In vivo PAR-CLIP (viP-CLIP) of liver TIAL1 unveils targets regulating cholesterol synthesis and secretion

Autor: Hasan Vatandaslar, Aitor Garzia, Cindy Meyer, Svenja Godbersen, Laura T. L. Brandt, Esther Griesbach, Jeffrey A. Chao, Thomas Tuschl, Markus Stoffel

Publikováno v: Nature Communications, Vol 14, Iss 1, Pp 1-17 (2023)

Abstract System-wide cross-linking and immunoprecipitation (CLIP) approaches have unveiled regulatory mechanisms of RNA-binding proteins (RBPs) mainly in cultured cells due to limitations in the cross-linking efficiency of tissues. Here, we describe

Externí odkaz: https://doaj.org/article/e26c38132e814ef1ac94b1becc3a7648

Combination of antiviral drugs inhibits SARS-CoV-2 polymerase and exonuclease and demonstrates COVID-19 therapeutic potential in viral cell culture

Autor: Xuanting Wang, Carolina Q. Sacramento, Steffen Jockusch, Otávio Augusto Chaves, Chuanjuan Tao, Natalia Fintelman-Rodrigues, Minchen Chien, Jairo R. Temerozo, Xiaoxu Li, Shiv Kumar, Wei Xie, Dinshaw J. Patel, Cindy Meyer, Aitor Garzia, Thomas Tuschl, Patrícia T. Bozza, James J. Russo, Thiago Moreno L. Souza, Jingyue Ju

Publikováno v: Communications Biology, Vol 5, Iss 1, Pp 1-14 (2022)

In this paper, the hepatitis C virus inhibitors Pibrentasvir and Ombitasvir are found to inhibit the SARS-CoV-2 exonuclease and are shown to have therapeutic potential when combined with SARS-CoV-2 polymerase inhibitors in viral cell cultures.

Externí odkaz: https://doaj.org/article/421885d8a61b464da42eeec94cc924d8

AMPK mediates regulation of glomerular volume and podocyte survival

Autor: Khadija Banu, Qisheng Lin, John M. Basgen, Marina Planoutene, Chengguo Wei, Anand C. Reghuvaran, Xuefei Tian, Hongmei Shi, Felipe Garzon, Aitor Garzia, Nicholas Chun, Arun Cumpelik, Andrew D. Santeusanio, Weijia Zhang, Bhaskar Das, Fadi Salem, Li Li, Shuta Ishibe, Lloyd G. Cantley, Lewis Kaufman, Kevin V. Lemley, Zhaohui Ni, John Cijiang He, Barbara Murphy, Madhav C. Menon

Publikováno v: JCI Insight, Vol 6, Iss 19 (2021)

Herein, we report that Shroom3 knockdown, via Fyn inhibition, induced albuminuria with foot process effacement (FPE) without focal segmental glomerulosclerosis (FSGS) or podocytopenia. Interestingly, knockdown mice had reduced podocyte volumes. Human

Externí odkaz: https://doaj.org/article/48caabe5bbcf4213b0f25719c459b46d

The E3 ubiquitin ligase and RNA-binding protein ZNF598 orchestrates ribosome quality control of premature polyadenylated mRNAs

Autor: Aitor Garzia, Seyed Mehdi Jafarnejad, Cindy Meyer, Clément Chapat, Tasos Gogakos, Pavel Morozov, Mehdi Amiri, Maayan Shapiro, Henrik Molina, Thomas Tuschl, Nahum Sonenberg

Publikováno v: Nature Communications, Vol 8, Iss 1, Pp 1-10 (2017)

Translation of aberrant mRNAs causes ribosome stalling and translation arrest, followed by recycling of the stalled ribosome complex. Here the authors show that the Zinc Finger Protein 598 (ZNF598/Hel2) is implicated in sensing faulty translation of

Externí odkaz: https://doaj.org/article/f79858e0db6542bdb89c353b12ea9953

SARS-CoV-2 impairs interferon production via NSP2-induced repression of mRNA translation

Autor: Zhang Xu, Jung-Hyun Choi, David L. Dai, Jun Luo, Reese Jalal Ladak, Qian Li, Yimeng Wang, Christine Zhang, Shane Wiebe, Alex C. H. Liu, Xiaozhuo Ran, Jiaqi Yang, Parisa Naeli, Aitor Garzia, Lele Zhou, Niaz Mahmood, Qiyun Deng, Mohamed Elaish, Rongtuan Lin, Lara K. Mahal, Tom C. Hobman, Jerry Pelletier, Tommy Alain, Silvia M. Vidal, Thomas Duchaine, Mohammad T. Mazhab-Jafari, Xiaojuan Mao, Seyed Mehdi Jafarnejad, Nahum Sonenberg

Publikováno v: Xu, Z, Choi, J-H, Dai, D L, Luo, J, Ladak, R J, Li, Q, Wang, Y, Zhang, C, Wiebe, S, Liu, A C H, Ran, X, Yang, J, Naeli, P, Garzia, A, Zhou, L, Mahmood, N, Deng, Q, Elaish, M, Lin, R, Mahal, L K, Hobman, T C, Pelletier, J, Alain, T, Vidal, S M, Duchaine, T, Mazhab-Jafari, M T, Mao, X, Jafarnejad, S M & Sonenberg, N 2022, ' SARS-CoV-2 impairs interferon production via NSP2-induced repression of mRNA translation ', Proceedings of the National Academy of Sciences of the United States of America, vol. 119, no. 32, e2204539119 . https://doi.org/10.1073/pnas.2204539119

Viruses evade the innate immune response by suppressing the production or activity of cytokines such as type I interferons (IFNs). Here we report the discovery of a mechanism by which the SARS-CoV-2 virus coopts an intrinsic cellular machinery to sup

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6552dcb563bf1e40cb6a0eb4921c1a7c
https://pubmed.ncbi.nlm.nih.gov/35878012