Zobrazeno 1 - 10
of 38
pro vyhledávání: '"Agnes K. Fok"'
Publikováno v:
European Journal of Cell Biology. 90:81-92
In Paramecium multimicronucleatum, the discoidal vesicles, the acidosomes and the 100-nm carrier vesicles are involved in phagosome formation, phagosome acidification and endosomal processing, respectively. Numerous cross bridges link these vesicles
Publikováno v:
Journal of Eukaryotic Microbiology. 55:481-491
In ciliates, calmodulin (CaM), as in other cells, has multiple functions, such as activation of regulatory enzymes and modulating calcium-dependent cellular processes. By immunogold localization, CaM is concentrated at multiple sites in Paramecium. I
Autor:
Richard D. Allen, Kiyoshi Yamauchi, Agnes K. Fok, Akinori Ishihara, Marilynn S. Aihara, Masaki Ishida
Publikováno v:
The Journal of Eukaryotic Microbiology. 49:185-196
Previous studies have shown that the vacuolar-ATPase (V-ATPase) of the contractile vacuole complexes (CVCs) in Paramecium multimicronucleatum is necessary for fluid segregation and osmoregulation. In the current study, immunofluorescence showed that
Publikováno v:
Molecular Biology of the Cell. 10:1031-1041
An mAb was raised to the C5 phagosomal antigen in Paramecium multimicronucleatum. To determine its function, the cDNA and genomic DNA encoding C5 were cloned. This antigen consisted of 315 amino acid residues with a predicted molecular weight of 36,5
Publikováno v:
Protoplasma. 196:12-20
Although it is generally accepted that phagosome acidification is induced through the activity of a vacuolar proton pump (V-ATPase) present on the phagosome membrane, exactly how these pumps are delivered to the phagosomes is not well understood. To
Publikováno v:
European Journal of Protistology. 32:25-31
Summary A monoclonal antibody, mAb MA-1 raised against the macronuclei of the ciliate Paramecium caudatum, has been shown to react with macronuclear nucleoli by cryo-immunoelectron microscopy. Immunoblots of two-dimensional sodium dodecylsulfate Poly
Publikováno v:
Cell Motility and the Cytoskeleton. 29:215-224
In an earlier study we reported the isolation of a cytoplasmic dynein from the cytosol of Paramecium multimicronucleatum. In this study we report the isolation and characterization of two cytosolic axonemal dyneins (22S and 12S) as well as a 19S cyto
Publikováno v:
Journal of Cell Science. 106:411-422
Acidosomes are organelles that in Paramecium are responsible for the acidification of phagosomes before phagosomes fuse with lysosomes. Using a combination of (a) the quick-freeze deep-etch (QF-DE) technique, (b) monoclonal antibodies (mAbs) that lab
Publikováno v:
European Journal of Protistology. 25:361-368
We report the use of nile red as a rapid and inexpensive method to estimate cellular lipids in three species of Paramecium and in Tetrahymena by the direct application of the dye to living or fixed cells without extraction and purification. Qualitati
Publikováno v:
The Journal of eukaryotic microbiology. 48(6)
The roles of somatic and oral cilia and solid particles during digestive vacuole (DV) formation in Paramecium multimicronucleatum were investigated using video-enhanced and immunofluorescence microscopy. Membrane incorporation into DVs was found to i