Zobrazeno 1 - 10
of 22
pro vyhledávání: '"Agnieszka M. Murakami"'
Autor:
Agnieszka M. Murakami, Katsuhiro Nagatomo, Ichro Miyoshi, Shirou Itagaki, Yasutaka Niwa, Manabu Murakami
Publikováno v:
Scientific Reports, Vol 13, Iss 1, Pp 1-11 (2023)
Abstract We developed a new method to analyze protein–protein interactions using a dual-inducible prokaryotic expression system. To evaluate protein–protein binding, a chimeric fusion toxin gene was constructed using a DNase-treated short DNA fra
Externí odkaz:
https://doaj.org/article/70a91ba58ced46d297cebbfc66c5fdb0
Autor:
Agnieszka M. Murakami, Manabu Yonekura, Katsuhiro Nagatomo, Yasutaka Niwa, Shirou Itagaki, Manabu Murakami
Publikováno v:
MethodsX, Vol 10, Iss , Pp 102167- (2023)
DNA recombination is a useful technology for cloning and subsequent functional analysis, while standard techniques for plasmid DNA recombination have remained unchanged. In the present study, we introduced rapid method for plasmid DNA recombination,
Externí odkaz:
https://doaj.org/article/6707168ea2e140b7b2b23135ac425f0c
Autor:
Manabu Murakami, Feng Xu, Takayoshi Ohba, Takeshi Kobayashi, Yoshiro Inoue, Agnieszka M. Murakami, Ichiro Miyoshi, Kyoichi Ono, Noritsugu Tohse
Publikováno v:
Journal of Pharmacological Sciences, Vol 145, Iss 3, Pp 253-261 (2021)
Facilitation of cardiac function in response to signals from the sympathetic nervous system is initiated by the phosphorylation of L-type voltage-dependent Ca2+ channels (VDCCs) by protein kinase A (PKA), which in turn is activated by β-adrenoceptor
Externí odkaz:
https://doaj.org/article/92d608b1bac14dea80ab12b7cd05b0cf
Publikováno v:
PLoS ONE, Vol 16, Iss 10 (2021)
In this study, we introduced an efficient subcloning and expression system with two inducible prokaryotic expression promoters, arabinose and lac, in a single plasmid in Escherichia coli. The arabinose promoter unit allows for the expression of a FLA
Externí odkaz:
https://doaj.org/article/c22896a72e1d4403b3c1b5d392e166a1
Autor:
Manabu Murakami, Agnieszka M Murakami, Manabu Yonekura, Ichiro Miyoshi, Shirou Itagaki, Yasutaka Niwa
Publikováno v:
PNAS Nexus. 2
We introduce a simple, dual direct cloning plasmid system (pgMAX-II) for gene expression analysis in both prokaryotic (Escherichia coli) and mammalian cells. This system, which uses a prokaryotic expression unit adapted from the pgMAX system and a ma
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::05523d9b02a3957b62333377ef8845d0
https://doi.org/10.1093/pnasnexus/pgad139
https://doi.org/10.1093/pnasnexus/pgad139
Autor:
Takeshi Kobayashi, Agnieszka M. Murakami, Kyoichi Ono, Yoshiro Inoue, Takayoshi Ohba, Noritsugu Tohse, Manabu Murakami, Feng Xu, Ichiro Miyoshi
Publikováno v:
Journal of Pharmacological Sciences, Vol 145, Iss 3, Pp 253-261 (2021)
Facilitation of cardiac function in response to signals from the sympathetic nervous system is initiated by the phosphorylation of L-type voltage-dependent Ca2+ channels (VDCCs) by protein kinase A (PKA), which in turn is activated by β-adrenoceptor
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0efa46ddd3cf8b0c6a46beda43b93588
https://doi.org/10.1016/j.jphs.2020.12.011
https://doi.org/10.1016/j.jphs.2020.12.011
Autor:
Manabu Murakami, Agnieszka M. Murakami, Takayuki Nemoto, Takayoshi Ohba, Manabu Yonekura, Yuichi Toyama, Hirofumi Tomita, Yasushi Matsuzaki, Daisuke Sawamura, Kazuyoshi Hirota, Shirou Itagaki, Yujiro Asada, Ichiro Miyoshi
Publikováno v:
PLoS ONE, Vol 17, Iss 1, p e0261668 (2022)
PLoS ONE
PLoS ONE
Polycystic kidney disease (PKD) is the most common genetic cause of kidney failure in humans. Among the various PKD-related molecules, PKD2L1 forms cation channels, but its physiological importance is obscure. In the present study, we established a t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::83b4c89407382594f539b37eff415658
https://doaj.org/article/2545abb773364570b4c6b9dd7db9c2de
https://doaj.org/article/2545abb773364570b4c6b9dd7db9c2de
In this study, we introduced an efficient subcloning and expression system with two inducible prokaryotic expression promoters, arabinose and lac, in a single plasmid in Escherichia coli. This pdMAX system is a manageable size at 5811 bp. The arabino
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::2b55b8ac2c2697e03234a39a4f3be9ee
https://doi.org/10.1101/2020.07.23.217315
https://doi.org/10.1101/2020.07.23.217315
We introduce an efficient subcloning and expression plasmid system with two different modes (prokaryotic for expression in Escherichia coli with lac promoter and mammalian modes with cytomegalovirus promoter). The efficient subcloning (DNA insertion)
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::6fcc8f8b6b96da97f634fe0947f833b1
https://doi.org/10.1101/2020.05.21.108126
https://doi.org/10.1101/2020.05.21.108126
Publikováno v:
PLoS ONE, Vol 16, Iss 10, p e0258553 (2021)
PLoS ONE
PLoS ONE, Vol 16, Iss 10 (2021)
PLoS ONE
PLoS ONE, Vol 16, Iss 10 (2021)
In this study, we introduced an efficient subcloning and expression system with two inducible prokaryotic expression promoters, arabinose and lac, in a single plasmid in Escherichia coli. The arabinose promoter unit allows for the expression of a FLA
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::15e88656f15af318406af5cc3fd81b7d
https://doi.org/10.1371/journal.pone.0258553
https://doi.org/10.1371/journal.pone.0258553