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Akademický článek

InterOpt: Improved gene expression quantification in qPCR experiments using weighted aggregation of reference genes

Autor: Adel Salimi, Saeid Rahmani, Ali Sharifi-Zarchi

Publikováno v: iScience, Vol 26, Iss 10, Pp 107945- (2023)

Summary: qPCR is still the gold standard for gene expression quantification. However, its accuracy is highly dependent on the normalization procedure. The conventional method involves using the geometric mean of multiple study-specific reference gene

Externí odkaz: https://doaj.org/article/cd3f716f94cc40ed9934e94d0bd00c59

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Akademický článek

miR-361-5p as a promising qRT-PCR internal control for tumor and normal breast tissues.

Autor: Sogol Ghanbari, Adel Salimi, Saeid Rahmani, Nahid Nafissi, Ali Sharifi-Zarchi, Seyed Javad Mowla

Publikováno v: PLoS ONE, Vol 16, Iss 6, p e0253009 (2021)

BackgroundOne of the most widely used evaluation methods in miRNA experiments is qRT-PCR. However, selecting suitable internal controls (IC) is crucial for qRT-PCR experiments. Currently, there is no consensus on the ICs for miRNA qRT-PCR experiments

Externí odkaz: https://doaj.org/article/75b4965c643b47c7a4e3ab2f9f8123ad

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A panel of four miRNAs (miR-190b, miR-584-5p, miR-452-5p, and miR-1306-5p) is capable of classifying luminal and non-luminal breast cancers

Autor: Faranak Farahmand, Saied Rahmani, Hadi Bayat, Adel Salimi, Sogol Ghanbari, Afsaneh Malekzadeh Shafaroudi, Ali Sharifi-Zarchi, Mohammad Vasei, Seyed-Javad Mowla

BACKGROUNDIdentifying the molecular subtypes of breast cancer (BC) plays a crucial role in enhancing the efficacy of therapy. MiRNAs (miRs) with differential expressions in different subtypes of breast tumors can be considered as non-invasive biomark

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::6359f5d07ec24a09272f490fa88377b4
https://doi.org/10.1101/2022.10.18.22281125

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miR-361-5p as a promising qRT-PCR internal control for tumor and normal breast tissues

Autor: Ali Sharifi-Zarchi, Seyed Javad Mowla, Saeid Rahmani, Sogol Ghanbari, Nahid Nafissi, Adel Salimi

Publikováno v: PLoS ONE
PLoS ONE, Vol 16, Iss 6, p e0253009 (2021)

Background One of the most widely used evaluation methods in miRNA experiments is qRT-PCR. However, selecting suitable internal controls (IC) is crucial for qRT-PCR experiments. Currently, there is no consensus on the ICs for miRNA qRT-PCR experiment

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b1216bcec60f806dce6df72a66fe5fef
https://doi.org/10.1371/journal.pone.0253009

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