Výsledky vyhledávání - "Adam M. McCoy"

Expression of the 22 nucleotide let-7 heterochronic RNA throughout the Metazoa: a role in life history evolution?

Autor: Emili Saló, Mark Q. Martindale, Jaume Baguñà, Adam M. McCoy, Gary Ruvkun, Eva Jimenez, Amy E. Pasquinelli

Publikováno v: Evolution and Development. 5:372-378

SUMMARY The22nucleotidelet-7smalltemporalRNAhasbeen found consistently in samples from diverse bilateria butnot from sponge or cnidarians. Here we further examine thephylogenetic distribution of this regulatory RNA by samplingrepresentativesofdiverse

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::df46f070e7e13c8e946442cd3f4f5890
https://doi.org/10.1046/j.1525-142x.2003.03044.x

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A droplet digital PCR detection method for rare L1 insertions in tumors

Autor: Adam M. McCoy, Joshua Fenrich, Vincent A Streva, Prescott L. Deininger, Travis B. White

Publikováno v: Mobile DNA

Background The active human mobile element, long interspersed element 1 (L1) currently populates human genomes in excess of 500,000 copies per haploid genome. Through its mobility via a process called target primed reverse transcription (TPRT), L1 mo

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7f89acb2069f155a6c6a761af8b6cb8c
https://doi.org/10.1186/s13100-014-0030-4

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Leptospiral Outer Membrane Proteins OmpL1 and LipL41 Exhibit Synergistic Immunoprotection

Autor: James Matsunaga, Mary K. Mazel, Garlo Chao, David A. Haake, Adam M. McCoy, Elizabeth A. Wagar, Frank Milward

Publikováno v: Infection and Immunity. 67:6572-6582

New vaccine strategies are needed for prevention of leptospirosis, a widespread human and veterinary disease caused by invasive spirochetes belonging to the genus Leptospira . We have examined the immunoprotective capacity of the leptospiral porin Om

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::65c946a690be7a1631b11caf8f22ff30
https://doi.org/10.1128/iai.67.12.6572-6582.1999

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Characterization of Leptospiral Outer Membrane Lipoprotein LipL36: Downregulation Associated with Late-Log-Phase Growth and Mammalian Infection

Autor: David A. Haake, Jay D. Pruetz, Ellen S. Shang, Carole A. Bolin, Mary K. Mazel, Carleen Martinich, Theresa A. Summers, Adam M. McCoy

Publikováno v: Infection and Immunity. 66:1579-1587

We report the cloning of the gene encoding a 36-kDa leptospiral outer membrane lipoprotein, designated LipL36. We obtained the N-terminal amino acid sequence of a staphylococcal V8 proteolytic-digest fragment in order to design an oligonucleotide pro

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c26c43e84018672304bdd34e7aa50f2b
https://doi.org/10.1128/iai.66.4.1579-1587.1998

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Heat shock response and groEL sequence of Bartonella henselae and Bartonella quintana

Autor: Theresa A. Summers, William A. Schwartzman, David A. Haake, Adam M. McCoy

Publikováno v: Microbiology. 143:2807-2815

Summary: Transmission of Bartonella species from ectoparasites to the mammalian host involves adaptation to thermal and other forms of stress. In order to better understand this process, the heat shock response of Bartonella henselae and Bartonella q

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https://doi.org/10.1099/00221287-143-8-2807

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Nephromyces, a beneficial apicomplexan symbiont in marine animals

Autor: Claudio H. Slamovits, Mary Beth Saffo, Christopher W. Rieken, Adam M. McCoy

With malaria parasites ( Plasmodium spp.), Toxoplasma , and many other species of medical and veterinary importance its iconic representatives, the protistan phylum Apicomplexa has long been defined as a group composed entirely of parasites and patho

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https://europepmc.org/articles/PMC2941302/

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Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells

Autor: Michelle Collins, Claudia Litterst, Adam M. McCoy, Luis A. Ugozzoli

Publikováno v: Journal of Visualized Experiments.

The use of siRNA mediated gene knockdown is continuing to be an important tool in studies of gene expression. siRNA studies are being conducted not only to study the effects of downregulating single genes, but also to interrogate signaling pathways a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::435c0bd7a93b156d4c9dee61b3fe48ff
https://doi.org/10.3791/1904-v

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Using the Gene Pulser MXcell Electroporation System to Transfect Primary Cells with High Efficiency

Autor: Luis A. Ugozzoli, Michelle Collins, Adam M. McCoy

Publikováno v: Journal of Visualized Experiments : JoVE

It is becoming increasingly apparent that electroporation is the most effective way to introduce plasmid DNA or siRNA into primary cells. The Gene Pulser MXcell electroporation system and Gene Pulser electroporation buffer (Bio-Rad) were specifically

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2f97beddcf06235b4fb231927c643160
https://doi.org/10.3791/1662-v

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