Tailor-Made α-Glucans by Engineering the Processivity of α-Glucanotransferases via Tunnel-Cleft Active Center Interconversions.

Autor: Dong J; State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, Jiangsu 214122, China.; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China.; Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, Jiangnan University, Wuxi, Jiangsu 214122, China., Abou Hachem M; Department of Biotechnology and Biomedicine, Technical University of Denmark, DK-2800 Kongens Lyngby, Denmark., Wang Y; College of Food Sciences and Engineering, Ningbo University, Ningbo, Zhejiang 315832, China., Li X; State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, Jiangsu 214122, China.; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China.; Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, Jiangnan University, Wuxi, Jiangsu 214122, China., Zhang B; State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, Jiangsu 214122, China.; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China.; Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, Jiangnan University, Wuxi, Jiangsu 214122, China., Pijning T; Biomolecular X-ray Crystallography, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, 9747 AG Groningen, The Netherlands., Svensson B; Department of Biotechnology and Biomedicine, Technical University of Denmark, DK-2800 Kongens Lyngby, Denmark., Dijkhuizen L; Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, 9747 AG Groningen, The Netherlands.; CarbExplore Research BV, 9747 AA Groningen, The Netherlands., Jin Z; State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, Jiangsu 214122, China.; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China.; Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, Jiangnan University, Wuxi, Jiangsu 214122, China., Bai Y; State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, Jiangsu 214122, China.; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China.; Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, Jiangnan University, Wuxi, Jiangsu 214122, China.; International Joint Laboratory on Food Safety, Jiangnan University, Wuxi, Jiangsu 214122, China.

Publikováno v: Journal of agricultural and food chemistry [J Agric Food Chem] 2024 May 15; Vol. 72 (19), pp. 11041-11050. Date of Electronic Publication: 2024 May 03.

Insights into the Structure-Function Relationship of GH70 GtfB α-Glucanotransferases from the Crystal Structure and Molecular Dynamic Simulation of a Newly Characterized Limosilactobacillus reuteri N1 GtfB Enzyme.

Autor: Dong J; State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, Jiangsu 214122, China.; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China.; Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, Jiangnan University, Wuxi, Jiangsu 214122, China., Bai Y; State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, Jiangsu 214122, China.; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China.; Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, Jiangnan University, Wuxi, Jiangsu 214122, China.; International Joint Laboratory on Food Safety, Jiangnan University, Wuxi, Jiangsu 214122, China., Wang Q; Department of Biochemistry and Molecular Biology, Binzhou Medical University, YanTai, Shandong 264003, China., Chen Q; State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, Jiangsu 214122, China.; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China.; Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, Jiangnan University, Wuxi, Jiangsu 214122, China., Li X; State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, Jiangsu 214122, China.; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China.; Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, Jiangnan University, Wuxi, Jiangsu 214122, China., Wang Y; College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo, Zhejiang 315832, China., Ji H; State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, Jiangsu 214122, China.; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China.; Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, Jiangnan University, Wuxi, Jiangsu 214122, China., Meng X; State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, Jinan 250100, China., Pijning T; Biomolecular X-ray Crystallography, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, 9747 AG Groningen, The Netherlands., Svensson B; Department of Biotechnology and Biomedicine, Technical University of Denmark, DK-2800 Kgs. Lyngby, Denmark., Dijkhuizen L; Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, 9747 AG Groningen, The Netherlands.; CarbExplore Research BV, 9747 AA Groningen, The Netherlands., Abou Hachem M; Department of Biotechnology and Biomedicine, Technical University of Denmark, DK-2800 Kgs. Lyngby, Denmark., Jin Z; State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, Jiangsu 214122, China.; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China.; Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, Jiangnan University, Wuxi, Jiangsu 214122, China.

Publikováno v: Journal of agricultural and food chemistry [J Agric Food Chem] 2024 Mar 13; Vol. 72 (10), pp. 5391-5402. Date of Electronic Publication: 2024 Mar 01.

Sialidases and fucosidases of Akkermansia muciniphila are crucial for growth on mucin and nutrient sharing with mucus-associated gut bacteria.

Autor: Shuoker B; Department of Biotechnology and Biomedicine, Technical University of Denmark, Lyngby, 2800, Denmark.; Biotechnology, Department of Chemistry, Lund University, Lund, Sweden., Pichler MJ; Department of Biotechnology and Biomedicine, Technical University of Denmark, Lyngby, 2800, Denmark., Jin C; Proteomics Core Facility at Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden., Sakanaka H; Department of Biotechnology and Biomedicine, Technical University of Denmark, Lyngby, 2800, Denmark., Wu H; Quadram Institute Bioscience, Norwich, UK., Gascueña AM; Quadram Institute Bioscience, Norwich, UK., Liu J; Department of Laboratory Medicine, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden., Nielsen TS; Department of Biotechnology and Biomedicine, Technical University of Denmark, Lyngby, 2800, Denmark., Holgersson J; Department of Laboratory Medicine, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden., Nordberg Karlsson E; Biotechnology, Department of Chemistry, Lund University, Lund, Sweden., Juge N; Quadram Institute Bioscience, Norwich, UK., Meier S; Department of Chemistry, Technical University of Denmark, Kgs Lyngby, Denmark., Morth JP; Department of Biotechnology and Biomedicine, Technical University of Denmark, Lyngby, 2800, Denmark. premo@dtu.dk., Karlsson NG; Proteomics Core Facility at Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden., Abou Hachem M; Department of Biotechnology and Biomedicine, Technical University of Denmark, Lyngby, 2800, Denmark. maha@bio.dtu.dk.

Publikováno v: Nature communications [Nat Commun] 2023 Apr 01; Vol. 14 (1), pp. 1833. Date of Electronic Publication: 2023 Apr 01.

Generation of Multivalent Nanobody-Based Proteins with Improved Neutralization of Long α-Neurotoxins from Elapid Snakes.

Autor: Wade J; Department of Biotechnology and Biomedicine, Technical University of Denmark, DK-2800 Kongens, Lyngby, Denmark., Rimbault C; Department of Biotechnology and Biomedicine, Technical University of Denmark, DK-2800 Kongens, Lyngby, Denmark., Ali H; Quadrucept Bio Ltd., Kemp House, 152 City Road, London EC1V 2NX, United Kingdom., Ledsgaard L; Department of Biotechnology and Biomedicine, Technical University of Denmark, DK-2800 Kongens, Lyngby, Denmark., Rivera-de-Torre E; Department of Biotechnology and Biomedicine, Technical University of Denmark, DK-2800 Kongens, Lyngby, Denmark., Abou Hachem M; Department of Biotechnology and Biomedicine, Technical University of Denmark, DK-2800 Kongens, Lyngby, Denmark., Boddum K; Sophion Bioscience, DK-2750 Ballerup, Denmark., Mirza N; Fida Biosystems ApS, DK-2860 Søborg, Copenhagen, Denmark., Bohn MF; Department of Biotechnology and Biomedicine, Technical University of Denmark, DK-2800 Kongens, Lyngby, Denmark., Sakya SA; Department of Immunology, Oslo University Hospital Rikshospitalet, N-0372 Oslo, Norway.; Department of Pharmacology, Institute of Clinical Medicine, University of Oslo, N-0372 Oslo, Norway., Ruso-Julve F; Department of Immunology, Oslo University Hospital Rikshospitalet, N-0372 Oslo, Norway.; Department of Pharmacology, Institute of Clinical Medicine, University of Oslo, N-0372 Oslo, Norway., Andersen JT; Department of Immunology, Oslo University Hospital Rikshospitalet, N-0372 Oslo, Norway.; Department of Pharmacology, Institute of Clinical Medicine, University of Oslo, N-0372 Oslo, Norway., Laustsen AH; Department of Biotechnology and Biomedicine, Technical University of Denmark, DK-2800 Kongens, Lyngby, Denmark.

Publikováno v: Bioconjugate chemistry [Bioconjug Chem] 2022 Aug 17; Vol. 33 (8), pp. 1494-1504. Date of Electronic Publication: 2022 Jul 23.

Two binding proteins of the ABC transporter that confers growth of Bifidobacterium animalis subsp. lactis ATCC27673 on β‐mannan possess distinct manno‐oligosaccharide‐binding profiles.

Autor: Ejby, M., Guskov, A., Pichler, M. J., Zanten, G. C., Schoof, E., Saburi, W., Slotboom, D. J., Abou Hachem, M.

Publikováno v: Molecular Microbiology; Jul2019, Vol. 112 Issue 1, p114-130, 17p, 2 Color Photographs, 5 Charts, 4 Graphs