Extended disorder at the cell surface: The conformational landscape of the ectodomains of syndecans

Autor: Frank Gondelaud, Fabien Chirot, Adam Liwo, Sylvie Ricard-Blum, Aurélien Le Fèvre, Bertrand Duclos, Adriana E. Miele, Mathilde Bouakil

Publikováno v: Matrix Biology Plus
Matrix Biology Plus, 2021, 12 (3-4), pp.100081. ⟨10.1016/j.mbplus.2021.100081⟩
'Matrix Biology Plus ', vol: 12, pages: 100081-1-100081-19 (2021)
Matrix Biology Plus, Vol 12, Iss, Pp 100081-(2021)

Highlights • The syndecan ectodomains are flexible random-coil intrinsically disordered proteins. • Their extended conformations have the same size as their cell surface partners. • Their binding-motifs to cells and receptors are not accessible

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ffdf479eba653d09f2444a8e356f8047
https://doi.org/10.1016/j.mbplus.2021.100081

A comparative analysis of secreted protein disulfide isomerases from the tropical co-endemic parasites Schistosoma mansoni and Leishmania major

Autor: Giovanna Boumis, Romain Salza, Silvia Chichiarelli, Lorenzo Maugliani, Sylvie Ricard-Blum, Bertrand Duclos, Sofiane Badaoui, Adriana E. Miele

Publikováno v: Scientific Reports
Scientific Reports, Vol 9, Iss 1, Pp 1-12 (2019)
'Scientific Reports ', vol: 9, pages: 9568-1-9568-12 (2019)
Scientific reports 9(1), 9568 (2019). doi:10.1038/s41598-019-45709-8

Scientific reports 9(1), 9568 (2019). doi:10.1038/s41598-019-45709-8
The human parasites Schistosoma mansoni and Leishmania major are co-endemic and a major threat to human health. Though displaying different tissue tropisms, they excrete/secret

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::20456cc9282c738982c32759bce80a78
http://hdl.handle.net/11573/1295312

The oligomeric assembly of the phosphodiesterase-5 is a mixture of dimers and tetramers: A putative role in the regulation of function

Autor: Francesco Malatesta, Stefano Biagioni, Silvia Cardarelli, Michele Saliola, Adriana E. Miele, Fabio Naro, Carlotta Zamparelli, Mauro Giorgi

Publikováno v: Biochimica et Biophysica Acta (BBA)-General Subjects
Biochimica et Biophysica Acta (BBA)-General Subjects, Elsevier, 2018, 1862 (10), pp.2183-2190. ⟨10.1016/j.bbagen.2018.07.010⟩

Background Phosphodiesterases (PDEs) are a superfamily of evolutionary conserved cyclic nucleotides (cAMP/cGMP) hydrolysing enzymes, components of transduction pathways regulating crucial aspects of cell life. PDE5, one of these families, is the mole

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aec8cafcd98648ddeb3766706783df94
https://pubmed.ncbi.nlm.nih.gov/30025857

Insights into the structure and dynamics of lysyl oxidase propeptide, a flexible protein with numerous partners

Autor: Sylvain D. Vallet, Adriana E. Miele, Urszula Uciechowska-Kaczmarzyk, Adam Liwo, Bertrand Duclos, Sergey A. Samsonov, Sylvie Ricard-Blum

Publikováno v: Scientific Reports
Scientific Reports, Nature Publishing Group, 2018, 8 (1), pp.122-149. ⟨10.1038/s41598-018-30190-6⟩
Scientific Reports, Vol 8, Iss 1, Pp 1-16 (2018)

International audience; Lysyl oxidase (LOX) catalyzes the oxidative deamination of lysine and hydroxylysine residues in collagens and elastin, which is the first step of the cross-linking of these extracellular matrix proteins. It is secreted as a pr

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::7b459f52d5c2389a7ece105059560da3

Protein Engineering of Multi-Modular Transcription Factor Alcohol Dehydrogenase Repressor 1 (Adr1p), a Tool for Dissecting In Vitro Transcription Activation

Autor: Daniele Frascaria, Veronica Morea, Adriana E. Miele, M Buttinelli, Gianna Panetta, Ambra Bucci

Publikováno v: Biomolecules
Biomolecules, Vol 9, Iss 9, p 497 (2019)
Biomolecules 9 (2019): 497. doi:10.3390/biom9090497
info:cnr-pdr/source/autori:Buttinelli, Memmo; Panetta, Gianna; Bucci, Ambra; Frascaria, Daniele; Morea, Veronica; Miele, Adriana Erica/titolo:Protein Engineering of Multi-Modular Transcription Factor Alcohol Dehydrogenase Repressor 1 (Adr1p), a Tool for Dissecting In Vitro Transcription Activation/doi:10.3390%2Fbiom9090497/rivista:Biomolecules/anno:2019/pagina_da:497/pagina_a:/intervallo_pagine:497/volume:9

Studying transcription machinery assembly in vitro is challenging because of long intrinsically disordered regions present within the multi-modular transcription factors. One example is alcohol dehydrogenase repressor 1 (Adr1p) from fermenting yeast,

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5ccc1212e63b43d148046bda08682d24
https://doi.org/10.3390/biom9090497