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pro vyhledávání: '"Alan P. Wolffe"'
Autor:
Alan P. Wolffe
The Third Edition of Chromatin: Structure and Function brings the reader up-to-date with the remarkable progress in chromatin research over the past three years. It has been extensively rewritten to cover new material on chromatin remodeling, histone
Autor:
Andrew C. Jamieson, Lei Zhang, Casey C. Case, Reed Hickey, David F. Meoli, Anjali K. Nath, Frank J. Giordano, Bingliang Chen, Edward J. Rebar, Yan Huang, Lei Xu, S. Kaye Spratt, Alan P. Wolffe, Yuxin Liang, Sameer K. Nath
Publikováno v:
Nature Medicine. 8:1427-1432
The relationship between the structure of zinc-finger protein (ZFP) transcription factors and DNA sequence binding specificity has been extensively studied. Advances in this field have made it possible to design ZFPs de novo that will bind to specifi
Publikováno v:
Genes & Development. 16:1540-1554
Stably integrated transgenes flanked by the chicken β-globin HS4 insulator are protected against chromosomal position effects and gradual extinction of expression during long-term propagation in culture. To investigate the mechanism of action of thi
Autor:
Edward J. Rebar, Pei-Xiang Li, Yuxin Liang, Xiao-Yong Li, Casey C. Case, Yuanyue Zhou, Alan P. Wolffe, Bingliang Chen
Publikováno v:
Journal of Biological Chemistry. 277:20087-20094
Unregulated expression of vascular endothelial growth factor-A (VEGF-A) plays an important role in tumor growth. We have identified a cell type-specific enhancer, HS-1100, that contributes to VEGF-A transcriptional activation in tumorigenic glioblast
Publikováno v:
Genes & Development. 16:27-32
To determine functional differences between the two splice variants of PPARgamma (gamma1 and gamma2), we sought to selectively repress gamma2 expression by targeting engineered zinc finger repressor proteins (ZFPs) to the gamma2-specific promoter, P2
Autor:
Stephen P. Eisenberg, S. Kaye Spratt, Alan P. Wolffe, Andrew C. Jamieson, Matthew C. Mendel, Pei-Xiang Li, Qiang Liu, Yuxin Liang, Lei Zhang, Bingliang Chen, Xiaohong Zhong, Pei-Qi Liu, Hong Qi, Casey C. Case, Edward J. Rebar, Ya-Li Lee
Publikováno v:
Journal of Biological Chemistry. 276:11323-11334
We have mapped conserved regions of enhanced DNase I accessibility within the endogenous chromosomal locus of vascular endothelial growth factor A (VEGF-A). Synthetic zinc finger protein (ZFP) transcription factors were designed to target DNA sequenc
Publikováno v:
Plant Molecular Biology. 46:121-129
Chromatin structure, the organized packaging of DNA with histones in the nucleus, is now seen as a dynamic fabric that changes with development. Here, we use studies on the phaseolin (phas) gene that encodes a seed protein to show how chromatin struc
Autor:
Fyodor D. Urnov, Alan P. Wolffe
Publikováno v:
Journal of Mammary Gland Biology and Neoplasia. 6:153-167
Epigenetic regulation involves the maintenance of a particular state of gene expression--most commonly, repression--in the face of repeated mitosis, and frequently meiosis. Remarkably, changes in such heritable expression states occur without an alte
Publikováno v:
Molecular Biology Reports. 28:209-215
Transcriptional activity is closely associated with DNA methylation and chromatin remodelling. Evidence is emerging that a family of methylation specific (methyl-CpG binding domain, MBD) proteins have the capacity to bind to methylated sequences and
Autor:
Alan P. Wolffe, Esteban Ballestar
Publikováno v:
European Journal of Biochemistry. 268:1-6
CpG methylation, the most common epigenetic modification of vertebrate genomes, is primarily associated with transcriptional repression. MeCP2, MBD1, MBD2, MBD3 and MBD4 constitute a family of vertebrate proteins that share the methyl-CpG-binding dom