Zobrazeno 1 - 6
of 6
pro vyhledávání: '"Akiho Yoshida"'
A highly sensitive NanoLuc-based protease biosensor for detecting apoptosis and SARS-CoV-2 infection
Publikováno v:
Scientific Reports, Vol 13, Iss 1, Pp 1-11 (2023)
Abstract Proteases play critical roles in various biological processes, including apoptosis and viral infection. Several protease biosensors have been developed; however, obtaining a reliable signal from a very low level of endogenous protease activi
Externí odkaz:
https://doaj.org/article/9600a9a4fb65416e9fc5e6378d223f3d
Autor:
Izumi Oikawa, Shu Kondo, Kao Hashimoto, Akiho Yoshida, Megumi Hamajima, Hiromu Tanimoto, Katsuo Furukubo-Tokunaga, Ken Honjo
Publikováno v:
eLife, Vol 12 (2023)
Nociception is a neural process that animals have developed to avoid potentially tissue-damaging stimuli. While nociception is triggered in the peripheral nervous system, its modulation by the central nervous system is a critical process in mammals,
Externí odkaz:
https://doaj.org/article/f5310661bcb246f8a7356547f5eb2e95
Publikováno v:
STAR Protocols, Vol 4, Iss 2, Pp 102352- (2023)
Summary: An inability to proliferate at high temperatures typically gives viruses an attenuated phenotype. Here, we present a protocol to obtain and isolate temperature-sensitive (TS) SARS-CoV-2 strains via 5-fluorouracile-induced mutagenesis. We des
Externí odkaz:
https://doaj.org/article/e5a3684d04c0420498308e4bf1de0d22
Autor:
Akiho Yoshida, Shinya Okamura, Shiho Torii, Sayuri Komatsu, Paola Miyazato, Hitomi Sasaki, Shiori Ueno, Hidehiko Suzuki, Wataru Kamitani, Chikako Ono, Yoshiharu Matsuura, Shiro Takekawa, Koichi Yamanishi, Hirotaka Ebina
Publikováno v:
iScience, Vol 25, Iss 11, Pp 105412- (2022)
Summary: Live-attenuated vaccines are generally highly effective. Here, we aimed to develop one against SARS-CoV-2, based on the identification of three types of temperature-sensitive (TS) strains with mutations in nonstructural proteins (nsp), impai
Externí odkaz:
https://doaj.org/article/9c93efb921c54f8ab1c3c64c204d905b
Autor:
Izumi Oikawa, Shu Kondo, Kao Hashimoto, Akiho Yoshida, Megumi Hamajima, Hiromu Tanimoto, Katsuo Furukubo-Tokunaga, Ken Honjo
Nociception is a neural process that animals have developed to avoid potentially tissue-damaging stimuli. While nociception is triggered in the peripheral nervous system, its modulation by the central nervous system is a critical process in mammals,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::fdfd5d08ddeabe1e532bb37bbf9232e8
https://doi.org/10.1101/2022.03.08.483420
https://doi.org/10.1101/2022.03.08.483420
Autor:
Akiho Yoshida, Shinya Okamura, Shiho Torii, Sayuri Komatsu, Paola Miyazato, Shiori Ueno, Hidehiko Suzuki, Wataru Kamitani, Chikako Ono, Yoshiharu Matsuura, Shiro Takekawa, Koichi Yamanishi, Hirotaka Ebina
SUMMARYLive attenuated vaccines are generally highly effective. Here, we aimed to develop one against SARS-CoV-2, based on the identification of three types of temperature-sensitive (TS) strains with mutations in nonstructural proteins (nsp), impaire
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b31deaa59caa0199ee5a94a5631c0ee6
https://doi.org/10.1101/2021.02.15.430863
https://doi.org/10.1101/2021.02.15.430863