Zobrazeno 1 - 10
of 38
pro vyhledávání: '"Achim H. Krotz"'
Publikováno v:
Nucleosides, Nucleotides and Nucleic Acids. 24:1293-1299
A solution of sulfur (0.1 M) and sodium sulfide (0.01M) in 3-picoline, referred to as polysulfide reagent, rapidly converts trialkyl and triaryl phosphite triesters to the corresponding phosphorothioate derivatives. Greater than 99.8% average stepwis
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::61a8c082cac6ce917f1ac4f699062a7a
https://doi.org/10.1080/15257770500230384
https://doi.org/10.1080/15257770500230384
Autor:
Phil Olsen, Dennis Gorman, Achim H. Krotz, Hans Gaus, Anthony N. Scozzari, Claus Rentel, James V. McArdle
Publikováno v:
Nucleosides, Nucleotides and Nucleic Acids. 23:767-775
The impuritiy profiles of acetonitrile solutions of the four standard O-cyanoethyl-N,N-diisopropyl-phosphoramidites of 5'-O-dimethoxytrityl (DMT) protected deoxyribonucleosides (dG(ib), dA(bz), dC(bz), T) were analyzed by HPLC-MS. The solution stabil
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::05bfc73c731a8a91b0cd0482107ba9f0
https://doi.org/10.1081/ncn-120039215
https://doi.org/10.1081/ncn-120039215
Autor:
Achim H. Krotz, James D. Godbout, Dennis Gorman, Christopher C. Coffin, Anthony N. Scozzari, Craig Foster, Paul Mataruse
Publikováno v:
Organic Process Research & Development. 8:852-858
In this report, we describe the design of an efficient and robust sulfurization step in the production process of phosphorothioate (PS) oligonucleotides by solid-phase synthesis. Average stepwise s...
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::6b1cc89cf2366b29c01ab469f4f0d497
https://doi.org/10.1021/op040208v
https://doi.org/10.1021/op040208v
Autor:
Hans Gaus, Stella D. Decottignies, Max N. Moore, Vasulinga T. Ravikumar, Brett Turney, Achim H. Krotz, James V. McArdle, Anthony N. Scozzari, Daniel C. Capaldi, Recaldo L. Carty
Publikováno v:
Bioorganic & Medicinal Chemistry Letters. 14:4683-4690
Incomplete sulfurization during solid-phase synthesis of phosphorothioate oligonucleotides using phosphoramidite chemistry was identified as the cause of formation of two new classes of process-related oligonucleotide impurities containing a DMTr-C-p
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::48931352b7dd213f04cb38f064c47fc6
https://doi.org/10.1016/j.bmcl.2004.06.088
https://doi.org/10.1016/j.bmcl.2004.06.088
Publikováno v:
Organic Process Research & Development. 8:271-274
During solid-phase synthesis of oligonucleotides in packed-bed reactors a substantial pressure build-up is observed during acid deprotection and subsequent solvent washing as the length of the supp...
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::f96a5df536dc8f3e1c40b915d9743e3a
https://doi.org/10.1021/op030213j
https://doi.org/10.1021/op030213j
Autor:
Kirsten Lowery, Daniel C. Capaldi, Achim H. Krotz, Max N. Moore, Hans Gaus, Vasulinga T. Ravikumar, Anthony N. Scozzari, Ricaldo L. Carty, Jim Arnold, Douglas L. Cole
Publikováno v:
Organic Process Research & Development. 7:832-838
It is demonstrated that the acrylonitrile (AN) generated during the ammonolysis step of oligonucleotide manufacture selectively adds to thymine residue present in ISIS 2302 to give a full-length ol...
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::2231f3c9be23bf5a46e3f1f6736e5ba2
https://doi.org/10.1021/op020090n
https://doi.org/10.1021/op020090n
Autor:
Bethany M. McElroy, Achim H. Krotz, Douglas L. Cole, Vasulinga T. Ravikumar, Anthony N. Scozzari
Publikováno v:
Organic Process Research & Development. 7:47-52
The last chemical step in standard oligonucleotide synthesis, the acid-catalyzed removal of 4,4‘-dimethoxytrityl (DMTr) protecting groups from the 5‘-terminus of oligonucleotides is accompanied by hydrolysis of purine nucleoside glycosidic linkag
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::7df97c7b12b56b7bdb70e5b7d13357aa
https://doi.org/10.1021/op020068u
https://doi.org/10.1021/op020068u
Publikováno v:
Bioorganic & Medicinal Chemistry Letters. 11:1863-1867
High-quality oligonucleotides are obtained by selective modification of sequences containing aldehyde apurinic sites with a new chromatographic tag followed by RP-HPLC separation. Hydroxylamine derivative 1 of a water soluble nonionic surfactant modi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f5c00468ca4146628bcd8ca6202bab27
https://doi.org/10.1016/s0960-894x(01)00307-9
https://doi.org/10.1016/s0960-894x(01)00307-9
Autor:
Daniel C. Capaldi, Achim H. Krotz, Vasulinga T. Ravikumar, Alessandra Eleuteri, and Douglas L. Cole
Publikováno v:
Organic Process Research & Development. 4:182-189
A new activator is reported for coupling phosphoramidites to a free 5‘-hydroxyl group during oligonucleotide synthesis. Pyridinium trifluoroacetate/N-methyl imidazole is a remarkably efficient replacement for 1H-tetrazole in the solid-supported syn
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ca5fff4fa97b19b8b176a647cd1f803e
https://doi.org/10.1021/op9900378
https://doi.org/10.1021/op9900378
Autor:
Recaldo L. Carty, and Douglas L. Cole, Achim H. Krotz, Vasulinga T. Ravikumar, Anthony N. Scozzari
Publikováno v:
Organic Process Research & Development. 4:190-193
It is demonstrated that mixed-sequence phosphorothioate oligodeoxyribonucleotides can be synthesized on scales from 1 μmol up to 80 mmol without using chlorinated solvents such as dichloromethane o...
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::0c366d422a38625fe85249c1116144b8
https://doi.org/10.1021/op990183d
https://doi.org/10.1021/op990183d