Výsledky vyhledávání - "Adrian Sanden"

Modifying an ÄKTApurifier System for the Automated Acquisition of Samples for Kinetic Modeling of Batch Reactions

Autor: Jürgen Hubbuch, Adrian Sanden, Sandra Haas

Publikováno v: SLAS Technology. 25:106-110

Recording the data necessary to assess the kinetics of a reaction can be labor-intensive. In this technology brief, we show a method to automate this task by utilizing parts of an ÄKTApurifier chromatography system to automatically take samples from

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f2bf26c8289acb1dd7c708f548dd6cdd
https://doi.org/10.1177/2472630319891976

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Fourier-transform infrared spectroscopy as a process analytical technology for near real time in-line estimation of the degree of PEGylation in chromatography

Autor: Matthias Rüdt, Susanna Suhm, Adrian Sanden, Jürgen Hubbuch

Publikováno v: Journal of Chromatography A. 1608:460410

PEGylation of biological macromolecules is a well-established strategy to increase circulation half-life, decrease renal clearance and improve biocompatibility. PEGylation is a process in which polyethylene glycol (PEG) is covalently attached to a ta

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3b9eb8ce6421e5bfc34004e661a4a07d
https://doi.org/10.1016/j.chroma.2019.460410

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Advances in inline quantification of co-eluting proteins in chromatography: Process-data-based model calibration and application towards real-life separation issues

Autor: Adrian Sanden, Nina Brestrich, Joseph Bertolini, Axel Kraft, Jürgen Hubbuch, Karl B. McCann

Publikováno v: Biotechnology and Bioengineering. 112:1406-1416

Pooling decisions in preparative liquid chromatography for protein purification are usually based on univariate UV absorption measurements that are not able to differentiate between product and co-eluting contaminants. This can result in inconsistent

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::9e949e0aa168022a6afea738af112498
https://doi.org/10.1002/bit.25546

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In-line Fourier-transform infrared spectroscopy as a versatile process analytical technology for preparative protein chromatography

Autor: Josefine Morgenstern, Nina Brestrich, Matthias Rüdt, Adrian Sanden, Stefan Heissler, Steffen Großhans, Jürgen Hubbuch

Publikováno v: Publons
Journal of Chromatography A
Journal of chromatography / A, 1547, 37–44

Fourier-transform infrared spectroscopy (FTIR) is a well-established spectroscopic method in the analysis of small molecules and protein secondary structure. However, FTIR is not commonly applied for in-line monitoring of protein chromatography. Here

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d8ef58990518ec68d1aa2c50ea6f2ec2
https://pubmed.ncbi.nlm.nih.gov/29530404

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Advances in inline quantification of co-eluting proteins in chromatography: Process-data-based model calibration and application towards real-life separation issues

Autor: Nina, Brestrich, Adrian, Sanden, Axel, Kraft, Karl, McCann, Joseph, Bertolini, Jürgen, Hubbuch

Publikováno v: Biotechnology and bioengineering. 112(7)

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::0fa225cf14eff666f9f6997b0331a59d
https://pubmed.ncbi.nlm.nih.gov/25683378

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