De novo active sites for resurrected Precambrian enzymes

Autor: Valeria A. Risso, Sergio Martinez-Rodriguez, Adela M. Candel, Dennis M. Krüger, David Pantoja-Uceda, Mariano Ortega-Muñoz, Francisco Santoyo-Gonzalez, Eric A. Gaucher, Shina C. L. Kamerlin, Marta Bruix, Jose A. Gavira, Jose M. Sanchez-Ruiz

Publikováno v: Nature Communications, Vol 8, Iss 1, Pp 1-13 (2017)

The emergence of novel catalytic functions in ancient proteins likely played a role in the evolution of modern enzymes. Here, the authors use protein sequences from Precambrian beta-lactamases and demonstrate that a single hydrophobic-to-ionizable am

Externí odkaz: https://doaj.org/article/7c76aaa815614d3a948e230f4b0670e6

Phage display identification of nanomolar ligands for human NEDD4-WW3: Energetic and dynamic implications for the development of broad-spectrum antivirals

Autor: Francisco Castillo, Carles Corbi-Verge, Javier Murciano-Calles, Adela M. Candel, Ziying Han, Manuel Iglesias-Bexiga, Javier Ruiz-Sanz, Philip M. Kim, Ronald N. Harty, Jose C. Martinez, Irene Luque

Publikováno v: Digibug. Repositorio Institucional de la Universidad de Granada
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This research has been financed by grants BIO2016-78746-C2-1-R and PID2020-112895RB-100 from the Spanish Ministry of Education and Science (I.L.) including AEI/FEDER EU funds. R.N.H. was funded in part by National Institutes of Health grants AI138052

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1877c791c8c0c7dec7de3cd2d1417ad5
https://doi.org/10.1016/j.ijbiomac.2022.03.010

A comparison of the location in membranes of curcumin and curcumin-derived bivalent compounds with potential neuroprotective capacity for Alzheimer’s disease

Autor: Liu He, Andrea Beltrán, Juan C. Gómez-Fernández, Yuqi Jiang, Alessio Ausili, Adela M. Candel, Shijun Zhang, Alejandro Torrecillas, Victoria Gomez-Murcia, José A. Teruel

Publikováno v: Colloids Surf B Biointerfaces

Curcumin and two bivalent compounds, namely 17MD and 21MO, both obtained by conjugation of curcumin with a steroid molecule that acts as a membrane anchor, were comparatively studied. When incorporated into 1,2-dipalmitoyl-sn-glycero-3-phosphocholine

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::22189d383356c8e65834e24620d830e3
https://doi.org/10.1016/j.colsurfb.2020.111525

De novo active sites for resurrected Precambrian enzymes

Autor: Marta Bruix, Francisco Santoyo-Gonzalez, David Pantoja-Uceda, Mariano Ortega-Muñoz, Jose M. Sanchez-Ruiz, Shina Caroline Lynn Kamerlin, Sergio Martínez-Rodríguez, Valeria A. Risso, Dennis M. Krüger, Jose A. Gavira, Adela M. Candel, Eric A. Gaucher

Publikováno v: Nature Communications, Vol 8, Iss 1, Pp 1-13 (2017)
Digital.CSIC. Repositorio Institucional del CSIC
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Nature Communications
'Nature Communications ', vol: 8, pages: 16113-1-16113-13 (2017)

Protein engineering studies often suggest the emergence of completely new enzyme functionalities to be highly improbable. However, enzymes likely catalysed many different reactions already in the last universal common ancestor. Mechanisms for the eme

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4ee0937e9d229b9332b6f0323f5d02c1
http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-331231

An Error Analysis for Two-State Protein-Folding Kinetic Parameters and φ-Values: Progress toward Precision by Exploring pH Dependencies on Leffler Plots

Autor: Eva S. Cobos, Jose C. Martinez, Adela M. Candel

Publikováno v: Biophysical Journal. 94(11):4393-4404

The interpretation of phi-values has led to an understanding of the folding transition state ensemble of a variety of proteins. Although the main guidelines and equations for calculating phi are well established, there remains some controversy about

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fc3db16716b08b24ab5d63249f30b5b9