Computer-aided engineering of a branching sucrase for the glucodiversification of a tetrasaccharide precursor of S. flexneri antigenic oligosaccharides

Autor: Mounir Benkoulouche, Akli Ben Imeddourene, Louis-Antoine Barel, Dorian Lefebvre, Mathieu Fanuel, Hélène Rogniaux, David Ropartz, Sophie Barbe, David Guieysse, Laurence A. Mulard, Magali Remaud-Siméon, Claire Moulis, Isabelle André

Publikováno v: Scientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)

Abstract Enzyme engineering approaches have allowed to extend the collection of enzymatic tools available for synthetic purposes. However, controlling the regioselectivity of the reaction remains challenging, in particular when dealing with carbohydr

Externí odkaz: https://doaj.org/article/6fca7743f5704259be614afb146d57e5

Combining multi-scale modelling methods to decipher molecular motions of a branching sucrase from glycoside-hydrolase family 70.

Autor: Akli Ben Imeddourene, Jérémy Esque, Isabelle André

Publikováno v: PLoS ONE, Vol 13, Iss 8, p e0201323 (2018)

Among α-transglucosylases from Glycoside-Hydrolase family 70, the ΔN123-GB-CD2 enzyme derived from the bifunctional DSR-E from L. citreum NRRL B-1299 is particularly interesting as it was the first described engineered Branching Sucrase, not able t

Externí odkaz: https://doaj.org/article/9b0a56a25c1c4a1ba1c99dea99f29f49

A convergent chemoenzymatic strategy to deliver a diversity of Shigella flexneri serotype-specific O-antigen segments from a unique lightly protected tetrasaccharide core

Autor: Akli Ben Imeddourene, Guillaume Le Heiget, Claire Moulis, Isabelle André, Zhaoyu Hu, Nelly Monties, Laurence A. Mulard, Magali Remaud-Simeon, Mounir Benkoulouche, Louis-Antoine Barel, Catherine Guerreiro, Yann Le Guen

Publikováno v: Journal of Organic Chemistry
Journal of Organic Chemistry, 2021, 86 (3), pp.2058-2075. ⟨10.1021/acs.joc.0c00777⟩
Journal of Organic Chemistry, American Chemical Society, 2020, 86 (3), pp.2058-2075. ⟨10.1021/acs.joc.0c00777⟩

International audience; Progress in glycoscience is strongly dependent on the availability of broadly diverse tailored-made, well-defined and often complex oligosaccharides. Herein, going beyond natural resources and aiming to circumvent chemical bou

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d9424a376996bb908628ac2d5c4f976e
https://hal.insa-toulouse.fr/hal-03020947/document

The intrinsic mechanics of B-DNA in solution characterized by NMR

Autor: Akli Ben Imeddourene, Xiaoqian Xu, Christophe Oguey, Brigitte Hartmann, Loussiné Zargarian, Nicolas Foloppe, Olivier Mauffret

Publikováno v: Nucleic Acids Research
Nucleic Acids Research, Oxford University Press, 2016, 44 (7), pp.3432-3447. ⟨10.1093/nar/gkw084⟩
Nucleic Acids Research, 2016, 44 (7), pp.3432-3447. ⟨10.1093/nar/gkw084⟩

International audience; Experimental characterization of the structural couplings in free B-DNA in solution has been elusive, because of subtle effects that are challenging to tackle. Here, the exploitation of the NMR measurements collected on four d

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::31068f6be868b397b09a014589517dce
http://europepmc.org/articles/PMC4838374

Structural and Dynamical Impact of a Universal Fluorescent Nucleoside Analogue Inserted Into a DNA Duplex

Autor: Nelly Morellet, Loussiné Zargarian, Olivier Mauffret, Yves Mély, Brigitte René, Nicolas Barthes, Krishna Gavvala, Alain Burger, Akli Ben Imeddourene, Benoît Y. Michel, Philippe Fosse, Cyril A. Kenfack

Publikováno v: Journal of Physical Chemistry B
Journal of Physical Chemistry B, American Chemical Society, 2017, 121 (50), pp.11249-11261. ⟨10.1021/acs.jpcb.7b08825⟩
Journal of Physical Chemistry B, 2017, 121 (50), pp.11249-11261. ⟨10.1021/acs.jpcb.7b08825⟩

Recently, a 3-hydroxychromone based nucleoside 3HCnt has been developed as a highly environment-sensitive nucleoside surrogate to investigate protein-DNA interactions. When it is incorporated in DNA, the probe is up to 50-fold brighter than 2-aminopu

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6d4e8c048af6cbb96e1c329c68cfcb6c
https://hal.archives-ouvertes.fr/hal-02119010