Zobrazeno 1 - 10
of 27
pro vyhledávání: '"Alaksh, Choudhury"'
Publikováno v:
Nature Communications, Vol 14, Iss 1, Pp 1-16 (2023)
Abstract RNA polymerase (RNAP) is emblematic of complex biological systems that control multiple traits involving trade-offs such as growth versus maintenance. Laboratory evolution has revealed that mutations in RNAP subunits, including RpoB, are fre
Externí odkaz:
https://doaj.org/article/d16934fc926045d6b0a15469f358e7af
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-13 (2020)
Regulatory networks respond to environmental and genetic perturbations by reprogramming metabolism. Here the authors screen a library of 82 regulators with 110,120 mutations to map the regulatory network of 4000 genes.
Externí odkaz:
https://doaj.org/article/600753d8182544abaa6cc9ffdf23ce81
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-9 (2020)
Bacteria lack the same suite of CRISPR tools that have been developed for mammalian cells. Here, the authors link aptamers to sgRNAs to allow small molecule control of gene editing in E. coli.
Externí odkaz:
https://doaj.org/article/ded3f93171cd4f4bb57d5a441ed60ade
Autor:
Cong Chen, Alaksh Choudhury, Shuanghong Zhang, Andrew D. Garst, Xin Song, Xunli Liu, Tao Chen, Ryan T. Gill, Zhiwen Wang
Publikováno v:
mSystems, Vol 5, Iss 2 (2020)
ABSTRACT It is important to expedite our understanding of antibiotic resistance to address the increasing numbers of fatalities and environmental pollution due to the emergence of antibiotic resistance and multidrug-resistant strains. Here, we combin
Externí odkaz:
https://doaj.org/article/8e0568820c9d484880741007f3b5bb0e
Autor:
Alaksh Choudhury, Jacob A Fenster, Reilly G Fankhauser, Joel L Kaar, Olivier Tenaillon, Ryan T Gill
Publikováno v:
Molecular Systems Biology, Vol 16, Iss 3, Pp n/a-n/a (2020)
Abstract Deep mutational scanning can provide significant insights into the function of essential genes in bacteria. Here, we developed a high‐throughput method for mutating essential genes of Escherichia coli in their native genetic context. We us
Externí odkaz:
https://doaj.org/article/5f2e06ad113d433aa65e1e649e871ef6
Publikováno v:
Synthetic and Systems Biotechnology, Vol 1, Iss 1, Pp 2-6 (2016)
Cell-free protein synthesis (CFPS) systems from crude lysates have benefitted from modifications to their enzyme composition. For example, functionally deleting enzymes in the source strain that are deleterious to CFPS can improve protein synthesis y
Externí odkaz:
https://doaj.org/article/5948679f5f4d4035be54a5f6225c725e
Autor:
Xin Song, Yangyang Zheng, Shuting Li, Alaksh Choudhury, Xunli Liu, Tao Chen, Ryan T. Gill, Zhiwen Wang
Publikováno v:
AIChE Journal. 69
Autor:
Ryan T. Gill, Alaksh Choudhury, Reilly G. Fankhauser, Emily F. Freed, Eun Joong Oh, Andrew B. Morgenthaler, Marcelo C. Bassalo, Shelley D. Copley, Joel L. Kaar
Publikováno v:
Choudhury, A, Fankhauser, R G, Freed, E F, Oh, E J, Morgenthaler, A B, Bassalo, M C, Copley, S D, Kaar, J L & Gill, R T 2020, ' Determinants for efficient editing with cas9-mediated recombineering in Escherichia coli ', ACS Synthetic Biology, vol. 9, no. 5, pp. 1083-1099 . https://doi.org/10.1021/acssynbio.9b00440
In E. coli, editing efficiency with Cas9-mediated recombineering varies across targets due to differences in the level of Cas9:gRNA-mediated DNA double-strand break (DSB)-induced cell death. We found that editing efficiency with the same gRNA and rep
Autor:
Khalid J. Alzahrani, Sangram Bagh, Carlos Barreiro, Upasana Basu, Gargi Bhattacharjee, Juan Luis Blazquez-Roman, Logan R. Burrington, Pablo Carbonell, Nils Chapin, Numaan Cheema, Alaksh Choudhury, Laila A. Damiati, Safa A. Damiati, Samar Damiati, Shalini S. Deb, Archit Devarajan, Debarun Dhali, Gurneet K. Dhanoa, Carrie A. Eckert, Francisco J. Enguita, Tamás Fehér, Jake Flood, Emily F. Freed, Ashok Ganesan, Carlos García-Estrada, Seyedkhashayar Ghavami, Nisarg Gohil, Dhwani Gupta, Margaret Habib, Nedim Hacıosmanoğlu, Susu He, Seok Hoon Hong, Ali Yazdizadeh Kharrazi, Boris Kirov, İlkay Çisil Köksaldi, Sıla Köse, Shashi Kumar, Srinivasan Ganesh Kumar, Büşra Merve Kırpat, Maria Priscila Lacerda, Navya L. Lam, Ana Lúcia Leitão, Liya Liang, Rongming Liu, Indra Mani, Rupesh Maurya, Georgi Y. Miloshev, Kushika Mitra, Nihal Mohammed, Christian Møller-Olsen, Julian Ostaku, Lozena A. Otcheva, Javin P. Oza, Gökçe Özkul, Shilpa Pal, Amir Pandi, Dimitris Papamichail, Georgios Papamichail, Subha Sankar Paul, Nikolet Pavlova, Robert Penchovsky, Slavil Peykov, Mara Pisani, Katya B. Popova, Shamlan M.S. Reshamwala, Antonia P. Sagona, Behide Saltepe, Kathakali Sarkar, Ana Serratosa Fernandez-Baca, Ashima Sharma, Velia Siciliano, Mansoor A. Siddiqui, Vijai Singh, Kevin Smith, Heykel Trabelsi, Martina Traykovska, Aikaterini Valsamatzi-Panagiotou, Elizabeth J. Vojvoda, Dong-Dong Yang, Yazen Yaseen, Merve Yavuz, Syed Shams Yazdani, Amir Zare, Urartu Özgür Şafak Şeker
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::734db77d195e15077c8dde45c0ed1c85
https://doi.org/10.1016/b978-0-12-824469-2.00038-5
https://doi.org/10.1016/b978-0-12-824469-2.00038-5