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Insights into phosphatase-activated chemical defense in a marine sponge holobiont

Autor: Yoko Egami, Ikuro Abe, Kenichi Matsuda, Toshiyuki Wakimoto, Takahiro Jomori, Akira Takai

Publikováno v: RSC Chemical Biology

Marine sponges often contain potent cytotoxic compounds, which in turn evokes the principle question of how marine sponges avoid self-toxicity. In a marine sponge Discodermia calyx, the highly toxic calyculin A is detoxified by the phosphorylation, w

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::29aea1ce79b3e05bd8e5b8cdc779ff82
https://doi.org/10.1039/d1cb00163a

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An improved fluorescent protein-based expression reporter system that utilizes bioluminescence resonance energy transfer and peptide-assisted complementation

Autor: Keiko Yoshizawa, Yasushi Okada, Tomonobu M. Watanabe, Taishi Kakizuka, Akira Takai

Publikováno v: Chemical Communications. 56:3625-3628

Fluorescent protein-based reporter systems are used to track gene expression in cells. Here, we propose a modified bioluminescence resonance energy transfer (BRET) reporter as a maturation-less reporter that utilizes a peptide-assisted complementatio

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::296c13e75a9e0950b0b7aff9c23642c2
https://doi.org/10.1039/c9cc08664a

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Addition of urea and thiourea to electrophoresis sample buffer improves efficiency of protein extraction from TCA/acetone-treated smooth muscle tissues for phos-tag SDS-PAGE

Autor: Akira Takai, Toshiyuki Kaneko, Kosuke Takeya, Motoi Miyazu

Publikováno v: ELECTROPHORESIS. 39:326-333

Phosphorylation analysis by using phos-tag technique has been reported to be suitable for highly sensitive quantification of smooth muscle myosin regulatory light chain (LC20) phosphorylation. However, there is another factor that will affect the sen

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ddda4523b02364aff73c9e2608bc57dc
https://doi.org/10.1002/elps.201700394

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Protein phosphatases 1 and 2A and their naturally occurring inhibitors: current topics in smooth muscle physiology and chemical biology

Autor: Masumi Eto, Masaru Watanabe, Akira Takai, Katsuya Hirano, Toshiyuki Wakimoto, Kosuke Takeya

Publikováno v: The Journal of Physiological Sciences

Protein phosphatases 1 and 2A (PP1 and PP2A) are the most ubiquitous and abundant serine/threonine phosphatases in eukaryotic cells. They play fundamental roles in the regulation of various cellular functions. This review focuses on recent advances i

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c4e621fc21fb567fc17e53c318b481a8
https://doi.org/10.1007/s12576-017-0556-6

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Urinary sex steroid hormone and placental leucine aminopeptidase concentration differences between live births and stillbirth of Bornean orangutans (Pongo pygmaeus)

Autor: Toshio Kobayashi, Miho Inoue-Murayama, Gen'ichi Idani, Akihiro Ouchi, Yusuke Sano, Yasuhiko Ozaki, Kodzue Kinoshita, Noko Kuze, Mika Shimizu, Munehiro Okamoto, Akira Takai

Publikováno v: Journal of Medical Primatology. 46:3-8

Background Under the environment of pregnancy, the placenta assumes an important steroidogenic role in the maintenance of pregnancy. Methods Urinary placental leucine aminopeptidase (PLAP), estrone-3-glucuronide (E1G), and pregnanediol-3-glucuronide

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b1c79197d18de721ae564d9fb63f5d41
https://doi.org/10.1111/jmp.12249

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