Zobrazeno 1 - 10
of 16
pro vyhledávání: '"Adam D Norris"'
Publikováno v:
eLife, Vol 8 (2019)
Single-cell transcriptomes are established by transcription factors (TFs), which determine a cell's gene-expression complement. Post-transcriptional regulation of single-cell transcriptomes, and the RNA binding proteins (RBPs) responsible, are more t
Externí odkaz:
https://doaj.org/article/693d9901788f4ee597a4c972cb15372c
Publikováno v:
eLife, Vol 6 (2017)
Genetic interaction screens have aided our understanding of complex genetic traits, diseases, and biological pathways. However, approaches for synthetic genetic analysis with null-alleles in metazoans have not been feasible. Here, we present a CRISPR
Externí odkaz:
https://doaj.org/article/df71ec85f2854922b915243b0cdc4528
Publikováno v:
Cell reports
SUMMARY Microexons (≤27 nt) play critical roles in nervous system development and function but create unique challenges for the splicing machinery. The mechanisms of microexon regulation are therefore of great interest. We performed a genetic scree
Autor:
Robert Dalton, Morgan Thompson, Alexa Vandenburg, John A. Calarco, Ryan Bixby, Adam D. Norris
Publikováno v:
eLife
eLife, Vol 8 (2019)
eLife, Vol 8 (2019)
Single-cell transcriptomes are established by transcription factors (TFs), which determine a cell's gene-expression complement. Post-transcriptional regulation of single-cell transcriptomes, and the RNA binding proteins (RBPs) responsible, are more t
Autor:
Adam D. Norris, John A. Calarco
Genetic interaction screens are a powerful methodology to establish novel roles for genes and elucidate functional connections between genes. Such studies have been performed to great effect in single-cell organisms such as yeast and E. coli (Schuldi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b51c5b619125f1a44043b75f33be668b
https://europepmc.org/articles/PMC5856468/
https://europepmc.org/articles/PMC5856468/
Efficient Genome Editing in Caenorhabditis elegans with a Toolkit of Dual-Marker Selection Cassettes
Publikováno v:
Genetics
Use of the CRISPR/Cas9 RNA-guided endonuclease complex has recently enabled the generation of double-strand breaks virtually anywhere in the C. elegans genome. Here, we present an improved strategy that makes all steps in the genome editing process m
Autor:
Arun K. Ramani, Megan L Norris, Mei Zhen, Debashish Ray, Shangbang Gao, John A. Calarco, Timothy P. Hughes, Adam D. Norris, Quaid Morris, Andrew G. Fraser
Publikováno v:
Molecular Cell. 54:946-959
Alternative splicing is important for the development and function of the nervous system, but little is known about the differences in alternative splicing between distinct types of neurons. Furthermore, the factors that control cell-type-specific sp
Publikováno v:
Advances in experimental medicine and biology. 907
Alternative pre-mRNA splicing serves as an elegant mechanism for generating transcriptomic and proteomic diversity between cell and tissue types. In this chapter, we highlight key concepts and outstanding goals in studies of tissue and cell-specific