Agrobacterium-mediated in planta transformation of cut coleoptile: a new, simplified, and tissue culture-independent method to deliver the CRISPR/Cas9 system in rice.

Autor: Tamizi AA; Department of Plant Science, Kulliyyah of Science, International Islamic University Malaysia (IIUM), 25200, Kuantan, Pahang, Malaysia.; Biotechnology and Nanotechnology Research Centre, Malaysian Agricultural Research and Development Institute (MARDI), 43400, Serdang, Selangor, Malaysia., Md-Yusof AA; Department of Plant Science, Kulliyyah of Science, International Islamic University Malaysia (IIUM), 25200, Kuantan, Pahang, Malaysia., Mohd-Zim NA; Department of Plant Science, Kulliyyah of Science, International Islamic University Malaysia (IIUM), 25200, Kuantan, Pahang, Malaysia.; FGV R&D Sdn. Bhd, FGV Innovation Centre, PT 23417 Lengkuk Teknologi, 71760, Bandar Enstek, Negeri Sembilan, Malaysia., Nazaruddin NH; Biotechnology and Nanotechnology Research Centre, Malaysian Agricultural Research and Development Institute (MARDI), 43400, Serdang, Selangor, Malaysia., Sekeli R; Biotechnology and Nanotechnology Research Centre, Malaysian Agricultural Research and Development Institute (MARDI), 43400, Serdang, Selangor, Malaysia. lynn@mardi.gov.my., Zainuddin Z; Department of Plant Science, Kulliyyah of Science, International Islamic University Malaysia (IIUM), 25200, Kuantan, Pahang, Malaysia.; Plant Productivity and Sustainable Resource Unit, Department of Plant Science, Kulliyyah of Science, International Islamic University Malaysia (IIUM), 25200, Kuantan, Pahang, Malaysia., Samsulrizal NH; Department of Plant Science, Kulliyyah of Science, International Islamic University Malaysia (IIUM), 25200, Kuantan, Pahang, Malaysia. hidayahsamsulrizal@iium.edu.my.; Plant Productivity and Sustainable Resource Unit, Department of Plant Science, Kulliyyah of Science, International Islamic University Malaysia (IIUM), 25200, Kuantan, Pahang, Malaysia. hidayahsamsulrizal@iium.edu.my.

Publikováno v: Molecular biology reports [Mol Biol Rep] 2023 Nov; Vol. 50 (11), pp. 9353-9366. Date of Electronic Publication: 2023 Oct 11.