Zobrazeno 1 - 10
of 59
pro vyhledávání: '"Adam J. M. Wollman"'
Publikováno v:
Scientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
Abstract The yeast Hsp104 protein disaggregase is often used as a reporter for misfolded or damaged protein aggregates and protein quality control and ageing research. Observing Hsp104 fusions with fluorescent proteins is a popular approach to follow
Externí odkaz:
https://doaj.org/article/bafaf605c2364b1a9782e6ff88eb937a
Autor:
Manuel Saldivia, Adam J. M. Wollman, Juliana B. T. Carnielli, Nathaniel G. Jones, Mark C. Leake, Christopher Bower-Lepts, Srinivasa P. S. Rao, Jeremy C. Mottram
Publikováno v:
mBio, Vol 12, Iss 3 (2021)
In eukaryotic cells, kinetochores are large protein complexes that link chromosomes to dynamic microtubule tips, ensuring proper segregation and genomic stability during cell division. Several proteins tightly coordinate kinetochore functions, includ
Externí odkaz:
https://doaj.org/article/86905287e15d4286a26051507d71d700
Autor:
Helen Miller, Jason Cosgrove, Adam J. M. Wollman, Emily Taylor, Zhaokun Zhou, Peter J. O’Toole, Mark C. Coles, Mark C. Leake
Publikováno v:
Frontiers in Immunology, Vol 9 (2018)
Soluble factors are an essential means of communication between cells and their environment. However, many molecules readily interact with extracellular matrix components, giving rise to multiple modes of diffusion. The molecular quantification of di
Externí odkaz:
https://doaj.org/article/7566df7791d840c2b4d81cf3dcdded93
Publikováno v:
Open Biology, Vol 5, Iss 4 (2015)
Although not laying claim to being the inventor of the light microscope, Antonj van Leeuwenhoek (1632–1723) was arguably the first person to bring this new technological wonder of the age properly to the attention of natural scientists interested i
Externí odkaz:
https://doaj.org/article/785fadc9c80a4b0dbcec095ffa41deca
Autor:
Amelia Stennett, Kallie Friston, Claire L. Harris, Adam J. M. Wollman, Agnieszka K. Bronowska, Katrina S. Madden
Publikováno v:
Expert Opinion on Therapeutic Targets. 27:97-109
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::bf45eb6cefc44c141e776213d6238bcf
https://doi.org/10.1080/14728222.2023.2177532
https://doi.org/10.1080/14728222.2023.2177532
Publikováno v:
Computational and Structural Biotechnology Journal, Vol 19, Iss, Pp 4049-4058 (2021)
Computational and Structural Biotechnology Journal
Computational and Structural Biotechnology Journal
Graphical abstract
Highlights • We present PySTACHIO, a refined version of our spot tracking algorithm. • We demonstrate highly improved performance over previous MATLAB versions. • PySTACHIO can accurately estimate stoichiometries and 2D
Highlights • We present PySTACHIO, a refined version of our spot tracking algorithm. • We demonstrate highly improved performance over previous MATLAB versions. • PySTACHIO can accurately estimate stoichiometries and 2D
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::45fd988c44bc1bf2a6f9a821a51ae9ea
http://www.sciencedirect.com/science/article/pii/S2001037021002944
http://www.sciencedirect.com/science/article/pii/S2001037021002944
Autor:
Adam J M, Wollman, Mark C, Leake
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 2476
Single-molecule narrow-field microscopy is a versatile tool to investigate a diverse range of protein dynamics in live cells and has been extensively used in bacteria. Here, we describe how these methods can be extended to larger eukaryotic, yeast ce
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::9de9f4a5777d425a0ef267724e246d84
https://pubmed.ncbi.nlm.nih.gov/35635693
https://pubmed.ncbi.nlm.nih.gov/35635693
Autor:
Adam J. M. Wollman, Charlotte Fournier, Isabel Llorente-Garcia, Oliver Harriman, Alex L. Payne-Dwyer, Sviatlana Shashkova, Peng Zhou, Ta-Chun Liu, Djamila Ouaret, Jenny Wilding, Akihiro Kusumi, Walter Bodmer, Mark C. Leake
Publikováno v:
Journal of the Royal Society, Interface. 19(190)
Epidermal growth factor (EGF) signalling regulates normal epithelial and other cell growth, with EGF receptor (EGFR) overexpression reported in many cancers. However, the role of EGFR clusters in cancer and their dependence on EGF binding is unclear.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d6a54c2d41320b47edf27cd773eed198
https://pubmed.ncbi.nlm.nih.gov/35612280
https://pubmed.ncbi.nlm.nih.gov/35612280
Autor:
Mark C. Leake, Anthony J. Wilkinson, Zuzana Chromiková, Imrich Barák, Katarína Muchová, Adam J. M. Wollman
Publikováno v:
Computational and Structural Biotechnology Journal
Computational and Structural Biotechnology Journal, Vol 18, Iss, Pp 1474-1486 (2020)
Computational and Structural Biotechnology Journal, Vol 18, Iss, Pp 1474-1486 (2020)
Graphical abstract
Here we use singe-molecule optical proteomics and computational analysis of live cell bacterial images, using millisecond super-resolved tracking and quantification of fluorescently labelled protein SpoIIE in single live Bacil
Here we use singe-molecule optical proteomics and computational analysis of live cell bacterial images, using millisecond super-resolved tracking and quantification of fluorescently labelled protein SpoIIE in single live Bacil
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::276652fbcc11203f7c88db08ad3b53d7
http://europepmc.org/articles/PMC7327415
http://europepmc.org/articles/PMC7327415
Autor:
Adam J. M. Wollman, Mark C. Leake
Publikováno v:
Methods in Molecular Biology ISBN: 9781071622209
Methods in Molecular Biology ISBN: 9781493936298
Methods in Molecular Biology ISBN: 9781493936298
Single-molecule narrow-field microscopy is a versatile tool to investigate a diverse range of protein dynamics in live cells and has been extensively used in bacteria. Here, we describe how these methods can be extended to larger eukaryotic, yeast ce
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3e19932b6ee937b68311a4788e3fc747
https://doi.org/10.1007/978-1-0716-2221-6_2
https://doi.org/10.1007/978-1-0716-2221-6_2
