Zobrazeno 1 - 10
of 46
pro vyhledávání: '"Adam Zweifach"'
Autor:
David Barrett, Meghan Wyatt, Haim Bar, Mark K. Haynes, Bruce S. Edwards, Larry A. Sklar, Adam Zweifach
Publikováno v:
SLAS Discovery, Vol 27, Iss 5, Pp 314-322 (2022)
The solid tumor microenvironment (TME) suppresses immune responses. Three alterations in the TME converge on a pathway triggered by elevated cyclic AMP (cAMP) that suppresses T cell receptor (TCR) signaling. We developed a phenotypic assay to screen
Externí odkaz:
https://doaj.org/article/cd239189b37c4c4296a91e5340d47196
Autor:
Adam Zweifach
Publikováno v:
SLAS Discovery, Vol 28, Iss 2, Pp 1-2 (2023)
Externí odkaz:
https://doaj.org/article/17df1303525d468996bbe14cc1bdd68a
Autor:
Adam Zweifach, Haim Bar
Publikováno v:
SLAS Discovery. 25:1000-1008
The assay metric Z' has come to play a critical gatekeeping role in determining whether high-throughput assays can be performed. While Z' is commonly required to be > 0.5, this expectation is not well supported. Requiring Z' > 0.5 likely prevents man
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b2711942e15d4b2c50130080b6055f8d
https://doi.org/10.1177/2472555220942764
https://doi.org/10.1177/2472555220942764
Autor:
Mark W. Peczuh, Santosh Keshipeddy, Chris Lorenc, Dennis L. Wright, Liza Henowitz, Victoria L. Robinson, Zachary P. Cannone, Ala M. Shaqra, Adam Zweifach
Publikováno v:
ACS Combinatorial Science. 21:192-197
Many small molecule natural products with antibiotic and antiproliferative activity are adorned with a carbohydrate residue as part of their molecular structure. The carbohydrate moiety can act to mediate key interactions with the target, attenuate p
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bf9793bb2cecf9ba19c809dcca040e09
https://doi.org/10.1021/acscombsci.8b00139
https://doi.org/10.1021/acscombsci.8b00139
Publikováno v:
SLAS Discovery. 23:907-918
Both glycolysis and mitochondrial energetics are targets of interest for developing antiproliferative cancer therapeutics. We developed a novel multiple-read assay based on long-term expression in K562 cells of a genetically encoded intramolecular F
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::144cb317c67dc9cafa41b42d8b73e9f1
https://doi.org/10.1177/2472555218780636
https://doi.org/10.1177/2472555218780636
Publikováno v:
SLAS Discovery. 23:646-655
We previously developed a flow cytometry assay that monitored lytic granule exocytosis in cytotoxic T lymphocytes stimulated by contacting beads coated with activating anti-CD3 antibodies. That assay was multiplexed in that responses of cells that di
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b63bcca26793cefbf5b6f0a53a5a9a78
https://doi.org/10.1177/2472555218777731
https://doi.org/10.1177/2472555218777731
Autor:
Adam Zweifach
Publikováno v:
SLAS discovery : advancing life sciences RD. 25(1)
Academic researchers looking for material to screen may benefit from plated compound collections provided at no cost except shipping by the U.S. National Cancer Institute (NCI). Four plated sets are available, two of which comprise diverse synthetic
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0cac3fc562685b3f3ba98617c35fe75c
https://pubmed.ncbi.nlm.nih.gov/31491345
https://pubmed.ncbi.nlm.nih.gov/31491345
Autor:
Xiuyi Alexander Yang, Adam Zweifach
Publikováno v:
SLAS discovery : advancing life sciences RD. 24(6)
Intramolecular CFP-YFP fluorescence resonance energy transfer (FRET) sensors expressed in cells are powerful research tools but have seen relatively little use in screening. We exploited the discovery that the expression of a CFP-YFP FRET diacylglyce
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dc20b770bc918db71508acc45961b0de
https://pubmed.ncbi.nlm.nih.gov/30802416
https://pubmed.ncbi.nlm.nih.gov/30802416
Autor:
Adam Zweifach, Melanie M. Barra, Jaimee Doucette, Marcy J. Balunas, Rory J. Geyer, Ziyan Zhao
Publikováno v:
SLAS Discovery. 21:535-547
Genetically encoded sensors based on intramolecular FRET between CFP and YFP are used extensively in cell biology research. Flow cytometry has been shown to offer a means to measure CFP-YFP FRET; we suspected it would provide a unique way to conduct
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::60419bfbdaed7dc47e564b282be88350
https://doi.org/10.1177/1087057116634007
https://doi.org/10.1177/1087057116634007
Autor:
Zachary Cannone, Ala Shaqra, Chris Lorenc, Liza Henowitz, Santosh Keshipeddy, Victoria Robinson, Adam Zweifach, Dennis Wright, Mark Peczuh
Many small molecule natural products with are adorned with a carbohydrate as part of their molecular structure that acts to mediate key interactions with the target, attenuate physicochemical properties, or both. Facile incorporation of a carbohydrat
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0e10fddd638b99df93f2c27e13c3f783
https://doi.org/10.26434/chemrxiv.7090667.v1
https://doi.org/10.26434/chemrxiv.7090667.v1
