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Efficient Generation of Multi-gene Knockout Cell Lines and Patient-derived Xenografts Using Multi-colored Lenti-CRISPR-Cas9.

Autor: Behrmann L; Department of Oncology and Children's Research Center, University Children's Hospital Zürich, Zürich, Switzerland., McComb S; Department of Oncology and Children's Research Center, University Children's Hospital Zürich, Zürich, Switzerland.; Present address: Human Health and Therapeutics, National Research Council, Ottawa, Canada., Aguadé-Gorgorió J; Department of Oncology and Children's Research Center, University Children's Hospital Zürich, Zürich, Switzerland., Huang Y; Department of Oncology and Children's Research Center, University Children's Hospital Zürich, Zürich, Switzerland., Hermann M; Institute of Laboratory Animal Science, University of Zurich, Zürich, Switzerland., Pelczar P; Institute of Laboratory Animal Science, University of Zurich, Zürich, Switzerland.; Present address: Center for Transgenic Models, University of Basel, Basel, Switzerland., Aguzzi A; Institute of Neuropathology, University Hospital of Zurich, Zurich, Switzerland., Bourquin JP; Department of Oncology and Children's Research Center, University Children's Hospital Zürich, Zürich, Switzerland., Bornhauser BC; Department of Oncology and Children's Research Center, University Children's Hospital Zürich, Zürich, Switzerland.

Publikováno v: Bio-protocol [Bio Protoc] 2017 Apr 05; Vol. 7 (7), pp. e2222. Date of Electronic Publication: 2017 Apr 05 (Print Publication: 2017).