Application of CRISPR-Cas12a temperature sensitivity for improved genome editing in rice, maize, and Arabidopsis

Autor: Aimee A. Malzahn, Xu Tang, Keunsub Lee, Qiurong Ren, Simon Sretenovic, Yingxiao Zhang, Hongqiao Chen, Minjeong Kang, Yu Bao, Xuelian Zheng, Kejun Deng, Tao Zhang, Valeria Salcedo, Kan Wang, Yong Zhang, Yiping Qi

Publikováno v: BMC Biology, Vol 17, Iss 1, Pp 1-14 (2019)

Abstract Background CRISPR-Cas12a (formerly Cpf1) is an RNA-guided endonuclease with distinct features that have expanded genome editing capabilities. Cas12a-mediated genome editing is temperature sensitive in plants, but a lack of a comprehensive un

Externí odkaz: https://doaj.org/article/90a2e580ce324569b3ff72d77e2eb6cb

Boosting plant genome editing with a versatile CRISPR-Combo system

Autor: Changtian Pan, Gen Li, Aimee A. Malzahn, Yanhao Cheng, Benjamin Leyson, Simon Sretenovic, Filiz Gurel, Gary D. Coleman, Yiping Qi

Publikováno v: Nature plants. 8(5)

CRISPR-Cas9, its derived base editors and CRISPR activation systems have greatly aided genome engineering in plants. However, these systems are mostly used separately, leaving their combinational potential largely untapped. Here we develop a versatil

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f93f900783654f5727f55bd87c59ba9b
https://pubmed.ncbi.nlm.nih.gov/35596077

CRISPR-Act3.0 for highly efficient multiplexed gene activation in plants

Autor: Changtian, Pan, Xincheng, Wu, Kasey, Markel, Aimee A, Malzahn, Neil, Kundagrami, Simon, Sretenovic, Yingxiao, Zhang, Yanhao, Cheng, Patrick M, Shih, Yiping, Qi

Publikováno v: Nature plants. 7(7)

RNA-guided CRISPR activation (CRISPRa) systems have been developed in plants. However, the simultaneous activation of multiple genes remains challenging. Here, we develop a highly robust CRISPRa system working in rice, Arabidopsis and tomato, CRISPR-

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::517215448d2028517b08d4d8ec7a3a64
https://pubmed.ncbi.nlm.nih.gov/34168320

Activities and specificities of CRISPR/Cas9 and Cas12a nucleases for targeted mutagenesis in maize.

Autor: Lee K; Crop Bioengineering Center, Iowa State University, Ames, IA, USA.; Department of Agronomy, Iowa State University, Ames, IA, USA., Zhang Y; Department of Plant Science and Landscape Architecture, University of Maryland, College Park, MD, USA., Kleinstiver BP; Molecular Pathology Unit, Center for Cancer Research, and Center for Computational and Integrative Biology, Massachusetts General Hospital, Charlestown, MA, USA.; Department of Pathology, Harvard Medical School, Boston, MA, USA., Guo JA; Molecular Pathology Unit, Center for Cancer Research, and Center for Computational and Integrative Biology, Massachusetts General Hospital, Charlestown, MA, USA., Aryee MJ; Department of Pathology, Harvard Medical School, Boston, MA, USA.; Department of Biostatistics, Harvard T.H. Chan School of Public Health, Boston, MA, USA., Miller J; Crop Bioengineering Center, Iowa State University, Ames, IA, USA., Malzahn A; Department of Plant Science and Landscape Architecture, University of Maryland, College Park, MD, USA., Zarecor S; Crop Bioengineering Center, Iowa State University, Ames, IA, USA.; Department of Genetics, Development and Cell Biology, Iowa State University, Ames, IA, USA., Lawrence-Dill CJ; Crop Bioengineering Center, Iowa State University, Ames, IA, USA.; Department of Agronomy, Iowa State University, Ames, IA, USA.; Department of Genetics, Development and Cell Biology, Iowa State University, Ames, IA, USA.; Bioinformatics and Computational Biology Program, Iowa State University, Ames, IA, USA., Joung JK; Molecular Pathology Unit, Center for Cancer Research, and Center for Computational and Integrative Biology, Massachusetts General Hospital, Charlestown, MA, USA.; Department of Pathology, Harvard Medical School, Boston, MA, USA., Qi Y; Department of Plant Science and Landscape Architecture, University of Maryland, College Park, MD, USA.; Institute for Bioscience and Biotechnology Research, University of Maryland, Rockville, MD, USA., Wang K; Crop Bioengineering Center, Iowa State University, Ames, IA, USA.; Department of Agronomy, Iowa State University, Ames, IA, USA.

Publikováno v: Plant biotechnology journal [Plant Biotechnol J] 2019 Feb; Vol. 17 (2), pp. 362-372. Date of Electronic Publication: 2018 Jul 22.

A large-scale whole-genome sequencing analysis reveals highly specific genome editing by both Cas9 and Cpf1 (Cas12a) nucleases in rice.

Autor: Tang X; Department of Biotechnology, School of Life Sciences and Technology, Center for Informational Biology, University of Electronic Science and Technology of China, Room 216, Main Building, No. 4, Section 2, North Jianshe Road, Chengdu, 610054, People's Republic of China., Liu G; Jiangsu Key Laboratory of Crop Genetics and Physiology, Co-Innovation Center for Modern Production Technology of Grain Crops, Key Laboratory of Plant Functional Genomics of the Ministry of Education, Yangzhou University, Yangzhou, 225009, China.; Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou, 225009, China., Zhou J; Department of Biotechnology, School of Life Sciences and Technology, Center for Informational Biology, University of Electronic Science and Technology of China, Room 216, Main Building, No. 4, Section 2, North Jianshe Road, Chengdu, 610054, People's Republic of China., Ren Q; Department of Biotechnology, School of Life Sciences and Technology, Center for Informational Biology, University of Electronic Science and Technology of China, Room 216, Main Building, No. 4, Section 2, North Jianshe Road, Chengdu, 610054, People's Republic of China., You Q; Jiangsu Key Laboratory of Crop Genetics and Physiology, Co-Innovation Center for Modern Production Technology of Grain Crops, Key Laboratory of Plant Functional Genomics of the Ministry of Education, Yangzhou University, Yangzhou, 225009, China.; Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou, 225009, China., Tian L; Department of Biotechnology, School of Life Sciences and Technology, Center for Informational Biology, University of Electronic Science and Technology of China, Room 216, Main Building, No. 4, Section 2, North Jianshe Road, Chengdu, 610054, People's Republic of China., Xin X; Department of Biotechnology, School of Life Sciences and Technology, Center for Informational Biology, University of Electronic Science and Technology of China, Room 216, Main Building, No. 4, Section 2, North Jianshe Road, Chengdu, 610054, People's Republic of China., Zhong Z; Department of Biotechnology, School of Life Sciences and Technology, Center for Informational Biology, University of Electronic Science and Technology of China, Room 216, Main Building, No. 4, Section 2, North Jianshe Road, Chengdu, 610054, People's Republic of China., Liu B; Department of Biotechnology, School of Life Sciences and Technology, Center for Informational Biology, University of Electronic Science and Technology of China, Room 216, Main Building, No. 4, Section 2, North Jianshe Road, Chengdu, 610054, People's Republic of China., Zheng X; Department of Biotechnology, School of Life Sciences and Technology, Center for Informational Biology, University of Electronic Science and Technology of China, Room 216, Main Building, No. 4, Section 2, North Jianshe Road, Chengdu, 610054, People's Republic of China., Zhang D; Department of Biotechnology, School of Life Sciences and Technology, Center for Informational Biology, University of Electronic Science and Technology of China, Room 216, Main Building, No. 4, Section 2, North Jianshe Road, Chengdu, 610054, People's Republic of China., Malzahn A; Department of Plant Science and Landscape Architecture, University of Maryland, College Park, MD, 20742, USA., Gong Z; Jiangsu Key Laboratory of Crop Genetics and Physiology, Co-Innovation Center for Modern Production Technology of Grain Crops, Key Laboratory of Plant Functional Genomics of the Ministry of Education, Yangzhou University, Yangzhou, 225009, China., Qi Y; Department of Plant Science and Landscape Architecture, University of Maryland, College Park, MD, 20742, USA. Yiping@umd.edu.; Institute for Bioscience and Biotechnology Research, University of Maryland, Rockville, MD, 20850, USA. Yiping@umd.edu., Zhang T; Jiangsu Key Laboratory of Crop Genetics and Physiology, Co-Innovation Center for Modern Production Technology of Grain Crops, Key Laboratory of Plant Functional Genomics of the Ministry of Education, Yangzhou University, Yangzhou, 225009, China. zhangtao@yzu.edu.cn.; Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou, 225009, China. zhangtao@yzu.edu.cn., Zhang Y; Department of Biotechnology, School of Life Sciences and Technology, Center for Informational Biology, University of Electronic Science and Technology of China, Room 216, Main Building, No. 4, Section 2, North Jianshe Road, Chengdu, 610054, People's Republic of China. zhangyong916@uestc.edu.cn.

Publikováno v: Genome biology [Genome Biol] 2018 Jul 04; Vol. 19 (1), pp. 84. Date of Electronic Publication: 2018 Jul 04.