Robust detection of translocations in lymphoma FFPE samples using targeted locus capture-based sequencing

Autor: Joost Vermaat, Tom van Wezel, Paula J P de Vree, Wouter de Laat, Bauke Ylstra, Amin Allahyar, Marieke Simonis, Harma Feitsma, Adrien S. J. Melquiond, Max van Min, Agata Rakszewska, Erik Splinter, Daphne de Jong, Joost Swennenhuis, Milan Sharma, Mehmet Yilmaz, Arjan Diepstra, Roos J Leguit, Robert van der Geize, Phylicia Stathi, Karima Hajo, Nathalie J. Hijmering, Mark Pieterse, Marjon J.A.M. Verstegen, Peter H.L. Krijger, Ruud W J Meijers, G Tjitske Los-de Vries, Léon C van Kempen, Arjen H.G. Cleven

Publikováno v: Nature Communications, 12(1):3361. Nature Publishing Group
Nature Communications
Nature Communications, 12(1). Nature Publishing Group
Nature Communications, 12(1). NATURE PORTFOLIO
Nature Communications, Vol 12, Iss 1, Pp 1-15 (2021)
Allahyar, A, Pieterse, M, Swennenhuis, J, Los-de Vries, G T, Yilmaz, M, Leguit, R, Meijers, R W J, van der Geize, R, Vermaat, J, Cleven, A, van Wezel, T, Diepstra, A, van Kempen, L O C, Hijmering, N J, Stathi, P, Sharma, M, Melquiond, A S J, de Vree, P J P, Verstegen, M J A M, Krijger, P H L, Hajo, K, Simonis, M, Rakszewska, A, van Min, M, de Jong, D, Ylstra, B, Feitsma, H, Splinter, E & de Laat, W 2021, ' Robust detection of translocations in lymphoma FFPE samples using targeted locus capture-based sequencing ', Nature Communications, vol. 12, no. 1, 3361 . https://doi.org/10.1038/s41467-021-23695-8

In routine diagnostic pathology, cancer biopsies are preserved by formalin-fixed, paraffin-embedding (FFPE) procedures for examination of (intra-) cellular morphology. Such procedures inadvertently induce DNA fragmentation, which compromises sequenci

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f015170cc76e934c99a06da52084f3ba
https://research.vumc.nl/en/publications/dbd7c41c-2950-4947-b268-32207673d8fd

Quantitative Single-Cell mRNA Analysis in Hydrogel Beads

Autor: Rosa J. Stolper, Aigars Piruska, Agata Rakszewska, Wilhelm T. S. Huck, Anna B. Kolasa

Publikováno v: Angewandte Chemie. International Edition, 55, 6697-6700
Angewandte Chemie. International Edition, 55, 23, pp. 6697-6700

In recent years, technologies capable of analyzing single cells have emerged that are transforming many fields of biological research. Herein we report how DNA-functionalized hydrogel beads can serve as a matrix to capture mRNA from lysed single cell

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2599dcad62ba96477e9d391532e733d0
http://hdl.handle.net/2066/162021

Iterative operations on microdroplets and continuous monitoring of processes within them; determination of solubility diagrams of proteins

Autor: Slawomir Jakiela, Monika E. Dolega, Michal Razew, Agata Rakszewska, Olgierd Cybulski, Piotr Garstecki

Publikováno v: Scopus-Elsevier
Lab on a Chip

We demonstrate a technique for controlling the content of multiple microdroplets in time. We use this system to rapidly and quantiatively determine the solubility diagrams of two model proteins (lysozyme and ribonuclease A).

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dd89c33457ebeafe9b1e0e0f7bcc0288
http://www.scopus.com/inward/record.url?eid=2-s2.0-84873739257&partnerID=MN8TOARS