Zobrazeno 1 - 9
of 9
pro vyhledávání: '"Aisha Yesbolatova"'
Autor:
Aisha Yesbolatova, Yuichiro Saito, Naomi Kitamoto, Hatsune Makino-Itou, Rieko Ajima, Risako Nakano, Hirofumi Nakaoka, Kosuke Fukui, Kanae Gamo, Yusuke Tominari, Haruki Takeuchi, Yumiko Saga, Ken-ichiro Hayashi, Masato T. Kanemaki
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-13 (2020)
Auxin-inducible degron systems can be leaky and require high doses of auxin. Here the authors establish AID2 which uses an OsTIR1 mutant and the ligand 5-Ph-IAA to overcome these problems and establish AID-mediated target depletion in mice.
Externí odkaz:
https://doaj.org/article/e96ad288aa7749f7bdb3f1e9cc207dea
Publikováno v:
Pharmaceuticals, Vol 13, Iss 5, p 103 (2020)
Conditional degron-based methods are powerful for studying protein function because a degron-fused protein can be rapidly and efficiently depleted by adding a defined ligand. Auxin-inducible degron (AID) is a popular technology by which a degron-fuse
Externí odkaz:
https://doaj.org/article/65718b3a72cb434d9ff7e7aa6a41d21a
Autor:
Risako Nakano, Aisha Yesbolatova, Yumiko Saga, Kosuke Fukui, Yuichiro Saito, Ken-ichiro Hayashi, Hirofumi Nakaoka, Kanae Gamo, Rieko Ajima, Naomi Kitamoto, Yusuke Tominari, Hatsune Makino-Itou, Masato T. Kanemaki, Haruki Takeuchi
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-13 (2020)
Nature Communications
Nature Communications
Protein knockdown using the auxin-inducible degron (AID) technology is useful to study protein function in living cells because it induces rapid depletion, which makes it possible to observe an immediate phenotype. However, the current AID system has
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::09efdb250e08d6e722861b8a11682068
http://link.springer.com/article/10.1038/s41467-020-19532-z
http://link.springer.com/article/10.1038/s41467-020-19532-z
Publikováno v:
Methods. :73-80
Controlling protein expression using a degron is advantageous because the protein of interest can be rapidly depleted in a reversible manner. We pioneered the development of the auxin-inducible degron (AID) technology by transplanting a plant-specifi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aecce9ace8ce75d9ebd607bd18e82113
https://doi.org/10.1016/j.ymeth.2019.04.010
https://doi.org/10.1016/j.ymeth.2019.04.010
Publikováno v:
Pharmaceuticals
Volume 13
Issue 5
Pharmaceuticals, Vol 13, Iss 103, p 103 (2020)
Volume 13
Issue 5
Pharmaceuticals, Vol 13, Iss 103, p 103 (2020)
Conditional degron-based methods are powerful for studying protein function because a degron-fused protein can be rapidly and efficiently depleted by adding a defined ligand. Auxin-inducible degron (AID) is a popular technology by which a degron-fuse
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f7ac3cf39bfe1f04683a1ae76a3bb151
Controlling protein expression using a degron is drawing more attention because the protein of interest can be rapidly depleted in a reversible manner. We pioneered the development of the auxin-inducible degron (AID) technology by transplanting a pla
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ebaa10ac06ad71d4b809b5b0bf4725e0
Publikováno v:
Drug discovery today. Technologies. 31
Targeted protein degraders, known as proteolysis targeting chimeras (PROTACs), are drawing more attention as next-generation drugs to target currently undruggable proteins. As drug discovery of functional degraders involves time- and cost-consuming l
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cdac0760851ed914a3aa073fdd2c795e
https://pubmed.ncbi.nlm.nih.gov/31200864
https://pubmed.ncbi.nlm.nih.gov/31200864
Autor:
Masato T. Kanemaki, Aisha Yesbolatova
Publikováno v:
Nature Chemical Biology. 14:414-415
Proteins brought to an E3 ubiquitin ligase are polyubiquitylated for rapid degradation via the proteasome. A new study developed a tag-based degradation system using heterobifunctional degradation inducers called dTAGs, which rapidly depleted FKBP12F
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::85b7af33ead5bb03000943add537690b
https://doi.org/10.1038/s41589-018-0024-5
https://doi.org/10.1038/s41589-018-0024-5