Zobrazeno 1 - 6
of 6
pro vyhledávání: '"Akshat Mullerpatan"'
Autor:
Henrik Andersen, André Nascimento, Pankaj Karande, Erin Kane, Akshat Mullerpatan, Ronit Ghosh, Steven M. Cramer
Publikováno v:
Biotechnology and Bioengineering. 117:3775-3784
Affinity precipitation using stimulus-responsive biopolymers such as elastin-like polypeptides (ELPs) have been successfully employed for the purification of monoclonal antibodies. In the current work, we extend these studies to the development of an
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ed42286418fa0e1846682e92184c9260
https://doi.org/10.1002/bit.27539
https://doi.org/10.1002/bit.27539
Autor:
John P. Trasatti, Nicholas Vecchiarello, Chaz Goodwine, Pankaj Karande, Steven M. Timmick, Akshat Mullerpatan, Divya G. Shastry, Steven M. Cramer, Divya Chandra
Publikováno v:
Journal of Chemical Technology & Biotechnology. 94:2345-2354
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::53146d2ec3723f147eb5dba1240006cb
https://doi.org/10.1002/jctb.6029
https://doi.org/10.1002/jctb.6029
Autor:
Sanchayita Ghose, Jie Chen, Steven M. Cramer, Akshat Mullerpatan, Zheng Jian Li, Manish Bhat, Melissa Holstein
Publikováno v:
Journal of biotechnology. 338
This paper describes a simplified affinity precipitation process for the purification of mAbs from complex mixtures using elastin-like polypeptide fused to a single Z domain of protein A (ELP-Z). This approach eliminates several steps in the original
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5fb4b71485a3602aaf4aedc30f3dd836
https://pubmed.ncbi.nlm.nih.gov/34197822
https://pubmed.ncbi.nlm.nih.gov/34197822
Autor:
André Nascimento, Steven M. Cramer, Erin Kane, Akshat Mullerpatan, Pankaj Karande, Ronit Ghosh, Henrik Andersen
Affinity precipitation using stimulus-responsive biopolymers such as Elastin-like Polypeptides (ELPs) have been successfully employed for the purification of monoclonal antibodies. In the current work, we extend these studies to the development of an
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::7ba103744ea60473c95c47b7aacfe562
https://doi.org/10.22541/au.158880246.67448980
https://doi.org/10.22541/au.158880246.67448980
Publikováno v:
Journal of biotechnology. 309
In this work, we employed fusions of affinity peptides and elastin-like polypeptide (ELP) to carry out a proof-of-concept study for the single-step purification of model, tag-free proteins. Three known peptide-protein binding pairs of varied binding
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::22ca01b0a082976d6885937ae55a6bf4
https://pubmed.ncbi.nlm.nih.gov/31874207
https://pubmed.ncbi.nlm.nih.gov/31874207
Autor:
Pankaj Karande, Ana Margarida Azevedo, André Nascimento, Steven M. Cramer, Akshat Mullerpatan
Publikováno v:
Biotechnology progressREFERENCES. 35(6)
In this work, two phage biopanning strategies were developed to identify affinity peptides for a single Fab and multiple kappa Fabs. For the biopanning rounds, protein L beads were employed to bind Fab targets in a fixed orientation, and NHS function
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7ef54b07933b41c195cfdd9c10e0965d
https://pubmed.ncbi.nlm.nih.gov/31301216
https://pubmed.ncbi.nlm.nih.gov/31301216