Zobrazeno 1 - 6
of 6
pro vyhledávání: '"Akihiro Kagita"'
Publikováno v:
Scientific Reports, Vol 13, Iss 1, Pp 1-17 (2023)
Abstract Myotonic dystrophy type 1 (DM1) is caused by expanded CTG repeats (CTGexp) in the dystrophia myotonica protein kinase (DMPK) gene, and the transcription products, expanded CUG repeats, sequester muscleblind like splicing regulator 1 (MBNL1),
Externí odkaz:
https://doaj.org/article/fa71afad8748492e95365ac375fec8b3
Autor:
Peter Gee, Mandy S. Y. Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F. Yang, Mio Iwasaki, Xiou H. Wang, Matthew A. Waller, Nanako Shirai, Yasuko O. Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita, Kumiko A. Iwabuchi, Masahiko Yasuda, Huaigeng Xu, Takeshi Noda, Jun Komano, Hidetoshi Sakurai, Naoto Inukai, Akitsu Hotta
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-18 (2020)
Expression of Cas9 and gRNA from viral vectors in vivo may cause off-target activity. Here the authors present NanoMEDIC, which uses nanovesicles to transiently deliver editing machinery to hard-to-transfect cells.
Externí odkaz:
https://doaj.org/article/4d0fe2ef9a604f0fb6865d1e08b1ed50
Autor:
Mitsuru Sasaki-Honda, Akihiro Kagita, Tatsuya Jonouchi, Toshiyuki Araki, Akitsu Hotta, Hidetoshi Sakurai
Publikováno v:
Stem Cell Research, Vol 47, Iss , Pp 101884- (2020)
Facioscapulohumeral muscular dystrophy type2 (FSHD2), which constitutes approximately 5% of total FSHD cases and develops the same symptoms as FSHD type 1 (FSHD1), is caused by various mutations in genes including SMCHD1. We report the generation and
Externí odkaz:
https://doaj.org/article/c3be678206374da98e378aa806f0028b
Autor:
Akitsu Hotta, Miyuki Ono, Peter Gee, Noriko Sasakawa, Takahiro Iguchi, Mandy S. Y. Lung, Xiou H. Wang, Akihiro Kagita, Huaigeng Xu, Yuto Kita
Publikováno v:
Stem Cell Reports
Summary Combined with CRISPR-Cas9 technology and single-stranded oligodeoxynucleotides (ssODNs), specific single-nucleotide alterations can be introduced into a targeted genomic locus in induced pluripotent stem cells (iPSCs); however, ssODN knockin
Autor:
Kumiko Iwabuchi, Akihiro Kagita, Lucy F. Yang, Nanako Shirai, Takahiro Iguchi, Yoko Fujita, Jun Komano, Takeshi Noda, Akitsu Hotta, Noriko Sasakawa, Yukimasa Makita, Matthew A. Waller, Naoto Inukai, Peter Gee, Mio Iwasaki, Mandy S. Y. Lung, Hidetoshi Sakurai, Yasuko O. Abe, Xiou H. Wang, Yasutomo Miura, Kei Watanabe, Masahiko Yasuda, Hiroyuki Hozumi, Huaigeng Xu, Yuya Okuzaki
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-18 (2020)
Nature Communications
Nature Communications
Prolonged expression of the CRISPR-Cas9 nuclease and gRNA from viral vectors may cause off-target mutagenesis and immunogenicity. Thus, a transient delivery system is needed for therapeutic genome editing applications. Here, we develop an extracellul
Autor:
Kaho Fujii, Fumiyo Kitaoka, Peter Gee, Akihiro Kagita, Shin Kaneko, Misato Nishikawa, Tomoko Takahashi, Akitsu Hotta, Huaigeng Xu, Bo Wang, Keisuke Okita, Yoshinori Yoshida, Masaki Nomura, Tatsuki Ueda, Noriko Sasakawa, Miyuki Ono
Publikováno v:
Cell Stem Cell. 24:566-578.e7
Summary Induced pluripotent stem cells (iPSCs) have strong potential in regenerative medicine applications; however, immune rejection caused by HLA mismatching is a concern. B2M gene knockout and HLA-homozygous iPSC stocks can address this issue, but