Výsledky vyhledávání - "Adam S. Brank"

Optimization of Baculovirus-Mediated Expression and Purification of Hexahistidine-Tagged Murine DNA (Cytosine-C5)-Methyltransferase-1 in Spodoptera frugiperda 9 Cells

Autor: Judith K. Christman, Dana M. van Bemmel, Adam S. Brank

Publikováno v: Protein Expression and Purification. 25:31-40

Enzymatic DNA methylation of carbon 5 of cytosines is an epigenetic modification that plays a role in regulating gene expression, differentiation, and tumorigenesis. DNA (cytosine-C5)-methyltransferase-1 is the enzyme responsible for maintaining esta

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b95adef7be6e5ac5833e0498717ad2a6
https://doi.org/10.1006/prep.2001.1606

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Use of Oligodeoxyribonucleotides with Conformationally Constrained Abasic Sugar Targets To Probe the Mechanism of Base Flipping by HhaI DNA (Cytosine C5)-methyltransferase

Autor: Peiyuan Wang, Adam S. Brank, Marc C. Nicklaus, Alexander D. MacKerell, Victor E. Marquez, Nilesh K. Banavali, Judith K. Christman,‡ and

Publikováno v: Journal of the American Chemical Society. 122:12422-12434

X-ray crystallographic studies of HhaI DNA (cytosine-C5)-methyltransferase (M.HhaI) covalently linked to methylated 5-fluorocytosine in DNA provided the first direct evidence that the cytosine residue targeted for methylation was “flipped” out of

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::d813b16002e958b3a2665f7b5f00512b
https://doi.org/10.1021/ja001989s

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Mechanism of inhibition of DNA (cytosine C5)-methyltransferases by oligodeoxyribonucleotides containing 5,6-dihydro-5-azacytosine 1 1Edited by R. Huber

Autor: Janice R. Sufrin, Estela Alvarez, Canio J. Marasco, Victor E. Marquez, Xiaodong Cheng, Adam S. Brank, Margaret O'Gara, Harry Ford, Judith K. Christman, Amanda J. Goddard, Gholamreza Sheikhnejad

Publikováno v: Journal of Molecular Biology. 285:2021-2034

A key step in the predicted mechanism of enzymatic transfer of methyl groups from S-adenosyl- l -methionine (AdoMet) to cytosine residues in DNA is the transient formation of a dihydrocytosine intermediate covalently linked to cysteine in the active

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::4829e75f565ddb91ce3ae3f0c2544cb5
https://doi.org/10.1006/jmbi.1998.2426

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DNA (Cytosine-C5) methyltransferase inhibition by oligodeoxyribonucleotides containing 2-(1H)-pyrimidinone (zebularine aglycon) at the enzymatic target site

Autor: Dana M. van Bemmel, Adam S. Brank, Ramon Eritja, Victor E. Marquez, Judith K. Christman

Publikováno v: Digital.CSIC. Repositorio Institucional del CSIC
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20 pages, 7 figures, 1 table.-- PMID: 19467223 [PubMed].-- PMCID: PMC2756644.-- NIHMSID: NIHMS130041.-- Printed version published Sep 15, 2009.
Aberrant cytosine methylation in promoter regions leads to gene silencing associated with cancer prog

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::378cc0196ee1218ed2aacf2a7f973cb4
http://hdl.handle.net/10261/28601

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Synthesis of oligonucleotide inhibitors of DNA (Cytosine-C5) methyltransferase containing 5-azacytosine residues at specific sites

Autor: Victor E. Marquez, Ramon Eritja, Judith K. Christman, Adam S. Brank, Ramon G. Garcia

Publikováno v: Digital.CSIC. Repositorio Institucional del CSIC
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The incorporation of 5-azacytosine residues into DNA causes potent inhibition of DNA (Cytosine-C5) methyltransferases. The synthesis of oligodeoxyribonucleotides incorporating single or multiple 5-aza-2′-deoxycytidine residues at precise sites was

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a881ef28370670d6c4b3e8e055ea5391
https://pubmed.ncbi.nlm.nih.gov/11838638

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Inhibition of Hha I DNA (cytosine-c5) methyltransferase by oligodeoxyribonucleotides containing 5-Aza-2′-deoxycytidine: Examination of the intertwined roles of co-factor, target, transition state structure and enzyme conformation

Autor: Judith K. Christman, Adam S. Brank, Victor E. Marquez, Ramon Eritja, Ramon G. Garcia

Publikováno v: Digital.CSIC. Repositorio Institucional del CSIC
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The presence of 5-azacytosine (ZCyt) residues in DNA leads to potent inhibition of DNA (cytosine-C5) methyltranferases (C5-MTases) in vivo and in vitro. Enzymatic methylation of cytosine in mammalian DNA is an epigenetic modification that can alter g

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c93c15203bb72bc7a2d5fd9281cf94af
http://hdl.handle.net/10261/124720

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