Dual-action CXCR4-targeting liposomes in leukemia: function blocking and drug delivery

Autor: Catriona McCallion, Anna D. Peters, Andrew Booth, Karen Rees-Unwin, Julie Adams, Raisa Rahi, Alain Pluen, Claire V. Hutchinson, Simon J. Webb, John Burthem

Publikováno v: Blood Advances, Vol 3, Iss 14, Pp 2069-2081 (2019)

Abstract: CXC chemokine receptor 4 (CXCR4) is overexpressed by a broad range of hematological disorders, and its interaction with CXC chemokine ligand 12 (CXCL12) is of central importance in the retention and chemoprotection of neoplastic cells in th

Externí odkaz: https://doaj.org/article/2507d194e7a244fc8e6f2e80527f29d0

Chemometrics in Protein Formulation: Stability Governed by Repulsion and Protein Unfolding

Autor: Alina Kulakova, Dillen Augustijn, Inas El Bialy, Lorenzo Gentiluomo, Maria Laura Greco, Stefan Hervø-Hansen, Sowmya Indrakumar, Sujata Mahapatra, Marcello Martinez Morales, Christin Pohl, Marco Polimeni, Aisling Roche, Hristo L. Svilenov, Andreas Tosstorff, Matja Zalar, Robin Curtis, Jeremy P. Derrick, Wolfgang Frieß, Alexander P. Golovanov, Mikael Lund, Allan Nørgaard, Tarik A. Khan, Günther H. J. Peters, Alain Pluen, Dierk Roessner, Werner W. Streicher, Christopher F. van der Walle, Jim Warwicker, Shahid Uddin, Gerhard Winter, Jens Thostrup Bukrinski, Åsmund Rinnan, Pernille Harris

Publikováno v: Molecular Pharmaceutics.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::b30e33ef72e37b5def8fabb536c102a3
https://doi.org/10.1021/acs.molpharmaceut.3c00013

Micro- and macro-viscosity relations in high concentration antibody solutions

Autor: Robin Curtis, Maryam Shah, Shahid Uddin, Peter Davis, Daniel Corbett, C. F. van der Walle, Aisling Roche, Nicole Sibanda, Alain Pluen, Alfredo Lanzaro

Publikováno v: Shah, M, Corbett, D, Lanzaro, A, Roche, A, Sibanda, N, Davis, P, Uddin, S, van der Walle, C F, Curtis, R & Pluen, A 2020, ' Micro-and macro-viscosity relations in high concentration antibody solutions ', European Journal of Pharmaceutics and Biopharmaceutics, vol. 153, 10.1016/j.ejpb.2020.06.007, pp. 211-221 . https://doi.org/10.1016/j.ejpb.2020.06.007

Molecular crowding in highly concentrated monoclonal antibody (mAb) solutions results in significant increases in viscosity, which complicates fill-finish steps and patient administration by subcutaneous injection. As viscosity measurements for optim

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::44af34c70e2efb699240eabf69a2d8d4
https://doi.org/10.1016/j.ejpb.2020.06.007

Arginine to Lysine Mutations Increase the Aggregation Stability of a Single-Chain Variable Fragment through Unfolded-State Interactions

Autor: Angela Thistlethwaite, Jim Warwicker, Karl Fisher, Reza Esfandiary, Alexander P. Golovanov, James Austerberry, Alain Pluen, Jeremy P. Derrick, Robin Curtis, Christopher F. van der Walle

Publikováno v: Austerberry, J, Thistlethwaite, A, Fisher, K, Golovanov, A P, Pluen, A, Esfandiary, R, van der Walle, C F, Warwicker, J, Derrick, J P & Curtis, R 2019, ' Arginine to lysine mutations increase the aggregation stability of a single chain variable fragment through unfolded state interactions ', Biochemistry . https://doi.org/10.1021/acs.biochem.9b00367

Increased protein solubility is known to correlate with an increase in the proportion of lysine over arginine residues. Previous work has shown that the aggregation propensity of a single-chain variable fragment (scFv) does not correlate with its con

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d277cd4cf8550ca893b6546be21832d3
https://doi.org/10.1021/acs.biochem.9b00367

Advancing Therapeutic Protein Discovery and Development through Comprehensive Computational and Biophysical Characterization

Autor: Shahid Uddin, Christin Pohl, Christopher F. van der Walle, Inas El Bialy, Jeremy P. Derrick, Andreas Tosstorff, Robin Curtis, Sujata Mahapatra, Werner Streicher, Dillen Augustijn, Aisling Roche, Alina Kulakova, Wolfgang Frieß, Åsmund Rinnan, Hristo L. Svilenov, Dierk Roessner, Lorenzo Gentiluomo, Sowmya Indrakumar, Alain Pluen, Gerhard Winter, Pernille Harris, Allan Nørgaard, Günther H.J. Peters, Maria Laura Greco, Marcello Martinez Morales, Tarik A. Khan

Publikováno v: Molecular Pharmaceutics
Gentiluomo, L, Svilenov, H L, Augustijn, D, El Bialy, I, Greco, M L, Vitaliyivna Kulakova, A, Indrakumar, S, Mahapatra, S, Morales, M M, Pohl, C, Roche, A, Tosstorff, A, Curtis, R, Derrick, J P, Nørgaard, A, Khan, T A, Peters, G H J, Pluen, A, Rinnan, Å, Streicher, W W, van der Walle, C F, Uddin, S, Winter, G, Roessner, D, Harris, P & Frieß, W 2020, ' Advancing Therapeutic Protein Discovery and Development through Comprehensive Computational and Biophysical Characterization ', Molecular Pharmaceutics, vol. 17, no. 2, pp. 426-440 . https://doi.org/10.1021/acs.molpharmaceut.9b00852
Gentiluomo, L, Svilenov, H L, Augustijn, D, El Bialy, I, Greco, M L, Kulakova, A, Indrakumar, S, Mahapatra, S, Morales, M M, Pohl, C, Roche, A, Tosstorff, A, Curtis, R, Derrick, J P, Nørgaard, A, Khan, T A, Peters, G H J, Pluen, A, Rinnan, Å, Streicher, W W, van der Walle, C F, Uddin, S, Winter, G, Roessner, D, Harris, P & Friess, W 2020, ' Advancing Therapeutic Protein Discovery and Development through Comprehensive Computational and Biophysical Characterization ', Molecular Pharmaceutics, vol. 17, no. 2, pp. 426-440 . https://doi.org/10.1021/acs.molpharmaceut.9b00852

Therapeutic protein candidates should exhibit favorable properties that render them suitable to become drugs. Nevertheless, there are no well-established guidelines for the efficient selection of proteinaceous molecules with desired features during e

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::414939ada22b81281c984e3f808bda3d