Excess of guide RNA reduces knockin efficiency and drastically increases on-target large deletions

Autor: Vanessa Chenouard, Isabelle Leray, Laurent Tesson, Severine Remy, Alasdair Allan, Daniel Archer, Adam Caulder, Agnès Fortun, Karine Bernardeau, Yacine Cherifi, Lydia Teboul, Laurent David, Ignacio Anegon

Publikováno v: iScience, Vol 26, Iss 4, Pp 106399- (2023)

Summary: CRISPR-Cas9 cleavage efficacy and accuracy are the main challenges gene editing faces, and they are particularly affected by the optimal formation of the ribonucleoprotein (RNP) complex. We used nano differential scanning fluorimetry, a labe

Externí odkaz: https://doaj.org/article/3e4821e8ad1840e39504e69a7b2227d8

A novel and efficient approach to high-throughput production of HLA-E/peptide monomer for T-cell epitope screening

Autor: Juliette Vaurs, Gaël Douchin, Klara Echasserieau, Romain Oger, Nicolas Jouand, Agnès Fortun, Leslie Hesnard, Mikaël Croyal, Frédéric Pecorari, Nadine Gervois, Karine Bernardeau

Publikováno v: Scientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)

Abstract Over the past two decades, there has been a great interest in the study of HLA-E-restricted αβ T cells during bacterial and viral infections, including recently SARS-CoV-2 infection. Phenotyping of these specific HLA-E-restricted T cells r

Externí odkaz: https://doaj.org/article/890ce0d044f14d9ebfb2c8235af6b09b

hnRNP‐A1 binds to the IRES of MELOE‐1 antigen to promote MELOE‐1 translation in stressed melanoma cells

Autor: Emmanuelle Com, François Lang, Charles Pineau, Catherine Rabu, Nathalie Labarrière, Agnès Fortun, Emilie Dupré, Floriane Briand, Maud Charpentier, Mike Maillasson

Publikováno v: Molecular Oncology
Molecular Oncology, Vol 16, Iss 3, Pp 594-606 (2022)

The major challenge in antigen‐specific immunotherapy of cancer is to select the most relevant tumor antigens to target. To this aim, understanding their mode of expression by tumor cells is critical. We previously identified a melanoma‐specific

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ef847be4bb06d0411c5a15c1ed3639e7
https://doi.org/10.1002/1878-0261.13088

Author response for 'hnRNP‐A1 binds to the IRES of MELOE‐1 antigen to promote its translation in stressed melanoma cells'

Autor: Agnès Fortun, Emilie Dupré, Nathalie Labarrière, Emmanuelle Com, François Lang, Charles Pineau, Floriane Briand, Catherine Rabu, Mike Maillasson, Maud Charpentier

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::63dfc4cbbf573bfb29f82c0b5bd48ecf
https://doi.org/10.1002/1878-0261.13088/v2/response1

Cancer vaccines: designing artificial synthetic long peptides to improve presentation of class I and class II T cell epitopes by dendritic cells

Autor: Léa Paolini, Olivier Adotevi, Maud Charpentier, E Dupel, Agnès Fortun, Emilie Dupré, Catherine Rabu, Nathalie Labarrière, Céline Beauvillain, L. Rangan, Jean-Baptiste Latouche, François Lang, Laetitia Florenceau

Publikováno v: OncoImmunology
OncoImmunology, Taylor & Francis, 2019, 8 (4), pp.e1560919. ⟨10.1080/2162402X.2018.1560919⟩
OncoImmunology, Taylor & Francis, 2019, pp.e1560919-2. ⟨10.1080/2162402x.2018.1560919⟩
Oncoimmunology
OncoImmunology, Vol 8, Iss 4 (2019)
OncoImmunology, 2019, pp.e1560919-2. ⟨10.1080/2162402x.2018.1560919⟩

International audience; There is now a consensus that efficient peptide vaccination against cancer requires that peptides should (i) be exclusively presented by professional APC and (ii) stimulate both CD4 and CD8-specific T cell responses. To this a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bbd5c6d1e4eb958168d0931139ddca4f
https://hal.archives-ouvertes.fr/hal-02131764/document