Zobrazeno 1 - 10
of 295
pro vyhledávání: '"Alan G Hinnebusch"'
Publikováno v:
eLife, Vol 13 (2024)
We have developed a deep sequencing-based approach, Rec-Seq, that allows simultaneous monitoring of ribosomal 48S preinitiation complex (PIC) formation on every mRNA in the translatome in an in vitro reconstituted system. Rec-Seq isolates key early s
Externí odkaz:
https://doaj.org/article/379dbd1d0a6640ed96c6e0b8d84b47d5
Publikováno v:
eLife, Vol 12 (2024)
Initiating translation of most eukaryotic mRNAs depends on recruitment of methionyl initiator tRNA (Met-tRNAi) in a ternary complex (TC) with GTP-bound eukaryotic initiation factor 2 (eIF2) to the small (40S) ribosomal subunit, forming a 43S preiniti
Externí odkaz:
https://doaj.org/article/bf7e64a34aed47fe943320241c4589a0
Autor:
Anil Kumar Vijjamarri, Xiao Niu, Matthew D Vandermeulen, Chisom Onu, Fan Zhang, Hongfang Qiu, Neha Gupta, Swati Gaikwad, Miriam L Greenberg, Paul J Cullen, Zhenguo Lin, Alan G Hinnebusch
Publikováno v:
eLife, Vol 12 (2023)
Degradation of most yeast mRNAs involves decapping by Dcp1/Dcp2. DEAD-box protein Dhh1 has been implicated as an activator of decapping, in coupling codon non-optimality to enhanced degradation, and as a translational repressor, but its functions in
Externí odkaz:
https://doaj.org/article/aa2f0c85f238450c866b4e82f7ac3f97
Publikováno v:
PLoS Genetics, Vol 18, Iss 7, p e1010277 (2022)
The nucleosome remodeling complexes (CRs) SWI/SNF, RSC, and Ino80C cooperate in evicting or repositioning nucleosomes to produce nucleosome depleted regions (NDRs) at the promoters of many yeast genes induced by amino acid starvation. We analyzed mut
Externí odkaz:
https://doaj.org/article/35e9e1f5f5ce4e818df401b7de78ad09
Autor:
Swati Gaikwad, Fardin Ghobakhlou, David J Young, Jyothsna Visweswaraiah, Hongen Zhang, Alan G Hinnebusch
Publikováno v:
eLife, Vol 10 (2021)
In eukaryotes, 43S preinitiation complex (PIC) formation is a rate-determining step of translation. Ribosome recycling following translation termination produces free 40S subunits for re-assembly of 43S PICs. Yeast mutants lacking orthologs of mammal
Externí odkaz:
https://doaj.org/article/e39deb7ff5ef4afcb85e31f01fb99d02
Publikováno v:
eLife, Vol 9 (2020)
Yeast DEAD-box helicase Ded1 stimulates translation initiation, particularly of mRNAs with structured 5'UTRs. Interactions of the Ded1 N-terminal domain (NTD) with eIF4A, and Ded1-CTD with eIF4G, subunits of eIF4F, enhance Ded1 unwinding activity and
Externí odkaz:
https://doaj.org/article/75675e0e4795445bbe9ffffe87494d14
Publikováno v:
PLoS Genetics, Vol 15, Iss 7, p e1008299 (2019)
[This corrects the article DOI: 10.1371/journal.pgen.1007806.].
Externí odkaz:
https://doaj.org/article/7d3c19eef28c4c19a126369a04afc2cb
Publikováno v:
PLoS Genetics, Vol 14, Iss 12, p e1007806 (2018)
Scd6 protein family members are evolutionarily conserved components of translationally silent mRNA granules. Yeast Scd6 interacts with Dcp2 and Dhh1, respectively a subunit and a regulator of the mRNA decapping enzyme, and also associates with transl
Externí odkaz:
https://doaj.org/article/d9204123e7ba496e9c070f366034b0e6
Autor:
Jose Luis Llácer, Tanweer Hussain, Adesh K Saini, Jagpreet Singh Nanda, Sukhvir Kaur, Yuliya Gordiyenko, Rakesh Kumar, Alan G Hinnebusch, Jon R Lorsch, V Ramakrishnan
Publikováno v:
eLife, Vol 7 (2018)
In eukaryotic translation initiation, AUG recognition of the mRNA requires accommodation of Met-tRNAi in a ‘PIN’ state, which is antagonized by the factor eIF1. eIF5 is a GTPase activating protein (GAP) of eIF2 that additionally promotes stringen
Externí odkaz:
https://doaj.org/article/319843f39d3940a6aee22d48206c5715
Publikováno v:
eLife, Vol 7 (2018)
DEAD-box RNA helicase Ded1 is thought to resolve secondary structures in mRNA 5'-untranslated regions (5'-UTRs) that impede 48S preinitiation complex (PIC) formation at the initiation codon. We reconstituted Ded1 acceleration of 48S PIC assembly on n
Externí odkaz:
https://doaj.org/article/f9b3b39aa6e64d49b72b64614ae65118