Zobrazeno 1 - 5
of 5
pro vyhledávání: '"Agnieszka Lass-Napiorkowska"'
Publikováno v:
eLife, Vol 4 (2015)
Initiation of transcription is a primary means for controlling gene expression. In bacteria, the RNA polymerase (RNAP) holoenzyme binds and unwinds promoter DNA, forming the transcription bubble of the open promoter complex (RPo). We have determined
Externí odkaz:
https://doaj.org/article/4268cf4aea50443fa856258e8b09015b
Autor:
Jesse Hauver, Robert Landick, Andrey Feklistov, Karl-Heinz Altmann, Brian Bae, Agnieszka Lass-Napiorkowska, Tomasz Heyduk, Markus Kalesse, Seth A. Darst, Florian Glaus
Trapping RNA polymerase in the act The enzyme RNA polymerase (RNAP) finds promoter elements in the genome, separates (or “melts”) the DNA strands, and transcribes the template DNA strand to give RNA. A mobile clamp in RNAP plays a key role in ini
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b3bfbf24a2beab4a068844a56151e3b3
https://europepmc.org/articles/PMC5696265/
https://europepmc.org/articles/PMC5696265/
RNA polymerase (RNAP) backtracking is a backward sliding of the enzyme along DNA and RNA. It plays important roles in many essential processes in bacteria and in eukaryotes. We describe here a fluorescence-based approach that allows a real-time obser
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3c53b7b83af3c88921300a7727655747
https://europepmc.org/articles/PMC5921838/
https://europepmc.org/articles/PMC5921838/
Publikováno v:
Analytical chemistry. 84(7)
We have recently developed a mix-and-read format homogeneous antigen peptide based assay for detection of the antibodies (Tian, L.; Heyduk, T. Anal. Chem. 2009, 81, 5218-5225) that employed for target detection a simple biophysical mechanism of targe