Zobrazeno 1 - 9
of 9
pro vyhledávání: '"Aigar Kommer"'
Publikováno v:
Protein Science. 15:242-247
Molecular chaperones of the Hsp70 family (bacterial DnaK, DnaJ, and GrpE) were shown to be strictly required for refolding of firefly luciferase from a denatured state and thus for effective restoration of its activity. At the same time the luciferas
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8c0aa8af1eda54898ba03bbef1761406
https://doi.org/10.1110/ps.051752506
https://doi.org/10.1110/ps.051752506
Publikováno v:
Journal of Biological Chemistry. 272:10646-10651
Globin synthesis in a wheat germ cell-free translation system was performed in the presence of [3H]hemin and [35S]methionine to determine the minimal length of the nascent ribosome-bound globin chain capable of heme binding. Nascent polypeptides of p
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0395b45e1c13d8c94f99f35b72cf1698
https://doi.org/10.1074/jbc.272.16.10646
https://doi.org/10.1074/jbc.272.16.10646
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 375
Protein synthesis in cell-free systems is an emerging technology already competing with in vivo expression methods. In this chapter the basic principles of continuous-exchange protein synthesizing systems, and protocols for Escherichia coli and wheat
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::42cdcfa99cc068c0013f3197194994c8
https://pubmed.ncbi.nlm.nih.gov/17634595
https://pubmed.ncbi.nlm.nih.gov/17634595
Publikováno v:
In Vitro Transcription and Translation Protocols ISBN: 9781588295583
Protein synthesis in cell-free systems is an emerging technology already competing with in vivo expression methods. In this chapter the basic principles of continuous-exchange protein synthesizing systems, and protocols for Escherichia coli and wheat
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3b8a7b4cd19a95d725653bdc150eda8f
https://doi.org/10.1385/1-59745-388-9:19
https://doi.org/10.1385/1-59745-388-9:19
Publikováno v:
FEBS letters. 580(30)
A comparative study of the 30S ribosomal subunit in the complex with protein S1 and the subunit depleted of this protein has been carried out by the hot tritium bombardment method. Differences in exposure of some ribosomal proteins within the 30S sub
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d3ef5059c1813cf7d50b11b786f94a47
https://pubmed.ncbi.nlm.nih.gov/17150214
https://pubmed.ncbi.nlm.nih.gov/17150214
Autor:
O. N. Pavlova, Vyacheslav A. Kolb, Konstantin Severinov, Anastasia Metlitskaya, Igor A. Krasheninnikov, Teymur Kazakov, Michael Ibba, Mette Prætorius-Ibba, Inessa A. Khmel, Aigar Kommer
Publikováno v:
The Journal of biological chemistry. 281(26)
Microcin C is a ribosome-synthesized heptapeptide that contains a modified adenosine monophosphate covalently attached to the C-terminal aspartate. Microcin C is a potent inhibitor of bacterial cell growth. Based on the in vivo kinetics of inhibition
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9992b476a3f11c166bede6aa5cc0730a
https://pubmed.ncbi.nlm.nih.gov/16574659
https://pubmed.ncbi.nlm.nih.gov/16574659
Publikováno v:
Cell-Free Translation Systems ISBN: 9783642639562
Native structure formation of newly synthesized proteins is a key problem relating to the cell-free translation technology that is aimed at the synthesis of biologically active products. At the same time, a lot of experimental data indicate that the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::465a34d0400b9e5b5b34bd30b0bae1ff
https://doi.org/10.1007/978-3-642-59379-6_11
https://doi.org/10.1007/978-3-642-59379-6_11
Publikováno v:
Biochemistry and cell biology = Biochimie et biologie cellulaire. 73(11-12)
Many unfolded polypeptides are capable of refolding into their native structure upon the removal of the denaturant. However, the folding of the mature protein during renaturation does not accurately reflect the folding process of nascent proteins in
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::119dd4e31150244c3f918b8ade65bf9e
https://pubmed.ncbi.nlm.nih.gov/8722039
https://pubmed.ncbi.nlm.nih.gov/8722039
Publikováno v:
FEBS Letters. (1-3):261-263
Globin synthesis in cell-free extracts of rabbit reticulocytes was carried out in the presence of 3H-labeled hemin. Sucrose gradient centrifugation analysis revealed [3H]hemin in the polyribosome fraction. The addition of puromycin resulted in the re
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0359e1643225b74dfde43182b77f6737