Analysis of the ARTIC V4 and V4.1 SARS-CoV-2 primers and their impact on the detection of Omicron BA.1 and BA.2 lineage-defining mutations

Autor: Fatima R. Ulhuq, Madhuri Barge, Kerry Falconer, Jonathan Wild, Goncalo Fernandes, Abbie Gallagher, Suzie McGinley, Ahmad Sugadol, Muhammad Tariq, Daniel Maloney, Juliet Kenicer, Rebecca Dewar, Kate Templeton, Martin P. McHugh

Publikováno v: Ulhuq, F R, Barge, M, Falconer, K, Wild, J, Fernandes, G, Gallagher, A, Mcginley, S, Sugadol, A, Tariq, M, Maloney, D, Kenicer, J, Dewar, R, Templeton, K & Mchugh, M P 2023, ' Analysis of the ARTIC V4 and V4.1 SARS-CoV-2 primers and their impact on the detection of Omicron BA.1 and BA.2 lineage-defining mutations ', Microbial Genomics, vol. 9, no. 4, 000991 . https://doi.org/10.1099/mgen.0.000991

The ARTIC protocol uses a multiplexed PCR approach with two primer pools tiling the entire SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) genome. Primer pool updates are necessary for accurate amplicon sequencing of evolving SARS-CoV-2

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::90b9877ca84b5091540179760e29142d
https://doi.org/10.1099/mgen.0.000991