Long Open Reading Frame Transcripts Escape Nonsense-Mediated mRNA Decay in Yeast

Autor: Laurence Decourty, Antonia Doyen, Christophe Malabat, Emmanuel Frachon, Delphine Rispal, Bertrand Séraphin, Frank Feuerbach, Alain Jacquier, Cosmin Saveanu

Publikováno v: Cell Reports, Vol 6, Iss 4, Pp 593-598 (2014)

Nonsense-mediated mRNA decay (NMD) destabilizes eukaryotic transcripts with long 3′ UTRs. To investigate whether other transcript features affect NMD, we generated yeast strains expressing chromosomal-derived mRNAs with 979 different promoter and o

Externí odkaz: https://doaj.org/article/7e77ba57b9334124ad7b0f22e19c7d82

A SKI subcomplex specifically required for the degradation of ribosome-free RNA regions

Autor: Micheline Fromont-Racine, Abdelkader Namane, Elodie Zhang, Antonia Doyen, Estelle Dacheux, Varun Khanna, Bernard Turcotte, Alain Jacquier

SummaryThe Ski2-Ski3-Ski8 (SKI) complex assists the RNA exosome during the 3’-5’ degradation of cytoplasmic transcripts. Previous reports showed that the SKI complex is involved in the 3’-5’ degradation of mRNA, including 3’ untranslated re

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a16b8567e83356919d9fb06737330c86
https://hal-pasteur.archives-ouvertes.fr/pasteur-03440988

Investigation of RNA metabolism through large-scale genetic interaction profiling in yeast

Autor: Laurence, Decourty, Christophe, Malabat, Emmanuel, Frachon, Alain, Jacquier, Cosmin, Saveanu

Publikováno v: Nucleic Acids Research
Nucleic Acids Research, Oxford University Press, 2021, pp.8535-8555. ⟨10.1093/nar/gkab680⟩
Nucleic Acids Research, 2021, pp.8535-8555. ⟨10.1093/nar/gkab680⟩

International audience; Abstract Gene deletion and gene expression alteration can lead to growth defects that are amplified or reduced when a second mutation is present in the same cells. We performed 154 genetic interaction mapping (GIM) screens wit

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::cc1f28b2fa7d8627b12aa2fd97168446
https://hal.archives-ouvertes.fr/hal-03320796

Sodium selenide toxicity is mediated by O2-dependent DNA breaks.

Autor: Gérald Peyroche, Cosmin Saveanu, Marc Dauplais, Myriam Lazard, François Beuneu, Laurence Decourty, Christophe Malabat, Alain Jacquier, Sylvain Blanquet, Pierre Plateau

Publikováno v: PLoS ONE, Vol 7, Iss 5, p e36343 (2012)

Hydrogen selenide is a recurrent metabolite of selenium compounds. However, few experiments studied the direct link between this toxic agent and cell death. To address this question, we first screened a systematic collection of Saccharomyces cerevisi

Externí odkaz: https://doaj.org/article/e9c6b80a22464d87a2bdee148b2d7bd3

The ribosome-bound quality control complex remains associated to aberrant peptides during their proteasomal targeting and interacts with Tom1 to limit protein aggregation

Autor: Quentin Defenouillère, Micheline Fromont-Racine, Abdelkader Namane, Alain Jacquier, John Mouaikel

Publikováno v: Molecular Biology of the Cell
Molecular Biology of the Cell, American Society for Cell Biology, 2017, 28 (9), pp.1165-1176. ⟨10.1091/mbc.E16-10-0746⟩
Molecular Biology of the Cell, 2017, 28 (9), pp.1165-1176. ⟨10.1091/mbc.E16-10-0746⟩

The RQC complex involved in protein quality control mechanisms also exists as a ribosome-unbound complex during the escort of aberrant peptides to the proteasome. The E3 ubiquitin ligase Tom1 is a newly identified partner of this light version of the

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2eb1775dc1110973a7aba71713bc78ca
https://doi.org/10.1091/mbc.e16-10-0746