Structure and rational engineering of the PglX methyltransferase and specificity factor for BREX phage defence

Autor: Sam C. Went, David M. Picton, Richard D. Morgan, Andrew Nelson, Aisling Brady, Giuseppina Mariano, David T. F. Dryden, Darren L. Smith, Nicolas Wenner, Jay C. D. Hinton, Tim R. Blower

Publikováno v: Nature Communications, Vol 15, Iss 1, Pp 1-18 (2024)

Abstract Bacteria have evolved a broad range of systems that provide defence against their viral predators, bacteriophages. Bacteriophage Exclusion (BREX) systems recognise and methylate 6 bp non-palindromic motifs within the host genome, and prevent

Externí odkaz: https://doaj.org/article/f9d96556945641aca3006a94eb0b5cec

Phage-inducible chromosomal islands promote genetic variability by blocking phage reproduction and protecting transductants from phage lysis.

Autor: Rodrigo Ibarra-Chávez, Aisling Brady, John Chen, José R Penadés, Andreas F Haag

Publikováno v: PLoS Genetics, Vol 18, Iss 3, p e1010146 (2022)

Phage-inducible chromosomal islands (PICIs) are a widespread family of highly mobile genetic elements that disseminate virulence and toxin genes among bacterial populations. Since their life cycle involves induction by helper phages, they are importa

Externí odkaz: https://doaj.org/article/5bf4e8ab572c4483bc1f7faa5c9165e9

Molecular Basis of Lysis–Lysogeny Decisions in Gram-Positive Phages

Autor: José R. Penadés, Alberto Marina, Nuria Quiles-Puchalt, Francisca Gallego del Sol, Aisling Brady, Alonso Felipe-Ruiz

Publikováno v: Annual Review Microbiology

versión pre-print: 30 páginas, 5 figuras
Temperate bacteriophages (phages) are viruses of bacteria. Upon infection of a susceptible host, a temperate phage can establish either a lytic cycle that kills the host or a lysogenic cycle as a stable

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7511e51f536ac6d5bf93848e67ad70b5
https://doi.org/10.1146/annurev-micro-033121-020757

Phage-inducible chromosomal islands promote genetic variability by blocking phage reproduction and protecting transductants from phage lysis

Autor: José R Penadés, John Chen, Victor Rodrigo Ibarra Chavez, Aisling Brady, Andreas Haag

Publikováno v: Ibarra-Chávez, R, Brady, A, Chen, J, Penadés, J R & Haag, A F 2022, ' Phage-inducible chromosomal islands promote genetic variability by blocking phage reproduction and protecting transductants from phage lysis ', PLOS Genetics, vol. 18, no. 3, e1010146 . https://doi.org/10.1371/journal.pgen.1010146

Funding: This work was supported by the following grants awarded to JPR: MR/M003876/1, MR/S00940X/1 and MR/V000772/1 from the Medical Research Council (MRC, UK; https://mrc.ukri.org); BB/N002873/1, BB/S003835/1 and BB/V002376/1 from the Biotechnology

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7c604302f774c300cf5ff1ffc8a19903
https://curis.ku.dk/ws/files/304781829/journal.pgen.1010146.pdf