Zobrazeno 1 - 10
of 90
pro vyhledávání: '"Akio Sugihara"'
In this chapter, the authors describe the gene cloning, structures, and production mechanisms of microbial lipases and their production by recombinant microbes. The total RNA was extracted with 4 M guanidine thiocyanate solution from log-phase cells
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c8a3aa205a9c5a0017f32b6dadc52d0a
https://doi.org/10.1201/9781003067191-23
https://doi.org/10.1201/9781003067191-23
Autor:
Susumu Tsunasawa, Akio Sugihara, Takayuki Mizuno, Shigeo Sugihara, Yoshinori Hirota, Daisuke Nakayama, Masanori Bunya, Atsushi Maeda
Publikováno v:
Journal of Bioscience and Bioengineering. 105:341-349
A novel cholesterol esterase with there and throughout to synthesisze steryl ester was obtained from the culture filtrate of a fungal strain Trichoderma sp. AS59 isolated from soil. The extracellular enzyme was a monomeric protein with a molecular ma
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::467db4a05ef1695b57bfe49ea68b6036
https://doi.org/10.1263/jbb.105.341
https://doi.org/10.1263/jbb.105.341
Publikováno v:
Biochemical Engineering Journal. 27:72-76
Saccharomyces cerevisiae has the potential for morphological differentiation. In order to positively utilize the morphological transition of S. cerevisiae for the separation of cells from culture broth, the cell settling of mutant J19 having pseudohy
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b9c4f28604c98596c36ab684e1b05c3a
https://doi.org/10.1016/j.bej.2005.07.001
https://doi.org/10.1016/j.bej.2005.07.001
Autor:
Praphan Pinsirodom, Akio Sugihara, Takashi Kobayashi, Toshihiro Nagao, Yomi Watanabe, Yuji Shimada
Publikováno v:
Journal of the American Oil Chemists' Society. 81:543-547
Production of MAG by a lipase-catalyzed reaction is known to be effective at low temperature. This phenomenon can be explained by assuming that synthesized MAG are excluded from the reaction system because MAG, which have low m.p., are solidified at
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b4ff0eceb4e3956753bd04901c810cfd
https://doi.org/10.1007/s11746-006-0938-z
https://doi.org/10.1007/s11746-006-0938-z
Autor:
Yuji Shimada, Akiko Kawashima, Harukazu Fukami, Motoo Sumida, Toshihiro Nagao, Kengo Akimoto, Akio Sugihara, Yomi Watanabe
Publikováno v:
Journal of the American Oil Chemists' Society. 80:867-872
Two oils containing a large amount of 2-arachidonoyl-TAG were selected to produce structured TAG rich in 1,3-capryloyl-2-arachidonoyl glycerol (CAC). An oil (TGA58F oil) was prepared by fermentation of Mortierella alpina, in which the 2-arachidonyoyl
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b49eaabdf0ef2c88e4af4371a0570f16
https://doi.org/10.1007/s11746-003-0787-9
https://doi.org/10.1007/s11746-003-0787-9
Autor:
Kentaro Tsutsumi, Toshihiro Nagao, Akio Sugihara, Yoshie Yamauchi-Sato, Takaya Yamamoto, Yuji Shimada, Yomi Watanabe
Publikováno v:
Journal of the American Oil Chemists' Society. 80:909-914
We attempted to produce MAG of CLA through lipase-catalyzed esterification of a FFA mixture containing CLA (referred to as FFA-CLA) with glycerol. Screening of lipases showed that MAG-CLA was produced efficiently at 5°C with Penicillium camembertii,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ecf68b07fa8654ad33b4fcc08387e42f
https://doi.org/10.1007/s11746-003-0795-9
https://doi.org/10.1007/s11746-003-0795-9
Autor:
Yuji Shimada, Yoshinori Hirota, Aktohiro Kitano, Yomi Watanabe, Masaharu Suenaga, Akio Sugihara, Toshihiro Nagao, Seiichi Nakai
Publikováno v:
Journal of the American Oil Chemists' Society. 80:341-346
Soybean oil deodorizer distillate (SODD) contains steryl esters in addition to tocopherols and sterols. Tocopherols and sterols have been industrially purified from SODD but no purification process for steryl esters has been developed. SODD was effic
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::a0d963440ccbca0ff47631b88a7ef40f
https://doi.org/10.1007/s11746-003-0700-6
https://doi.org/10.1007/s11746-003-0700-6
Publikováno v:
Journal of the American Oil Chemists' Society. 80:243-247
Conversion of oleic acid phytosteryl esters (OASE) to free phytosterols (referred to as sterols) by an enzymatic process was attempted. Enzymatic hydrolysis of OASE reached a steady state at 55–60% hydrolysis, but addition of methanol (MeOH) signif
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::d6caa69c0920b5c3905e3bad163dd51a
https://doi.org/10.1007/s11746-003-0684-2
https://doi.org/10.1007/s11746-003-0684-2
Autor:
Akio Sugihara, Yoshio Tominaga, Akiko Kawashima, Kengo Akimoto, Shigeaki Fujikawa, Yuji Shimada, Yomi Watanabe
Publikováno v:
Journal of the American Oil Chemists' Society. 80:37-42
A single-cell oil from a Mortierella alpina mutant (TGM17 oil) contains n−9 PUFA: 14.3 wt% 6,9-octadecadienoic acid (18∶2n−9; n−9 LnA) and 17.1 wt% Mead acid (20∶3n−9; MA). Lipase screening indicated that Pseudomonas aeruginosa lipase act
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::24ae6b935dac6fff0b75ba70d8b33313
https://doi.org/10.1007/s11746-003-0647-7
https://doi.org/10.1007/s11746-003-0647-7
Fractionation of Conjugated Linoleic Acid Isomers by Selective Hydrolysis with Candida rugosa Lipase
Autor:
Akio Sugihara, Toshihiro Nagao, Tadamasa Terai, Yuji Shimada, Yoshie Yamauchi-Sato, Takaya Yamamoto
Publikováno v:
Journal of Oleo Science. 52:367-374
It was attempted to prepare cis-9, trans-11 conjugated linoleic acid (c9,t11-CLA) and t10,c12-CLA concentrates that can be used as foods. A free fatty acid mixture (FFA-CLA) containing almost equal amounts of c9,t11- and t10,c12-CLAs was esterified w
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::8137ba1f0159fddb547f5756db3705e6
https://doi.org/10.5650/jos.52.367
https://doi.org/10.5650/jos.52.367