Zobrazeno 1 - 10
of 136
pro vyhledávání: '"Akitsu, Hotta"'
Publikováno v:
Scientific Reports, Vol 14, Iss 1, Pp 1-12 (2024)
Abstract The forced expression system of MYOD1, a master gene for myogenic differentiation, can efficiently and rapidly reproduce muscle differentiation of human induced pluripotent stem cells (hiPSCs). Despite these advantages of the MYOD1 overexpre
Externí odkaz:
https://doaj.org/article/0288be546cf347c9a9fab5b940690d3e
Publikováno v:
Stem Cell Research & Therapy, Vol 15, Iss 1, Pp 1-14 (2024)
Abstract Background Fibrodysplasia ossificans progressiva (FOP) is a rare genetic disease caused by a gain-of-function mutation in ACVR1, which is a bone morphogenetic protein (BMP) type I receptor. Moreover, it causes progressive heterotopic ossific
Externí odkaz:
https://doaj.org/article/6a24b948d17942799a13d0d29082b2e3
Autor:
Misato Nakanishi-Koakutsu, Kenji Miki, Yuki Naka, Masako Sasaki, Takayuki Wakimizu, Stephanie C. Napier, Chikako Okubo, Megumi Narita, Misato Nishikawa, Reo Hata, Kazuhisa Chonabayashi, Akitsu Hotta, Kenichi Imahashi, Tomoyuki Nishimoto, Yoshinori Yoshida
Publikováno v:
Communications Biology, Vol 7, Iss 1, Pp 1-14 (2024)
Abstract Current differentiation protocols for human induced pluripotent stem cells (hiPSCs) produce heterogeneous cardiomyocytes (CMs). Although chamber-specific CM selection using cell surface antigens enhances biomedical applications, a cell surfa
Externí odkaz:
https://doaj.org/article/73e8538871d040e3ae7023f2f88a2a49
Autor:
Ryuichiro Hirayama, Kosuke Toyohara, Kei Watanabe, Takeya Otsuki, Toshikazu Araoka, Shin-Ichi Mae, Tomoko Horinouchi, Tomohiko Yamamura, Keisuke Okita, Akitsu Hotta, Kazumoto Iijima, Kandai Nozu, Kenji Osafune
Publikováno v:
Communications Biology, Vol 6, Iss 1, Pp 1-11 (2023)
Abstract Alport syndrome (AS) is a hereditary glomerulonephritis caused by COL4A3, COL4A4 or COL4A5 gene mutations and characterized by abnormalities of glomerular basement membranes (GBMs). Due to a lack of curative treatments, the condition proceed
Externí odkaz:
https://doaj.org/article/d35615f2c8824439a7a88aa52196dc4a
Publikováno v:
Scientific Reports, Vol 13, Iss 1, Pp 1-17 (2023)
Abstract Myotonic dystrophy type 1 (DM1) is caused by expanded CTG repeats (CTGexp) in the dystrophia myotonica protein kinase (DMPK) gene, and the transcription products, expanded CUG repeats, sequester muscleblind like splicing regulator 1 (MBNL1),
Externí odkaz:
https://doaj.org/article/fa71afad8748492e95365ac375fec8b3
Autor:
Yuko Kitano, Sayaka Nishimura, Tomoaki M. Kato, Anna Ueda, Kaho Takigawa, Masafumi Umekage, Masaki Nomura, Ayane Kawakami, Haruna Ogawa, Huaigeng Xu, Akitsu Hotta, Naoko Takasu, Masayoshi Tsukahara
Publikováno v:
Molecular Therapy: Methods & Clinical Development, Vol 26, Iss , Pp 15-25 (2022)
In order to expand the promise of regenerative medicine using allogeneic induced pluripotent stem cells (iPSCs), precise and efficient genome editing of human leukocyte antigen (HLA) genes would be advantageous to minimize the immune rejection caused
Externí odkaz:
https://doaj.org/article/94c9d417429446f8b649414f61abd42a
Autor:
Rina Hashimoto, Ayaka Sakamoto, Sayaka Deguchi, Renxing Yi, Emi Sano, Akitsu Hotta, Kazutoshi Takahashi, Shinya Yamanaka, Kazuo Takayama
Publikováno v:
Molecular Therapy: Nucleic Acids, Vol 26, Iss , Pp 1107-1114 (2021)
It has been reported that many receptors and proteases are required for severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection. Although angiotensin-converting enzyme 2 (ACE2) is the most important of these receptors, little is known
Externí odkaz:
https://doaj.org/article/261a1d9f45ae40909ca57488eb8c5535
Autor:
Eriya Kenjo, Hiroyuki Hozumi, Yukimasa Makita, Kumiko A. Iwabuchi, Naoko Fujimoto, Satoru Matsumoto, Maya Kimura, Yuichiro Amano, Masataka Ifuku, Youichi Naoe, Naoto Inukai, Akitsu Hotta
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-13 (2021)
In vivo delivery of CRISPR-Cas9 holds promise for treating muscular dystrophy, however, AAV delivery is known to be immunogenic. Here, the authors show that LNP delivery of CRISPR-Cas9 enables repeated injections into skeletal muscle and leads to res
Externí odkaz:
https://doaj.org/article/82ba1424688643b8923145dd7be8e610
Autor:
Masashi Noda, Kohei Tatsumi, Hideto Matsui, Yasunori Matsunari, Takeshi Sato, Yasushi Fukuoka, Akitsu Hotta, Teruo Okano, Kimihiko Kichikawa, Mitsuhiko Sugimoto, Midori Shima, Kenji Nishio
Publikováno v:
Regenerative Therapy, Vol 18, Iss , Pp 347-354 (2021)
Introduction: Gene therapy have recently attracted much attention as a curative therapeutic option for inherited single gene disorders such as hemophilia. Hemophilia is a hereditary bleeding disorder caused by the deficiency of clotting activity of f
Externí odkaz:
https://doaj.org/article/a3e5595250fe4d9daf624a5260659797
Autor:
Peter Gee, Mandy S. Y. Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F. Yang, Mio Iwasaki, Xiou H. Wang, Matthew A. Waller, Nanako Shirai, Yasuko O. Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita, Kumiko A. Iwabuchi, Masahiko Yasuda, Huaigeng Xu, Takeshi Noda, Jun Komano, Hidetoshi Sakurai, Naoto Inukai, Akitsu Hotta
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-18 (2020)
Expression of Cas9 and gRNA from viral vectors in vivo may cause off-target activity. Here the authors present NanoMEDIC, which uses nanovesicles to transiently deliver editing machinery to hard-to-transfect cells.
Externí odkaz:
https://doaj.org/article/4d0fe2ef9a604f0fb6865d1e08b1ed50