Zobrazeno 1 - 4
of 4
pro vyhledávání: '"Agnes M. Kanikula"'
Publikováno v:
Protein Expression and Purification. 18:20-26
alpha-Glucosidases (EC 3.2.1.20) are recognized as important in starch degradation during cereal seed germination. A barley (Hordeum vulgare) alpha-glucosidase expressed in Pichia pastoris was cultured in flasks; however, the yield was low necessitat
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::490a89b044c8a34414ac854101e08870
https://doi.org/10.1006/prep.1999.1160
https://doi.org/10.1006/prep.1999.1160
Autor:
Hazel M. Holden, Matthew M. Benning, Joshua Sakon, Ivan Rayment, Agnes M. Kanikula, Hans H. Liao
Publikováno v:
Biochemistry. 32:11977-11984
Kanamycin nucleotidyltransferase, as originally isolated from Staphylococcus aureus, inactivates the antibiotic kanamycin by catalyzing the transfer of a nucleotidyl group from nucleoside triphosphates such as ATP to the 4'-hydroxyl group of the amin
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::44f83d4336fd616c638b27080cbc48e0
https://doi.org/10.1021/bi00096a006
https://doi.org/10.1021/bi00096a006
Autor:
Hans H. Liao, Agnes M. Kanikula
Publikováno v:
Current Microbiology. 21:301-306
Recombinant plasmids carrying either the wildtype kanamycin nucleotidyltransferase gene encoded originally by the mesophilic plasmid pUB110 or the gene encoding the thermostable TK101 mutant were constructed and introduced intoBacillus stearothermoph
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::f6687629d4bc368c55dd445d17537c4b
https://doi.org/10.1007/bf02092095
https://doi.org/10.1007/bf02092095
Publikováno v:
Archives of biochemistry and biophysics. 295(1)
A thermostable mutant of kanamycin nucleotidyltransferase isolated by cloning and selection for kanamycin resistance in Bacillus stearothermophilus at 70 °C has been crystallized in a form suitable for high-resolution diffraction analysis. This enzy
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2a2805b3d481a9a0778e64df6ad8ef27
https://pubmed.ncbi.nlm.nih.gov/1315501
https://pubmed.ncbi.nlm.nih.gov/1315501