Zobrazeno 1 - 10
of 22
pro vyhledávání: '"Akinori Kashimura"'
Autor:
Eri Tabata, Ikuto Kobayashi, Takuya Morikawa, Akinori Kashimura, Peter O. Bauer, Fumitaka Oyama
Publikováno v:
iScience, Vol 26, Iss 8, Pp 107254- (2023)
Summary: Placental mammals' ancestors were insectivores, suggesting that modern mammals may have inherited the ability to digest insects. Acidic chitinase (Chia) is a crucial enzyme hydrolyzing significant component of insects' exoskeleton in many sp
Externí odkaz:
https://doaj.org/article/9fb2dd4b297a47de897af4261532ec99
Autor:
Eri Tabata, Akinori Kashimura, Satoshi Wakita, Misa Ohno, Masayoshi Sakaguchi, Yasusato Sugahara, Yoshihiro Kino, Vaclav Matoska, Peter O. Bauer, Fumitaka Oyama
Publikováno v:
Scientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
Abstract Chitin, a polymer of N-acetyl-D-glucosamine (GlcNAc), functions as a major structural component in crustaceans, insects and fungi and is the second most abundant polysaccharide in the nature. Although these chitin-containing organisms have b
Externí odkaz:
https://doaj.org/article/9c19af425e384cc88b5506bca30a9310
Autor:
Akinori Kashimura, Masahiro Kimura, Kazuaki Okawa, Hirotaka Suzuki, Atsushi Ukita, Satoshi Wakita, Kana Okazaki, Misa Ohno, Peter O. Bauer, Masayoshi Sakaguchi, Yasusato Sugahara, Fumitaka Oyama
Publikováno v:
International Journal of Molecular Sciences, Vol 16, Iss 2, Pp 4028-4042 (2015)
Mouse acidic mammalian chitinase (AMCase) plays important physiological roles in defense and nutrition. AMCase is composed of an N-terminal catalytic domain (CatD) and a C-terminal chitin-binding domain (CBD). We expressed CatD of mouse AMCase as a r
Externí odkaz:
https://doaj.org/article/b4709be3da2a4666abbad342eb06c303
Autor:
Masahiro Kimura, Satoshi Wakita, Kotarou Ishikawa, Kazutaka Sekine, Satoshi Yoshikawa, Akira Sato, Kazuaki Okawa, Akinori Kashimura, Masayoshi Sakaguchi, Yasusato Sugahara, Daisuke Yamanaka, Naohito Ohno, Peter O Bauer, Fumitaka Oyama
Publikováno v:
PLoS ONE, Vol 11, Iss 10, p e0164367 (2016)
Chitotriosidase (Chit1) is an enzyme associated with various diseases, including Gaucher disease, chronic obstructive pulmonary disease, Alzheimer disease and cystic fibrosis. In this study, we first expressed mouse mature Chit1 fused with V5 and (Hi
Externí odkaz:
https://doaj.org/article/50a4482394a14c24bd9636a01d64d139
Autor:
Eri Tabata, Akinori Kashimura, Satoshi Wakita, Masayoshi Sakaguchi, Yasusato Sugahara, Yasutada Imamura, Hideaki Shimizu, Vaclav Matoska, Peter O. Bauer, Fumitaka Oyama
Publikováno v:
International Journal of Molecular Sciences, Vol 19, Iss 2, p 362 (2018)
Acidic chitinase (Chia) has been implicated in asthma, allergic inflammations, and food processing. We have purified Chia enzymes with striking acid stability and protease resistance from chicken and pig stomach tissues using a chitin column and 8 M
Externí odkaz:
https://doaj.org/article/a9196d2cf8e245879a4fcfe03430a835
Autor:
Akinori Kashimura, Kazuaki Okawa, Kotarou Ishikawa, Yuta Kida, Kokoro Iwabuchi, Yudai Matsushima, Masayoshi Sakaguchi, Yasusato Sugahara, Fumitaka Oyama
Publikováno v:
PLoS ONE, Vol 8, Iss 11, p e78669 (2013)
Acidic mammalian chitinase (AMCase) has been shown to be associated with asthma in mouse models, allergic inflammation and food processing. Here, we describe an E. coli-expression system that allows for the periplasmic production of active AMCase fus
Externí odkaz:
https://doaj.org/article/d76125563a9b449f9617b0681749b15e
Autor:
Eri Tabata, Akihiro Itoigawa, Takumi Koinuma, Hiroshi Tayama, Akinori Kashimura, Masayoshi Sakaguchi, Vaclav Matoska, Peter O Bauer, Fumitaka Oyama
Publikováno v:
Molecular Biology and Evolution
Acidic chitinase (Chia) digests the chitin of insects in the omnivorous stomach and the chitinase activity in carnivorous Chia is significantly lower than that of the omnivorous enzyme. However, mechanistic and evolutionary insights into the function
Autor:
Erika Sasaki, Terumi Yurimoto, Masayoshi Sakaguchi, Akinori Kashimura, Satoshi Wakita, Fumitaka Oyama, Eri Tabata, Vaclav Matoska, Peter O. Bauer, Yasusato Sugahara, Maiko Uehara
Publikováno v:
Scientific Reports, Vol 9, Iss 1, Pp 1-11 (2019)
Scientific Reports
Scientific Reports
Chitin is a polymer of N-acetyl-D-glucosamine (GlcNAc) and a main constituent of insects’ exoskeleton. Insects are rich in protein with high energy conversion efficiency. Recently, we have reported that acidic chitinases (Chia) act as digestive enz
Autor:
Akinori Kashimura, Yasusato Sugahara, Fumitaka Oyama, Peter Bauer, Vaclav Matoska, Eri Tabata, Satoshi Wakita
Publikováno v:
Scientific Reports, Vol 9, Iss 1, Pp 1-11 (2019)
Scientific Reports
Scientific Reports
Commercially available porcine pepsin preparations have been used for the production of chitooligosaccharides with various biomedical activities. However, the origin of this activity is not well understood. Here we show that the chitosan-degrading ac
Autor:
Shunsuke Kobayashi, Fumitaka Oyama, Vaclav Matoska, Shotaro Honda, Yasusato Sugahara, Akinori Kashimura, Peter O. Bauer, Minori Kamaya, Masayoshi Sakaguchi, Masahiro Kimura, Satoshi Wakita
Publikováno v:
FEBS Letters. 591:3310-3318
Mouse acidic mammalian chitinase (AMCase) degrades chitin with highest efficiency at pH 2.0 and is active up to pH 8.0. Here, we report that mouse AMCase also exhibits transglycosylation activity under neutral conditions. We incubated natural and art