Zobrazeno 1 - 10
of 34
pro vyhledávání: '"Agnieszka Gambus"'
Publikováno v:
STAR Protocols, Vol 5, Iss 3, Pp 103237- (2024)
Summary: Here, we present a large-scale FLAG immunoprecipitation protocol to isolate large protein complexes driving DNA replication at replicating chromatin assembled in Xenopus laevis egg extract. We describe how to prepare demembranated sperm nucl
Externí odkaz:
https://doaj.org/article/d3a3b49bd9604fb0a3137a6c26adea9e
Autor:
Rebecca M. Jones, Joaquin Herrero Ruiz, Shaun Scaramuzza, Sarmi Nath, Chaoyu Liu, Marta Henklewska, Toyoaki Natsume, Robert G. Bristow, Francisco Romero, Masato T. Kanemaki, Agnieszka Gambus
Publikováno v:
iScience, Vol 27, Iss 7, Pp 110260- (2024)
Summary: To ensure timely duplication of the entire eukaryotic genome, thousands of replication machineries (replisomes) act on genomic DNA at any time during S phase. In the final stages of this process, replisomes are unloaded from chromatin. Unloa
Externí odkaz:
https://doaj.org/article/1301c90532be429a8d1333f2902214f5
Autor:
Camilla S. Colding-Christensen, Ellen S. Kakulidis, Javier Arroyo-Gomez, Ivo A. Hendriks, Connor Arkinson, Zita Fábián, Agnieszka Gambus, Niels Mailand, Julien P. Duxin, Michael L. Nielsen
Publikováno v:
Nature Communications, Vol 14, Iss 1, Pp 1-19 (2023)
Abstract Ubiquitin widely modifies proteins, thereby regulating most cellular functions. The complexity of ubiquitin signalling necessitates unbiased methods enabling global detection of dynamic protein ubiquitylation. Here, we describe UBIMAX (UBiqu
Externí odkaz:
https://doaj.org/article/c45c5dfb98ea4d949da491f277c33fef
Autor:
Shaun Scaramuzza, Rebecca M. Jones, Martina Muste Sadurni, Alicja Reynolds-Winczura, Divyasree Poovathumkadavil, Abigail Farrell, Toyoaki Natsume, Patricia Rojas, Cyntia Fernandez Cuesta, Masato T. Kanemaki, Marco Saponaro, Agnieszka Gambus
Publikováno v:
Nature Communications, Vol 14, Iss 1, Pp 1-20 (2023)
Abstract Cell division is the basis for the propagation of life and requires accurate duplication of all genetic information. DNA damage created during replication (replication stress) is a major cause of cancer, premature aging and a spectrum of oth
Externí odkaz:
https://doaj.org/article/1ddaed01eda54eabb32edba52fee2e2b
Publikováno v:
Biology, Vol 13, Iss 4, p 233 (2024)
The eukaryotic replicative helicase (CMG complex) is assembled during DNA replication initiation in a highly regulated manner, which is described in depth by other manuscripts in this Issue. During DNA replication, the replicative helicase moves thro
Externí odkaz:
https://doaj.org/article/3cd5476b76014f9289bf922473c3813b
Autor:
Yavor Hadzhiev, Haseeb K. Qureshi, Lucy Wheatley, Ledean Cooper, Aleksandra Jasiulewicz, Huy Van Nguyen, Joseph W. Wragg, Divyasree Poovathumkadavil, Sascha Conic, Sarah Bajan, Attila Sik, György Hutvàgner, Làszlò Tora, Agnieszka Gambus, John S. Fossey, Ferenc Müller
Publikováno v:
Nature Communications, Vol 10, Iss 1, Pp 1-14 (2019)
Transcription is globally repressed in early stage of embryo development, but a set of genes including pri-miR-430 and zinc finger genes is known to escape the repression. Here the authors image the very first transcriptional activities in the living
Externí odkaz:
https://doaj.org/article/c687a4f0c35544f7aae2b16326e305e8
Autor:
Sara Priego Moreno, Agnieszka Gambus
Publikováno v:
Genes, Vol 6, Iss 3, Pp 451-468 (2015)
Posttranslational modification of proteins by means of attachment of a small globular protein ubiquitin (i.e., ubiquitylation) represents one of the most abundant and versatile mechanisms of protein regulation employed by eukaryotic cells. Ubiquityla
Externí odkaz:
https://doaj.org/article/78972f73e42e427c94a1a62ea657b93d
Autor:
Camilla S. Colding-Christensen, Ellen S. Kakulidis, Javier Arroyo-Gomez, Ivo A. Hendriks, Connor Arkinson, Zita Fábián, Agnieszka Gambus, Niels Mailand, Julien P. Duxin, Michael L. Nielsen
Ubiquitin widely modifies proteins, thereby regulating most cellular functions. The complexity of ubiquitin signalling necessitates unbiased methods enabling global detection of dynamic protein ubiquitylation. Here, we describe UBIMAX (UBiquitin targ
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b13890b5da4774cae5b948c4358d1c02
https://doi.org/10.1101/2023.05.15.540799
https://doi.org/10.1101/2023.05.15.540799
Autor:
Rebecca M. Jones, Joaquin Herrero Ruiz, Shaun Scaramuzza, Sarmi Nath, Marta Henklewska, Toyoaki Natsume, Francisco Romero, Masato T. Kanemaki, Agnieszka Gambus
To ensure faultless duplication of the entire genome, eukaryotic replication initiates from thousands of replication origins. Replication forks emanating from origins move through the chromatin until they encounter forks from neighbouring origins, at
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::893a3589aad5aad8d1ad84656be02df0
https://doi.org/10.1101/2022.07.12.499744
https://doi.org/10.1101/2022.07.12.499744
Autor:
Shaun Scaramuzza, Martina Muste Sadurni, Divyasree Poovathumkadavil, Toyoaki Natsume, Patricia Rojas, Masato T. Kanemaki, Marco Saponaro, Agnieszka Gambus
Cell division is the basis for the propagation of life and requires accurate duplication of all genetic information. DNA damage created during replication (replication stress) is a major cause of cancer, premature aging and a spectrum of other human
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::321e184875841c48508c45d1abe7c6ff
https://doi.org/10.1101/2022.03.23.485338
https://doi.org/10.1101/2022.03.23.485338