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Akademický článek

Discovery of APL-1030, a Novel, High-Affinity Nanofitin Inhibitor of C3-Mediated Complement Activation

Autor: Joshua Garlich, Mathieu Cinier, Anne Chevrel, Anaëlle Perrocheau, David J. Eyerman, Mark Orme, Olivier Kitten, Lukas Scheibler

Publikováno v: Biomolecules, Vol 12, Iss 3, p 432 (2022)

Uncontrolled complement activation contributes to multiple immune pathologies. Although synthetic compstatin derivatives targeting C3 and C3b are robust inhibitors of complement activation, their physicochemical and molecular properties may limit acc

Externí odkaz: https://doaj.org/article/7819b32d28f843e9ba972b23178a3ce0

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Akademický článek

Artificial Affinity Proteins as Ligands of Immunoglobulins

Autor: Barbara Mouratou, Ghislaine Béhar, Frédéric Pecorari

Publikováno v: Biomolecules, Vol 5, Iss 1, Pp 60-75 (2015)

A number of natural proteins are known to have affinity and specificity for immunoglobulins. Some of them are widely used as reagents for detection or capture applications, such as Protein G and Protein A. However, these natural proteins have a defin

Externí odkaz: https://doaj.org/article/17597f96ac624438b4d9e654e1776edb

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Evaluation of docking procedures reliability in affitins-partners interactions

Autor: Anna Ranaudo, Ugo Cosentino, Claudio Greco, Giorgio Moro, Alessandro Bonardi, Alessandro Maiocchi, Elisabetta Moroni

Publikováno v: Frontiers in chemistry. 10

Affitins constitute a class of small proteins belonging to Sul7d family, which, in microorganisms such as Sulfolobus acidocaldarius, bind DNA preventing its denaturation. Thanks to their stability and small size (60–66 residues in length) they have

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::092161b256f5bd13504649acb491d94b
https://pubmed.ncbi.nlm.nih.gov/36531315

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Whole‐bacterium ribosome display selection for isolation of highly specific anti‐ Staphyloccocus aureus Affitins for detection‐ and capture‐based biomedical applications

Autor: Stanimir Kambarev, Ghislaine Béhar, Frédéric Pecorari, Axelle Renodon-Cornière, Petar Vukojicic, Stéphane Corvec, Nathalie Caroff, Barbara Mouratou

Publikováno v: Biotechnology and Bioengineering
Biotechnology and Bioengineering, 2019, Epub ahead of print. ⟨10.1002/bit.26989⟩
Biotechnology and Bioengineering, Wiley, 2019, Epub ahead of print. ⟨10.1002/bit.26989⟩

International audience; Detection and capture methods using antibodies have been developed to ensure identification of pathogens in biological samples. Though antibodies have many attractive properties, they also have limitations and there are needs

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::caebd519a6e1c8d40adcbc869d66ade3
https://doi.org/10.1002/bit.26989

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Akademický článek

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A novel, smaller scaffold for Affitins: Showcase with binders specific for EpCAM

Autor: Valentina Kalichuk, Véronique Préat, Gonzalo Obal, Frédéric Pecorari, Mike Maillasson, Federico Carrión, Barbara Mouratou, Ghislaine Béhar, Axelle Renodon-Cornière

Publikováno v: Biotechnology and Bioengineering
Biotechnology and Bioengineering, Wiley, 2017, Epub ahead of print. ⟨10.1002/bit.26463⟩
Biotechnology and Bioengineering, 2017, Epub ahead of print. ⟨10.1002/bit.26463⟩

International audience; Affitins are highly stable engineered affinity proteins, originally derived from Sac7d and Sso7d, two 7 kDa DNA-binding polypeptides from Sulfolobus genera. Their efficiency as reagents for intracellular targeting, enzyme inhi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cc3faee1c79e143246d3a00a336eb44d
https://www.hal.inserm.fr/inserm-01629637/file/KalichukEq13.pdf

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Switching an anti-IgG binding site between archaeal extremophilic proteins results in Affitins with enhanced pH stability

Autor: Ghislaine Béhar, Sabino Pacheco, Barbara Mouratou, Mike Maillasson, Frédéric Pecorari

Publikováno v: Journal of Biotechnology. 192:123-129

As a useful reagent for biotechnological applications, a scaffold protein needs to be as stable as possible to ensure longer lifetimes. We have developed archaeal extremophilic proteins from the “7 kDa DNA-binding” family as scaffolds to derive a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bc94eae309e985fc398188bc173579c9
https://doi.org/10.1016/j.jbiotec.2014.10.006

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Affitins for protein purification by affinity magnetic fishing

Autor: A. Cecília A. Roque, Raquel Santos, Frédéric Pecorari, Aline Canani Viecinski, Barbara Mouratou, Stella Ottengy, Ghislaine Béhar, Cláudia S. M. Fernandes

Publikováno v: Journal of Chromatography A
Journal of Chromatography A, Elsevier, 2016, 1457, pp.50-8. ⟨10.1016/j.chroma.2016.06.020⟩
Journal of Chromatography A, 2016, 1457, pp.50-8. ⟨10.1016/j.chroma.2016.06.020⟩

International audience; Currently most economical and technological bottlenecks in protein production are placed in the downstream processes. With the aim of increasing the efficiency and reducing the associated costs, various affinity ligands have b

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::85a2d44afae64ba83f9eedadfd445ff7
https://www.hal.inserm.fr/inserm-01342446/file/ArticlePecorari2016.pdf

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Affitins as robust tailored reagents for affinity chromatography purification of antibodies and non-immunoglobulin proteins

Autor: Barbara Mouratou, Frédéric Pecorari, Ghislaine Béhar, Axelle Renodon-Cornière

Publikováno v: Journal of Chromatography A
Journal of Chromatography A, Elsevier, 2016, 1441, pp.44-51. ⟨10.1016/j.chroma.2016.02.068⟩
Journal of Chromatography A, 2016, 1441, pp.44-51. ⟨10.1016/j.chroma.2016.02.068⟩

International audience; Affinity chromatography is a convenient way of purifying proteins, as a high degree of purity can be reached in one step. The use of tags has greatly contributed to the popularity of this technique. However, the addition of ta

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::637f535bc1d3905f5a58f81ee31f45c2
https://www.hal.inserm.fr/inserm-01292506

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