Zobrazeno 1 - 10
of 207
pro vyhledávání: '"Alan Engelman"'
Autor:
Alan Engelman, Kenneth A. Matreyek
Publikováno v:
Viruses, Vol 5, Iss 10, Pp 2483-2511 (2013)
Retroviruses integrate their reverse transcribed genomes into host cell chromosomes as an obligate step in virus replication. The nuclear envelope separates the chromosomes from the cell cytoplasm during interphase, and different retroviral groups de
Externí odkaz:
https://doaj.org/article/c3867181449045d8a5886e4c7ba5f593
Autor:
Hao Wang, Ming-Chieh Shun, Xiang Li, Francesca Di Nunzio, Stephen Hare, Peter Cherepanov, Alan Engelman
Publikováno v:
Molecular Therapy: Methods & Clinical Development, Vol 1, Iss C (2014)
Controlling the specificity of retroviral DNA integration could improve the safety of gene therapy vectors, and fusions of heterologous chromatin binding modules to the integrase (IN)–binding domain from the lentiviral integration host cofactor len
Externí odkaz:
https://doaj.org/article/441e4ec4fb3747a0b6bbd81d1d11f540
Publikováno v:
PLoS ONE, Vol 9, Iss 8, p e105078 (2014)
The DNA cutting and joining reactions of HIV-1 integration are catalyzed by integrase (IN), a viral protein that functions as a tetramer bridging the two viral DNA ends (intasome). Two major obstacles for biochemical and structural studies of HIV-1 i
Externí odkaz:
https://doaj.org/article/cc671dd474af4d4ba783265676e37014
Publikováno v:
PLoS ONE, Vol 8, Iss 10, p e76638 (2013)
Integrase is an essential retroviral enzyme, catalyzing the stable integration of reverse transcribed DNA into cellular DNA. Several aspects of the integration mechanism, including the length of host DNA sequence duplication flanking the integrated p
Externí odkaz:
https://doaj.org/article/42318cec863840ffa0acddc12ab65c71
Publikováno v:
PLoS Pathogens, Vol 9, Iss 10, p e1003693 (2013)
Lentiviruses can infect non-dividing cells, and various cellular transport proteins provide crucial functions for lentiviral nuclear entry and integration. We previously showed that the viral capsid (CA) protein mediated the dependency on cellular nu
Externí odkaz:
https://doaj.org/article/35e2d4e6a99d4c6c99a3bec7f47fce89
Autor:
Stephen Hare, Francesca Di Nunzio, Alfred Labeja, Jimin Wang, Alan Engelman, Peter Cherepanov
Publikováno v:
PLoS Pathogens, Vol 5, Iss 7, p e1000515 (2009)
Experimental evidence suggests that a tetramer of integrase (IN) is the protagonist of the concerted strand transfer reaction, whereby both ends of retroviral DNA are inserted into a host cell chromosome. Herein we present two crystal structures cont
Externí odkaz:
https://doaj.org/article/b57452ab680d4d43bf92a1f264e5cdd4
Publikováno v:
PLoS Pathogens, Vol 5, Iss 3, p e1000327 (2009)
Retroviruses and retrotransposons are vulnerable to a suicidal pathway known as autointegration, which occurs when the 3'-ends of the reverse transcript are activated by integrase and then attack sites within the viral DNA. Retroelements have diverse
Externí odkaz:
https://doaj.org/article/bf1303394b94403c9636b95293a676f7
Autor:
Stephen Hare, Ming-Chieh Shun, Saumya Shree Gupta, Eugene Valkov, Alan Engelman, Peter Cherepanov
Publikováno v:
PLoS Pathogens, Vol 5, Iss 1, p e1000259 (2009)
Lens epithelium derived growth factor (LEDGF), also known as PC4 and SFRS1 interacting protein 1 (PSIP1) and transcriptional co-activator p75, is the cellular binding partner of lentiviral integrase (IN) proteins. LEDGF accounts for the characteristi
Externí odkaz:
https://doaj.org/article/f37278f763ce45e297e405e097d7fdef
Autor:
Alan Engelman, Peter Cherepanov
Publikováno v:
PLoS Pathogens, Vol 4, Iss 3, p e1000046 (2008)
Retroviral replication proceeds through a stable proviral DNA intermediate, and numerous host cell factors have been implicated in its formation. In particular, recent results have highlighted an important role for the integrase-interactor lens epith
Externí odkaz:
https://doaj.org/article/39f801444d2f4c69b143385e0bdc0803
Publikováno v:
Nucleic Acids Research
HIV-1 integration favors recurrent integration gene (RIG) targets and genic proviruses can confer cell survival in vivo. However, the relationship between initial RIG integrants and how these evolve in patients over time are unknown. To address these