
Autor:
Steven R. Reiken, William C. Davis, David F. Moffett, Bernard J. Van Wie, Alan R. Koch, Moris Silber, Himawan Sutisna
Publikováno v:
Biosensors and Bioelectronics. 11:91-102
Recent results show that bispecific antibodies can be used to tailor the selectivity of nicotinic acetylcholine receptors for biosensing purposes. The nicotinic acetylcholine receptors reconstituted in bilayer lipid membranes are inactivated when two
Publikováno v:
Journal of Experimental Biology. 198:2103-2113
The midgut epithelium of lepidopteran insect larvae contains characteristic goblet cells possessing an apical cavity surrounded by goblet cell apical membrane (GCAM) and guarded from the gut lumen by a valve-like structure. The currently accepted mod
Autor:
Numan S. Dogan, Liangjun Liu, Moris Silber, Mete Eray, Bernard J. Van Wie, Alan R. Koch, David F. Moffett
Publikováno v:
Biosensors and Bioelectronics. 9:343-351
Microfabrication is used to realize a polyimide aperture for highly stable bilayer lipid membranes (BLMs). The microfabrication process used in this work is compatible with standard processes used in microelectronics. The physical features of the ape
Autor:
Alan R. Koch, David F. Moffett
Publikováno v:
Journal of Experimental Biology. 172:403-415
In the midgut of larval lepidopteran insects, goblet cells are believed to secrete K+; the proposed mechanism involves an electrogenic K+/nH+ (n>l) antiporter coupled to primary active transport of H+ by a vacuolar-type ATPase. Goblet cells have a pr
Autor:
Alan R. Koch, David F. Moffett
Publikováno v:
Journal of Experimental Biology. 157:243-256
The isolated posterior midgut of the tobacco hornworm maintains a vigorous transepithelial K+ transport from the hemolymphal side to the lumen side at a rate accurately measured by its short-circuit current. Previous studies using the K+ channel bloc
Publikováno v:
Journal of Experimental Biology. 155:403-414
In the larval lepidopteran midgut, the major energy-requiring step of transepi-thelial K+ secretion occurs across the goblet cell apical membrane. Studies of vesicles of goblet cell apical membrane suggest that K+ secretion across this membrane is a
Publikováno v:
Proceedings of the 15th Annual International Conference of the IEEE Engineering in Medicine and Biology Societ.
Publikováno v:
Biotechnology progress. 14(2)
In this study, we prepared bifunctional biopolymers for development of a novel liposomal immunosensing element. These biopolymers were produced such that a rat monoclonal antibody fragment Fab' was linked to a cardiac protein Troponin I (TnI) peptide