Zobrazeno 1 - 7
of 7
pro vyhledávání: '"Adair Borges"'
Autor:
Yue Clare Lou, Ben Rubin, Marie Schoelmerich, Kaden DiMarco, Adair Borges, Rachel Rovinsky, Leo Song, Jennifer Doudna, Jillian Banfield
Human milk oligosaccharides (HMOs) ensure proper infant gut microbiome establishment. Isolate studies have revealed the genetic basis for HMO metabolism, but they exclude the possibility of HMO assimilation via synergistic interactions involving mult
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::0025da89ea8964cb3c9f51c99d6d3dbc
https://doi.org/10.21203/rs.3.rs-2743153/v1
https://doi.org/10.21203/rs.3.rs-2743153/v1
Autor:
Adair Borges
Cheese rind microbiomes are a validated and stable experimental platform for microbial community research. These communities are relatively simple, reproducible, and easy to grow in the lab. This set of protocols describes how to collect and store ch
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::4540480e83ec7f3f6fa0ac27c8154512
https://doi.org/10.17504/protocols.io.n2bvj8j5xgk5/v1
https://doi.org/10.17504/protocols.io.n2bvj8j5xgk5/v1
Autor:
Adair Borges
This protocol details how we harvest the viral component, or virome, from cheese rind microbiomes. These viromes can be banked at 4 °C for short-term storage or -80 °C for long-term storage. Alternatively, one can extract DNA or RNA from these viro
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::fa734dae419b662b5feec7dff67891e7
https://doi.org/10.17504/protocols.io.j8nlkw29xl5r/v1
https://doi.org/10.17504/protocols.io.j8nlkw29xl5r/v1
Autor:
Adair Borges
This protocol details high-molecular-weight DNA extraction from bacteriophages using phenol-chloroform. This protocol can be performed on a virome harvested from a microbial community, or a single phage amplified in the lab.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::5d28facc5ee8d21bad9f8e039074899a
https://doi.org/10.17504/protocols.io.ewov1oxdklr2/v1
https://doi.org/10.17504/protocols.io.ewov1oxdklr2/v1
Autor:
Adair Borges
This protocol details high-molecular-weight DNA extraction from bacteriophages using phenol-chloroform. Following DNA extraction, DNA is digested down to single nucleosides using the NEB Nucleoside Digestion Mix.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::6dca9b8861913848013aff7e548dc56b
https://doi.org/10.17504/protocols.io.8epv5jrxnl1b/v1
https://doi.org/10.17504/protocols.io.8epv5jrxnl1b/v1
Autor:
Adair Borges
This protocol details methods to amplify bacteriophages T4 and SPO1, and concentrate using either PEG precipitation or filtration.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::cd9a3f8a4b47d28be8ee1366dd3b71ee
https://doi.org/10.17504/protocols.io.yxmvmnb86g3p/v1
https://doi.org/10.17504/protocols.io.yxmvmnb86g3p/v1
Publikováno v:
J Bacteriol
Clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated (Cas) systems are prokaryotic adaptive immune systems that have been well characterized biochemically, but in vivo spatiotemporal regulation and cell biology remain