Zobrazeno 1 - 10
of 36
pro vyhledávání: '"Alakhov VYu"'
Publikováno v:
Pharmaceutical research. 15(6)
The present work characterizes the effects of Pluronic copolymers on the transport of a P-gp-dependent probe, rhodamine 123 (R123) in Caco-2 cell monolayers.The accumulation and efflux studies were performed on the confluent Caco-2 monolayers using f
Autor:
Av, Kabanov, Vi, Slepnev, Le, Kuznetsova, Ev, Batrakova, Alakhov VYu, Ns, Melik-Nubarov, Peter Sveshnikov, Va, Kabanov
Publikováno v:
Europe PubMed Central
Micelles of pluronic P85 (poly(oxyethylene)-poly(oxypropylene) block copolymer) are used as microcontainers for in vitro delivery of fluorescein into Jurkat and MDCK cells. In order to target the fluorescein containing micelles into the cell, Staphyl
Publikováno v:
Biochemistry international. 26(4)
Pluronic P85 (poly(oxyethylene)-poly(oxypropylene) block copolymer) was used for in vitro delivery of [gamma-32P]ATP into intact Jurkat cells. Negatively charged ATP molecules are not able to penetrate cell plasma membrane. Hence, exogenous [gamma-32
Autor:
V I, Tarasov, Alakhov VYu
Publikováno v:
Biochemistry international. 25(5)
Theoretical analysis of the primary amino acid structure of St. aureus enterotoxin B for predicting some structure-functional characteristics is presented. The method is based on correlation between the spectrum of numerical presentation of any amino
Publikováno v:
Biotechnology and applied biochemistry. 12(1)
In order to impart an ability for receptor-independent transmembrane transfer to water-soluble proteins, it has been suggested that they be hydrophobized by lipid groups (fatty acids, etc.). To this end, systems of reversed micelles of surfactants in
Autor:
Alakhov VYu, S A, Arzhakov, O V, Vasilenko, S G, Voloshchuk, I S, Glazkova-Stepanenko, I A, Duvakin, A V, Kabanov, V A, Kabanov, Klinskii EYu, T N, Kravtsova
Publikováno v:
Biomedical science. 1(2)
A new technique for the creation of compounds with targeted action is described. In such compounds, termed respecrins (receptor-specific, screened toxin), a physiologically active component carries an epitope-containing fragment of the target antigen
Publikováno v:
Biomedical science. 1(1)
An effective method for the artificial attachment of lipid anchors to water-soluble proteins has been developed. Protein molecules are modified by a water-insoluble reagent, e.g. fatty acid chloride, in a reversed-micelle system. The resulting fatty
Autor:
A V, Kabanov, A V, Ovcharenko, N S, Melik-Nubarov, A I, Bannikov, T P, Lisok, E V, Klyushnenkova, N G, Cherchenko, Alakhov VYu, A V, Levashov, V I, Kiselev
Publikováno v:
Biomedical science. 1(1)
A method is proposed for the inhibition of viral reproduction in cells by means of fatty-acylated antiviral antibodies which, in contrast to the unmodified antibodies, have the ability to enter the cells. The potential of this technique is demonstrat
Publikováno v:
Biotechnology and applied biochemistry. 10(4)
Monoclonal antibodies against human erythrocyte membrane Ca2+-ATPase were obtained. The binding of monoclonal antibodies to the enzyme resulted in a decrease in the enzyme sensitivity to calmodulin (CaM). The effects of monoclonal antibodies on other
Autor:
Alakhov VYu, Moskaleva EYu, T N, Kravtzova, V V, Smirnov, I A, Duvakin, B V, Loginov, E S, Severin
Publikováno v:
Biotechnology and applied biochemistry. 10(6)
The effects of Staphylococcus aureus enterotoxin A (SEA) on proliferative activities of human peripheral blood lymphocytes, B-lymphoma cells of the Namalva line, and nerve cells of the PC12 line have been studied. It has been shown that SEA affects t